Characterization of the inhibitor sensitivity of the coenzyme a transport system in isolated rat heart mitochondria

1992 ◽  
Vol 24 (6) ◽  
pp. 635-640 ◽  
Author(s):  
Arun G. Tahiliani ◽  
Timothy Keene ◽  
Ronald S. Kaplan
Keyword(s):  
Transport System ◽  
Rat Heart ◽  
Coenzyme A ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
Rat Heart Mitochondria ◽  
Isolated Rat

Effects of pinacidil and calcium on isolated rat heart mitochondria

2012 ◽  
Vol 443 (1) ◽  
pp. 113-117 ◽  
Author(s):  
S. M. Korotkov ◽  
L. V. Emel’yanova ◽  
I. V. Brailovskaya ◽  
V. P. Nesterov
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
Rat Heart Mitochondria ◽  
Isolated Rat

Uncoupling and Antioxidant Effects of Ursolic Acid in Isolated Rat Heart Mitochondria

2011 ◽  
Vol 74 (7) ◽  
pp. 1640-1644 ◽  
Author(s):  
Julius Liobikas ◽  
Daiva Majiene ◽  
Sonata Trumbeckaite ◽  
Lolita Kursvietiene ◽  
Ruta Masteikova ◽  
...  
Keyword(s):  
Ursolic Acid ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
Rat Heart Mitochondria ◽  
Antioxidant Effects ◽  
Isolated Rat

scholarly journals Celecoxib Decreases Mitochondrial Complex IV Activity and Induces Oxidative Stress in Isolated Rat Heart Mitochondria: An Analysis for its Cardiotoxic Adverse Effect

2020 ◽  
Author(s):  
Saman Atashbar ◽  
Elham Mohammad Khanlou ◽  
Saleh Khezri ◽  
Peyman Kurdpour ◽  
Ahmad Salimi
Keyword(s):  
Lipid Peroxidation ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Mitochondrial Swelling ◽  
Heart Mitochondria ◽  
Isolated Mitochondria ◽  
Biochemical Assays ◽  
Ros Formation ◽  
Rat Heart Mitochondria ◽  
Isolated Rat

Abstract Background In spite of the cardiotoxic effect of selective cyclooxygenase-2 inhibitors, they are most widely used as anti-inflammatory and analgesic drugs. Today, valdecoxib and rofecoxib have been withdrawn on the market but celecoxib remains. In this study, we focused on an analysis of celecoxib toxic effects on isolated mitochondrial. Methods isolated rat heart mitochondria were obtained using differential centrifugation. Using flowcytometry and biochemical assays we searched succinate dehydrogenases (SDH), mitochondrial membrane potential (MMP), reactive oxygen species (ROS) formation, mitochondrial swelling, lipid peroxidation and mitochondrial complexes activity in rat heart isolated mitochondria. Results In here our results indicated a significant decrease in activity of complexes IV after exposure with celecoxib (16 µg/ml). This decrease in activity of complexes IV is paralleled by the MMP collapse, ROS formation, mitochondrial swelling and lipid peroxidation. Conclusion For the first time, this introductory study has showed a significant decrease in activity of complexes IV and mitochondrial dysfunction after exposure with celecoxib in rat heart isolated mitochondria.

Sign in / Sign up

Export Citation Format

Share Document