Effects of dibutyryl cAMP and bromodeoxyuridine on expression ofN-acetylglucosaminyltransferases III and V in GOTO neuroblastoma cells

1995 ◽  
Vol 12 (6) ◽  
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Atsushi Nishikawa ◽  
Naoyuki Taniguchi
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1982 ◽  
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T KATO ◽  
Y SAKAZAKI ◽  
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...  

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Vol 29 (03) ◽  
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B. Kornhuber ◽  
E. Busse

The effect of triiodothyronine (T3) on the differentiation of cultured neuroblastoma (NB) cells was studied after 9 days of treatment with a dose of 10-4 M/106 cells per day. Using phase contrast microscopy, 30-50% of NB cells showed formation of neurites as a morphological sign of cellular differentiation. The initial rise of the mitosis rate was followed by a plateau. Changes in cyclic nucleotide content, in the triphosphates and in the activity of the enzyme ornithine decarboxylase (ODC) were assessed in 2 human and 2 murine cell lines to serve as biochemical parameters of the cell differentiation induced by T3. Whereas the cAMP level increased significantly (3 to 7 fold compared with its initial value), the cGMP value dropped to 30 to 50% of that of the control group. ATP and GTP increased about 200%, the ODC showed a decrease of about 50%. The present studies show a biphasic effect of T3 on neuroblastoma cells: the initial rise of mitotic activity is followed by increased cell differentiation starting from day 4 of the treatment.


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