Phosgene effects on F-actin organization and concentration in cells cultured from sheep and rat lung

1994 ◽  
Vol 10 (1) ◽  
pp. 45-58 ◽  
Author(s):  
R. J. Werrlein ◽  
J. S. Madren-Whalley ◽  
S. D. Kirby
Keyword(s):  
Rat Lung ◽  
Actin Organization ◽  
Cells Cultured ◽  
In Cells

scholarly journals 3P189 Movement of nucleus in cells cultured on micro-patterned substrates(Cell biology,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

2014 ◽  
Vol 54 (supplement1-2) ◽  
pp. S280
Author(s):  
Zhu Xinfeng ◽  
Kuribayashi-Shigetomi Kaori ◽  
Cai Pinggen ◽  
Subagyo Agus ◽  
Sueoka Kazuhisa ◽  
...  
Keyword(s):  
Annual Meeting ◽  
Cell Biology ◽  
Patterned Substrates ◽  
Biophysical Society ◽  
Cells Cultured ◽  
In Cells

Intrinsic mechanical properties of the extracellular matrix affect the behavior of pre-osteoblastic MC3T3-E1 cells

2006 ◽  
Vol 290 (6) ◽  
pp. C1640-C1650 ◽  
Author(s):  
Chirag B. Khatiwala ◽  
Shelly R. Peyton ◽  
Andrew J. Putnam
Keyword(s):  
Mechanical Properties ◽  
Type I Collagen ◽  
Focal Adhesions ◽  
Microscopic Analysis ◽  
Maximal Activity ◽  
Type I ◽  
Cell Functions ◽  
Collagen Density ◽  
Cells Cultured ◽  
In Cells

Mechanical cues present in the ECM have been hypothesized to provide instructive signals that dictate cell behavior. We probed this hypothesis in osteoblastic cells by culturing MC3T3-E1 cells on the surface of type I collagen-modified hydrogels with tunable mechanical properties and assessed their proliferation, migration, and differentiation. On gels functionalized with a low type I collagen density, MC3T3-E1 cells cultured on polystyrene proliferated twice as fast as those cultured on the softest substrate. Quantitative time-lapse video microscopic analysis revealed random motility speeds were significantly retarded on the softest substrate (0.25 ± 0.01 μm/min), in contrast to maximum speeds on polystyrene substrates (0.42 ± 0.04 μm/min). On gels functionalized with a high type I collagen density, migration speed exhibited a biphasic dependence on ECM compliance, with maximum speeds (0.34 ± 0.02 μm/min) observed on gels of intermediate stiffness, whereas minimum speeds (0.24 ± 0.03 μm/min) occurred on both the softest and most rigid (i.e., polystyrene) substrates. Immature focal contacts and a poorly organized actin cytoskeleton were observed in cells cultured on the softest substrates, whereas those on more rigid substrates assembled mature focal adhesions and robust actin stress fibers. In parallel, focal adhesion kinase (FAK) activity (assessed by detecting pY397-FAK) was influenced by compliance, with maximal activity occurring in cells cultured on polystyrene. Finally, mineral deposition by the MC3T3-E1 cells was also affected by ECM compliance, leading to the conclusion that altering ECM mechanical properties may influence a variety of MC3T3-E1 cell functions, and perhaps ultimately, their differentiated phenotype.

scholarly journals Mutations in ras genes in cells cultured from mouse skin tumors induced by ultraviolet irradiation.

1994 ◽  
Vol 91 (15) ◽  
pp. 7189-7193 ◽  
Author(s):  
C. Nishigori ◽  
S. Wang ◽  
J. Miyakoshi ◽  
M. Sato ◽  
T. Tsukada ◽  
...  
Keyword(s):  
Ultraviolet Irradiation ◽  
Mouse Skin ◽  
Skin Tumors ◽  
Ras Genes ◽  
Cells Cultured ◽  
In Cells

Evaluation of the Effect of 1,3-Bis(4-Phenyl)-1H-1,2,3-Triazolyl-2-Propanolol on Gene Expression Levels of JAK2–STAT3, NF-κB, and SOCS3 in Cells Cultured from Biopsies of Mammary Lesions

2015 ◽  
Vol 53 (11-12) ◽  
pp. 291-300 ◽  
Author(s):  
J. L. Malvaez Becerril ◽  
J. G. Santillán Benítez ◽  
J. J. Torres Juárez ◽  
J. M. González Bañales ◽  
H. Mendieta Zerón ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Levels ◽  
Gene Expression Levels ◽  
Cells Cultured ◽  
In Cells

Role of miR-214 in biomaterial transplantation therapy for osteonecrosis

2021 ◽  
pp. 1-13
Author(s):  
Yuying Wang ◽  
Rui He ◽  
Anqi Yang ◽  
Rui Guo ◽  
Jie Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Bone Scaffold ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells ◽  
Transplantation Therapy ◽  
Cells Cultured ◽  
In Cells

BACKGROUND: The effectiveness and availability of conservative therapies for osteonecrosis of the femoral head (ONFH) are limited. Transplantation of bone marrow mesenchymal stem cells (BMSCs) combined with Bio-Oss, which is a good bone scaffold biomaterial for cell proliferation and differentiation, is a new potential therapy. Of note, the expression of miRNAs was significantly modified in cells cultured with Bio-Oss, and MiR-214 was correlated positively with osteonecrosis. Furthermore, miR-214 was upregulated in cells exposed to Bio-Oss. OBJECTIVE: To investigate whether targeting miR-214 further improves the transplantation effect. METHODS: We treated BMSCs with agomiR-214 (a miR-214 agonist), antagomiR-214 (a miR-214 inhibitor), or vehicle, followed by their transplantation into ONFH model rats. RESULTS: Histological and histomorphometric data showed that bone formation was significantly increased in the experimental groups (Bio-Oss and BMSCs treated with antagomiR-214) compared with other groups. CONCLUSIONS: miR-214 participates in the inhibition of osteoblastic bone formation, and the inhibition of miR-214 to bone formation during transplantation therapy with Bio-Oss combined with BMSCs for ONFH.

Rho, Rac and Cdc42 regulate actin organization and cell adhesion in macrophages

1997 ◽  
Vol 110 (6) ◽  
pp. 707-720 ◽  
Author(s):  
W.E. Allen ◽  
G.E. Jones ◽  
J.W. Pollard ◽  
A.J. Ridley
Keyword(s):  
Actin Reorganization ◽  
Membrane Ruffling ◽  
Actin Organization ◽  
Phosphorylated Proteins ◽  
Adhesion Sites ◽  
Actin Cable ◽  
Cable Assembly ◽  
Beta1 Integrin ◽  
Actin Cables ◽  
In Cells

Rho family proteins are known to regulate actin organization in fibroblasts, but their functions in cells of haematopoietic origin have not been studied in detail. Bac1.2F5 cells are a colony-stimulating factor-1 (CSF-1)-dependent murine macrophage cell line; CSF-1 stimulates their proliferation and motility, and acts as a chemoattractant. CSF-1 rapidly induced actin reorganization in Bac1 cells: it stimulated the formation of filopodia, lamellipodia and membrane ruffles at the plasma membrane, as well as the appearance of fine actin cables within the cell interior. Microinjection of constitutively activated (V12)Rac1 stimulated lamellipodium formation and membrane ruffling. The dominant inhibitory Rac mutant, N17Rac1, inhibited CSF-1-induced lamellipodium formation, and also induced cell rounding. V12Cdc42 induced the formation of long filopodia, while the dominant inhibitory mutant N17Cdc42 prevented CSF-1-induced formation of filopodia but not lamellipodia. V14RhoA stimulated actin cable assembly and cell contraction, while the Rho inhibitor, C3 transferase, induced the loss of actin cables. Bac1 cells had cell-to-substratum adhesion sites containing beta1 integrin, pp125FAK, paxillin, vinculin, and tyrosine phosphorylated proteins. These ‘focal complexes’ were present in growing and CSF-1-starved cells, but were disassembled in cells injected with N17Cdc42 or N17Rac1. Interestingly, beta1 integrin did not disperse until long after focal phosphotyrosine and vinculin staining had disappeared. We conclude that in Bac1 macrophages Cdc42, Rac and Rho regulate the formation of distinct actin filament-based structures, and that Cdc42 and Rac are also required for the assembly of adhesion sites to the extracellular matrix.

Kinetic characterization of ATP diphosphohydrolase and 5′-nucleotidase activities in cells cultured from submandibular salivary glands of rats

2006 ◽  
Vol 30 (3) ◽  
pp. 214-220 ◽  
Author(s):  
Sandra Liana Henz ◽  
Cristiane Guimarães Ribeiro ◽  
Aline Rosa ◽  
Rafael Augusto Chiarelli ◽  
Emerson André Casali ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Kinetic Characterization ◽  
Atp Diphosphohydrolase ◽  
Submandibular Salivary Glands ◽  
Cells Cultured ◽  
In Cells
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