Grinding of fine granular material with fluidization

1972 ◽  
Vol 8 (3) ◽  
pp. 210-213 ◽  
Author(s):  
T. A. Kutyavina ◽  
A. P. Baskakov
1989 ◽  
Vol 37 (6) ◽  
pp. 801-811 ◽  
Author(s):  
A H Salama ◽  
A E Zaki ◽  
D R Eisenmann

Trimetaphosphatase (TMPase) and cytidine-5'-monophosphatase (CMPase) were localized to investigate the lysosomal system, particularly tubular lysosomes, in ruffle-ended ameloblasts associated with maturation of enamel in rat incisor. Demineralized specimens were incubated for TMPase and for CMPase in a modified medium where cerium was used as the capture ion. Ruffle-ended ameloblasts showed distal invaginations and membrane-bound bodies filled with fine granular material, some of which displayed CMPase reaction product. Elongated tubular configurations 80-140 nm wide were distributed throughout the cytoplasm and were reactive with both TMPase and CMPase, thus characterizing these structures as lysosomes. They often contained fine granular material morphologically similar to that present in multivesicular bodies. During late enamel maturation, fewer tubular lysosomes were observed when compared to early maturation. These cytochemical results demonstrate the presence of tubular lysosomes in ruffle-ended ameloblasts, and it is suggested that they are elements of the endosomal system in these cells. These findings are also consistent with a resorptive function for ruffle-ended ameloblasts during enamel maturation.


2003 ◽  
Vol 23 (4) ◽  
pp. 183-211 ◽  
Author(s):  
P. Wodzinski

2003 ◽  
Vol 23 (4) ◽  
pp. 185-213 ◽  
Author(s):  
P. Wodzinski

2006 ◽  
Vol 61 (8) ◽  
pp. 2394-2405 ◽  
Author(s):  
A.V. Matveev ◽  
L.V. Barysheva ◽  
I.V. Koptyug ◽  
V.M. Khanaev ◽  
A.S. Noskov

1969 ◽  
Vol 43 (3) ◽  
pp. 553-563 ◽  
Author(s):  
Awtar Krishan ◽  
Dora Hsu

Earle's L-929 fibroblasts from cultures treated with 5–10 µg/ml of vincristine sulfate have a large number of eosinophilic, proteinaceous crystals in their cytoplasm. In electron micrographs, large arrays of helical polyribosomes, stacks of Golgi lamellae, and membranes of granular endoplasmic reticulum are seen in the cytoplasm of these cells. "Stalks" of fine granular material, approximately 300 A wide, are often seen in association with the arrays of the helical polyribosomes. In many sections rows of helical polyribosomes and filaments emerging from individual polyribosomes are seen in intimate contact with the crystals. A gradual reduction in the number of crystals and crystal-bearing cells is seen in cultures removed from the drug-containing medium and reincubated in fresh medium. In electron micrographs, these reincubated cells show large aggregates of filamentous material in the cytoplasm, and in many sections filaments are seen in continuity with the crystals.


1987 ◽  
Vol 24 (5) ◽  
pp. 436-443 ◽  
Author(s):  
A. M. Bendele ◽  
S. L. White

The articular cartilage from femorotibial joints of partial medial meniscectomized male guinea pigs was evaluated at 24, 48, 72, and 96 hours post-surgery to determine the sequential histopathologic and ultrastructural alterations. At 24 hours post-surgery, histopathologic alterations were in the superficial and middle layers and consisted of degeneration and necrosis of chondrocytes and minimal decreased intensity of toluidine blue matrix staining. Changes in chondrocytes and matrix became progressively more extensive 48 hours after surgery. Ultrastructurally, the changes in the superficial matrix appeared to be the result of loss of the fine granular material interspersed between collagen fibers. At 72 and 96 hours post-surgery, chondrocyte loss was extensive and surface fibrillation was seen. These findings suggested that chondrocyte death was the initial important event which led to progressive severe cartilage degeneration in this model.


Parasitology ◽  
1969 ◽  
Vol 59 (1) ◽  
pp. 193-201 ◽  
Author(s):  
David A. Erasmus

The ultrastructure of the lappets of Apatemon gracilis minor Yamaguti, 1933, has been described. The tegument covering the lappets is characterized by the presence of long seta-like structures and unicellular gland cells which exhibit a holocrine type of secretion. The setae contain extensions of the tegument cytoplasm and are supported by a bundle of fibrils. The unicellular gland cells are pear-shaped with the tapering neck supported by a ring of 50–60 microtubules. The secretion consists of relatively large, membrane bounded bodies containing a fine granular material.The lappets are regarded as representing a specialized host–parasite interface in which adaptation exists for attachment to the host tissue and the discharge of secretion from the parasite at this region. The concept of the specialized host–parasite interface is discussed and extended to include Apatemon gracilis minor.The author wishes to acknowledge the research grant provided by the S.R.C. for the purchase of a vacuum coating unit and an AEI EM 6 electron microscope. The progress of this study was greatly facilitated by the excellent assistance of Mr T. Davies and Miss C. Green. The stereoscan micrograph (P1. 1, fig. 2) is published by permission of the Cambridge Instrument Company.


Sign in / Sign up

Export Citation Format

Share Document