Evaluation of monitoring approaches and effects of culture conditions on recombinant protein production in baculovirus-infected insect cells

1994 ◽  
Vol 15 (1-3) ◽  
pp. 177-186 ◽  
Author(s):  
William T. Hensler ◽  
Spiros N. Agathos
Keyword(s):  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Insect Cells ◽  
Culture Conditions ◽  
Infected Insect

scholarly journals The Goldilocks Approach: A Review of Employing Design of Experiments in Prokaryotic Recombinant Protein Production

2018 ◽  
Vol 5 (4) ◽  
pp. 89 ◽  
Author(s):  
Albert Uhoraningoga ◽  
Gemma Kinsella ◽  
Gary Henehan ◽  
Barry Ryan
Keyword(s):  
Recombinant Protein ◽  
Design Of Experiments ◽  
Prokaryotic Expression ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Expression System ◽  
Culture Conditions ◽  
Media Composition ◽  
High Yields ◽  
Soluble Recombinant Protein

The production of high yields of soluble recombinant protein is one of the main objectives of protein biotechnology. Several factors, such as expression system, vector, host, media composition and induction conditions can influence recombinant protein yield. Identifying the most important factors for optimum protein expression may involve significant investment of time and considerable cost. To address this problem, statistical models such as Design of Experiments (DoE) have been used to optimise recombinant protein production. This review examines the application of DoE in the production of recombinant proteins in prokaryotic expression systems with specific emphasis on media composition and culture conditions. The review examines the most commonly used DoE screening and optimisation designs. It provides examples of DoE applied to optimisation of media and culture conditions.

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