Long-term cytotoxicities of various pesticides evaluated by albumin secretion of primary cultured rat hepatocytes

1996 ◽  
Vol 10 (2) ◽  
pp. 99-102 ◽  
Author(s):  
Kazuhiro Ichikawa ◽  
Yasuyuki Sakai ◽  
Akiyoshi Sakoda ◽  
Motoyuki Suzuki
Keyword(s):  
Rat Hepatocytes ◽  
Albumin Secretion ◽  
Primary Cultured Rat Hepatocytes ◽  
Cultured Rat Hepatocytes

The Use of Primary Cultured Rat Hepatocytes for the Assessment of Xenobiotic Effects on Biotransformation

1991 ◽  
Vol 19 (2) ◽  
pp. 209-213
Author(s):  
Gabi Schepers ◽  
Christiane Aschmann ◽  
Sabine Mörchel
Keyword(s):  
Cell Viability ◽  
Rat Hepatocytes ◽  
Standard Test ◽  
In Vitro Test ◽  
Chlorinated Phenols ◽  
Test Protocol ◽  
Primary Cultured Rat Hepatocytes ◽  
Cultured Rat Hepatocytes ◽  
Different Response

An in vitro test protocol is reported, which, using primary cultured rat hepatocytes, allows for the screening of xenobiotic effects on biotransformation as well as on basal cellular functions. O-Deethylation of 7-ethoxycoumarin (7-EC) and subsequent conjugation of the metabolite 7-hydroxycoumarin (7-HC) with sulphate or glucuronic acid are determined, as representative parameters for the hepatic biotransformation. Cell viability is examined by measuring cellular ATP content and leakage of lactate dehydrogenase. With respect to immediate and delayed effects on biotransformation reactions, the standard test protocol includes exposure to xenobiotics for 1, 24 and 48 hours. Different response patterns could be demonstrated for the solvents dimethylformamide (DMF) and dimethylsulphoxide (DMSO), and the chlorinated phenols, pentachlorophenol (PCP) and hexachlorophene (HCP), which are known to uncouple mitochondrial respiration. Short-term incubation with the solvents resulted in decreased 7-EC- O-deethylation without signs of cytotoxicity. PCP and HCP inhibited 7-EC- O-deethylation and 7-HC-conjugation, affecting sulphate and glucuronide formation differently. 24-hour exposures to PCP and HCP resulted in decreased 7-ethoxycoumarin- O-deethylase activity, which correlated with diminished cell viability, while DMSO and DMF enhanced 7-EC- O-deethylation at sub-cytotoxic concentrations. After exposure for 48 hours to the solvents, enzyme induction was even more pronounced.

Metabolic interactions between ethanol and MDMA in primary cultured rat hepatocytes

Toxicology ◽  
2010 ◽  
Vol 270 (2-3) ◽  
pp. 150-157 ◽  
Author(s):  
Helena Pontes ◽  
Paula Guedes de Pinho ◽  
Eduarda Fernandes ◽  
Paula Sério Branco ◽  
Luísa Maria Ferreira ◽  
...  
Keyword(s):  
Rat Hepatocytes ◽  
Primary Cultured Rat Hepatocytes ◽  
Metabolic Interactions ◽  
Cultured Rat Hepatocytes

scholarly journals Mechanism of Calcium Waves in Primary Cultured Rat Hepatocytes - Relationship with Distribution of Receptor

1996 ◽  
Vol 71 ◽  
pp. 164
Author(s):  
Toru KAWANISHI ◽  
Satoru ISHIZAKI ◽  
Takehito KIUCHI ◽  
Miyako OHTA ◽  
Hisayuki OHATA ◽  
...  
Keyword(s):  
Rat Hepatocytes ◽  
Calcium Waves ◽  
Primary Cultured Rat Hepatocytes ◽  
Cultured Rat Hepatocytes
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