scholarly journals Nerve growth factor: Cellular localization and regulation of synthesis

1988 ◽  
Vol 8 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Hans Thoenen ◽  
Christine Bandtlow ◽  
Rolf Heumann ◽  
Dan Lindholm ◽  
Michael Meyer ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cellular Localization ◽  
Nerve Growth ◽  
Regulation Of Synthesis

scholarly journals The nuclear, 75 kDa form of early growth response protein-1/nerve growth factor-induced A protein is primarily restricted to LH beta-subunit-expressing cells in rat anterior pituitary

2000 ◽  
pp. 817-821 ◽  
Author(s):  
C Knight ◽  
JP Slade ◽  
D Carter
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Anterior Pituitary ◽  
Cellular Localization ◽  
Growth Response ◽  
Early Growth ◽  
Beta Subunit ◽  
Immunocytochemical Localization ◽  
Nerve Growth ◽  
Early Growth Response

OBJECTIVE: Early growth response protein-1 (Egr-1)/nerve growth factor-induced A (also known as Zif-268, Krox-24, TIS8, ZENK) is a zinc-finger transcription factor which, although expressed in a range of organ systems, has been shown to be essential only in the maintenance of fertility through actions within the endocrine system. In the present study we have investigated the anatomical basis for actions of Egr-1 in the adult anterior pituitary gland. DESIGN: Using female rats and mice as experimental models, we have used immunocytochemical and microscopic analysis to make observations of the cellular and sub-cellular localization of Egr-1 protein. METHODS: Immuno-(Western) blotting was first used to characterize the anterior pituitary proteins detected by a commercially available Egr-1 antibody. Subsequently, the antibody was used both singly and in combination with an LH beta-subunit antibody, for immunocytochemical localization studies in pituitary sections. RESULTS: The Egr-1 antiserum detected a single, major (primarily nuclear) 75 kDa protein band in Western blots of anterior pituitary extracts. Nuclear localization was confirmed by immunocytochemistry, which also demonstrated that the 75 kDa protein is localized to a minority sub-population of anterior pituitary cells in both rat and mouse. Dual immunocytochemical localization showed that the Egr-1 protein is primarily restricted to the nuclei of LH beta-subunit-expressing cells. CONCLUSIONS: Sub-cellular localization of Egr-1 to the nucleus is consistent with a direct role in transcriptional regulation of anterior pituitary function in the adult rat. Furthermore, a selective role in the physiological control of gonadotrophin gene expression is indicated.

Screening Natural Products for Effects on Nerve Growth Factor (NGF) Stimulated Neuritogenesis and Neuritic Outgrowth

Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
ND Chaurasiya ◽  
R Sahu ◽  
V Samoylenko ◽  
M Ilias ◽  
LA Walker ◽  
...  
Keyword(s):  
Natural Products ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth ◽  
Neuritic Outgrowth

Nerve Growth Factor overexpression in Clara cells suppresses metastasis and tumor growth in a mouse model of experimental lung metastasis

Pneumologie ◽  
2012 ◽  
Vol 66 (06) ◽  
Author(s):  
K Seidler ◽  
A Sydykov ◽  
S Müller-Brüsselbach ◽  
R Müller ◽  
N Weißmann ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mouse Model ◽  
Tumor Growth ◽  
Lung Metastasis ◽  
Clara Cells ◽  
Nerve Growth
Sign in / Sign up

Export Citation Format

Share Document