Developmental changes in Ca2+ currents from newborn rat cardiomyocytes in primary culture

1994 ◽  
Vol 428 (3-4) ◽  
pp. 241-249 ◽  
Author(s):  
Jean -Pierre Gomez ◽  
Daniel Potreau ◽  
Jean -Eric Branka ◽  
Guy Raymond
Keyword(s):  
Primary Culture ◽  
Developmental Changes ◽  
Newborn Rat ◽  
Rat Cardiomyocytes

scholarly journals In Vitro Effects of Yessotoxin on a Primary Culture of Rat Cardiomyocytes

2008 ◽  
Vol 106 (2) ◽  
pp. 392-399 ◽  
Author(s):  
Valeria Dell'Ovo ◽  
Elena Bandi ◽  
Tamara Coslovich ◽  
Chiara Florio ◽  
Marina Sciancalepore ◽  
...  
Keyword(s):  
Primary Culture ◽  
Rat Cardiomyocytes ◽  
In Vitro Effects

Nifedipine-activated Ca2+ permeability in newborn rat cortical collecting duct cells in primary culture

2001 ◽  
Vol 280 (5) ◽  
pp. C1193-C1203 ◽  
Author(s):  
Laura Valencia ◽  
Michel Bidet ◽  
Sonia Martial ◽  
Elsa Sanchez ◽  
Estela Melendez ◽  
...  
Keyword(s):  
Primary Culture ◽  
Transient Receptor Potential ◽  
Collecting Duct ◽  
Primary Cultures ◽  
Receptor Potential ◽  
Cortical Collecting Duct ◽  
Newborn Rat ◽  
Adult Rat ◽  
Intracellular Ca ◽  
Transient Receptor

To characterize Ca2+ transport in newborn rat cortical collecting duct (CCD) cells, we used nifedipine, which in adult rat distal tubules inhibits the intracellular Ca2+concentration ([Ca2+]i) increase in response to hormonal activation. We found that the dihydropyridine (DHP) nifedipine (20 μM) produced an increase in [Ca2+]i from 87.6 ± 3.3 nM to 389.9 ± 29.0 nM in 65% of the cells. Similar effects of other DHP (BAY K 8644, isradipine) were also observed. Conversely, DHPs did not induce any increase in [Ca2+]i in cells obtained from proximal convoluted tubule. In CCD cells, neither verapamil nor diltiazem induced any rise in [Ca2+]i. Experiments in the presence of EGTA showed that external Ca2+ was required for the nifedipine effect, while lanthanum (20 μM), gadolinium (100 μM), and diltiazem (20 μM) inhibited the effect. Experiments done in the presence of valinomycin resulted in the same nifedipine effect, showing that K+ channels were not involved in the nifedipine-induced [Ca2+]i rise. H2O2also triggered [Ca2+]i rise. However, nifedipine-induced [Ca2+]i increase was not affected by protamine. In conclusion, the present results indicate that 1) primary cultures of cells from terminal nephron of newborn rats are a useful tool for investigating Ca2+transport mechanisms during growth, and 2) newborn rat CCD cells in primary culture exhibit a new apical nifedipine-activated Ca2+ channel of capacitive type (either transient receptor potential or leak channel).

Hypotonically induced calcium increase and regulatory volume decrease in newborn rat cardiomyocytes

1998 ◽  
Vol 436 (4) ◽  
pp. 565-574 ◽  
Author(s):  
Karima Taouil ◽  
Raffaella Giancola ◽  
Jean-Emile Morel ◽  
P. Hannaert
Keyword(s):  
Regulatory Volume Decrease ◽  
Newborn Rat ◽  
Rat Cardiomyocytes ◽  
Volume Decrease
Sign in / Sign up

Export Citation Format

Share Document