Cytochrome P-450 monooxygenase, epoxide hydrolase and flavin monooxygenase activities in clara cells and alveolar type II cells isolated from rabbit

1985 ◽  
Vol 1 (2) ◽  
pp. 57-65 ◽  
Author(s):  
Theodora R. Devereux ◽  
Janet J. Diliberto ◽  
James R. Fouts
Keyword(s):  
Epoxide Hydrolase ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Clara Cells ◽  
Alveolar Type Ii Cells ◽  
Flavin Monooxygenase ◽  
Cytochrome P 450

Differential capacities for DNA repair in Clara cells, alveolar type II cells and macrophages of rabbit lung

1985 ◽  
Vol 6 (4) ◽  
pp. 661-663 ◽  
Author(s):  
Toralf Deilhaug ◽  
Bjørnar Myrnes ◽  
Tore Aune ◽  
Hans Krokan ◽  
Aage Haugen
Keyword(s):  
Dna Repair ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Clara Cells ◽  
Alveolar Type Ii Cells ◽  
Rabbit Lung

scholarly journals Surfactant Protein B (SP-B) −/− Mice Are Rescued by Restoration of SP-B Expression in Alveolar Type II Cells but Not Clara Cells

1999 ◽  
Vol 274 (27) ◽  
pp. 19168-19174 ◽  
Author(s):  
Sui Lin ◽  
Cheng-Lun Na ◽  
Henry T. Akinbi ◽  
Karen S. Apsley ◽  
Jeffrey A. Whitsett ◽  
...  
Keyword(s):  
Surfactant Protein ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Clara Cells ◽  
Alveolar Type Ii Cells ◽  
Surfactant Protein B ◽  
B Mice ◽  
Protein B

Identification of Cytochrome P-450 Isozymes in Nonciliated Bronchiolar Epithelial (Clara) and Alveolar Type II Cells Isolated from Rabbit Lung

1981 ◽  
Vol 2 (3) ◽  
pp. 221-230 ◽  
Author(s):  
Theodora R. Devereux ◽  
Cosette J. Serabjit-singh ◽  
Shelley R. Slaughter ◽  
C. Roland Wolf ◽  
Richard M. Philpot ◽  
...  
Keyword(s):  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Rabbit Lung ◽  
Cytochrome P 450

Isolation of nonciliated bronchiolar epithelial (Clara) cells and alveolar type II cells from mouse lungs

1987 ◽  
Vol 65 (12) ◽  
pp. 2368-2372 ◽  
Author(s):  
Thomas E. Massey ◽  
Barbara A. Geddes ◽  
Poh-Gek Forkert
Keyword(s):  
Viable Cell ◽  
Alveolar Type ◽  
Type I ◽  
Type Ii Cells ◽  
Type Ii ◽  
Clara Cells ◽  
Isolated Cells ◽  
Alveolar Type Ii Cells ◽  
Trypan Blue Exclusion ◽  
Mouse Lungs

A method is described for the isolation of alveolar type II cells and nonciliated bronchiolar epithelial (Clara) cells from mouse lungs. Following digestion of lung tissue with Sigma type I protease, viable cells were isolated to 65% purity for type II cells (6.4 ± 1.5 × 105 cells/mouse) and 55–60% purity for Clara cells (2.6 ± 0.9 × 105 cells/mouse). Viability, as assessed by trypan blue exclusion, was routinely greater than 90% in all enriched cell fractions. Although minor mitochondrial changes occurred during isolation, the morphology of the cells showed good preservation, as revealed by electron microscopy. The isolated cells were found to be metabolically active, as indicated by the presence of 7-ethoxycoumarin deethylase (a cytochrome p-450-mediated activity). The highest activity of this enzyme (278 ± 116 pmol∙min−1∙mg protein−1) was found in the fraction enriched in Clara cells. The results indicate that this method produces viable cell populations that can be of value in investigations of the cellular distribution of lung metabolism activities.

Giant Lamellar Bodies in Alveolar Type II Cells of Rats Exposed to a Low Concentration of Ozone

Respiration ◽  
1984 ◽  
Vol 46 (3) ◽  
pp. 303-309 ◽  
Author(s):  
Sanae Shimura ◽  
Shinsaku Maeda ◽  
Tamotsu Takismima
Keyword(s):  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Lamellar Bodies ◽  
Alveolar Type Ii Cells ◽  
Low Concentration
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