Capillary endothelial cell migration: loss of stress fibres in response to retina-derived growth factor

1984 ◽  
Vol 5 (6) ◽  
pp. 697-709 ◽  
Author(s):  
Ira M. Herman ◽  
Patricia A. D'Amore
Keyword(s):  
Cell Migration ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Endothelial Cell Migration ◽  
Capillary Endothelial Cell ◽  
Migration Loss

scholarly journals A Central Role for Nicotinic Cholinergic Regulation of Growth Factor–Induced Endothelial Cell Migration

2007 ◽  
Vol 27 (1) ◽  
pp. 106-112 ◽  
Author(s):  
Martin K.C. Ng ◽  
Jenny Wu ◽  
Edwin Chang ◽  
Bing-yin Wang ◽  
Regina Katzenberg-Clark ◽  
...  
Keyword(s):  
Cell Migration ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Endothelial Cell Migration ◽  
Cholinergic Regulation ◽  
Regulation Of Growth

scholarly journals Redox Regulation of SERCA2 Is Required for Vascular Endothelial Growth Factor-Induced Signaling and Endothelial Cell Migration

2012 ◽  
Vol 17 (8) ◽  
pp. 1099-1108 ◽  
Author(s):  
Alicia M. Evangelista ◽  
Melissa D. Thompson ◽  
Robert M. Weisbrod ◽  
David R. Pimental ◽  
XiaoYong Tong ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Cell Migration ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Redox Regulation ◽  
Endothelial Cell Migration ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

Eicosanoid regulation of angiogenesis: role of endothelial arachidonate 12-lipoxygenase

Blood ◽  
2000 ◽  
Vol 95 (7) ◽  
pp. 2304-2311
Author(s):  
Daotai Nie ◽  
Keqin Tang ◽  
Clement Diglio ◽  
Kenneth V. Honn
Keyword(s):  
Endothelial Cells ◽  
Cell Migration ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Cell Line ◽  
Critical Role ◽  
Endothelial Cell Migration ◽  
Vascular Endothelial ◽  
12 Lipoxygenase

Angiogenesis, the formation of new capillaries from preexisting blood vessels, is a multistep, highly orchestrated process involving vessel sprouting, endothelial cell migration, proliferation, tube differentiation, and survival. Eicosanoids, arachidonic acid (AA)-derived metabolites, have potent biologic activities on vascular endothelial cells. Endothelial cells can synthesize various eicosanoids, including the 12-lipoxygenase (LOX) product 12(S)-hydroxyeicosatetraenoic acid (HETE). Here we demonstrate that endogenous 12-LOX is involved in endothelial cell angiogenic responses. First, the 12-LOX inhibitor, N-benzyl-N-hydroxy-5-phenylpentanamide (BHPP), reduced endothelial cell proliferation stimulated either by basic fibroblast growth factor (bFGF) or by vascular endothelial growth factor (VEGF). Second, 12-LOX inhibitors blocked VEGF-induced endothelial cell migration, and this blockage could be partially reversed by the addition of 12(S)-HETE. Third, pretreatment of an angiogenic endothelial cell line, RV-ECT, with BHPP significantly inhibited the formation of tubelike/cordlike structures within Matrigel. Fourth, overexpression of 12-LOX in the CD4 endothelial cell line significantly stimulated cell migration and tube differentiation. In agreement with the critical role of 12-LOX in endothelial cell angiogenic responses in vitro, the 12-LOX inhibitor BHPP significantly reduced bFGF-induced angiogenesis in vivo using a Matrigel implantation bioassay. These findings demonstrate that AA metabolism in endothelial cells, especially the 12-LOX pathway, plays a critical role in angiogenesis.

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