Effects of oxygen concentration on embryonic development in rats: a light and electron microscopic study using whole-embryo culture techniques

1988 ◽  
Vol 178 (4) ◽  
pp. 337-343 ◽  
Author(s):  
A. Miki ◽  
E. Fujimoto ◽  
T. Ohsaki ◽  
H. Mizoguti
Keyword(s):  
Embryonic Development ◽  
Microscopic Study ◽  
Oxygen Concentration ◽  
Electron Microscopic Study ◽  
Embryo Culture ◽  
Electron Microscopic ◽  
Whole Embryo Culture ◽  
Culture Techniques ◽  
Embryo Culture Techniques

scholarly journals Changing intracellular compartmentalization of ?-galactosidase in the ROSA26 reporter mouse during embryonic development: A light- and electron-microscopic study

2004 ◽  
Vol 279A (2) ◽  
pp. 740-748 ◽  
Author(s):  
Naoyoshi Aoyama ◽  
Daniel G.M. Molin ◽  
Monica M.T. Mentink ◽  
Henk K. Koerten ◽  
Marco C. de Ruiter ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Electron Microscopic ◽  
Reporter Mouse ◽  
Intracellular Compartmentalization

Induction of cleft lip in cultured rat embryos by localized administration of tunicamycin

Development ◽  
1981 ◽  
Vol 64 (1) ◽  
pp. 1-9
Author(s):  
Kazuhiro Eto ◽  
Alvaro Figueroa ◽  
Gakuzo Tamura ◽  
Robert M. Pratt
Keyword(s):  
Embryo Culture ◽  
Cleft Lip ◽  
Protein Glycosylation ◽  
Whole Embryo Culture ◽  
Rat Embryos ◽  
Culture Techniques ◽  
Primary Palate ◽  
Development In Vitro ◽  
Embryo Culture Techniques

Whole-embryo culture techniques have advanced to the point where the study of normal and abnormal primary palate development in vitro is possible. The purpose of this study was to ascertain whether localized administration of tunicamycin (TM) an inhibitor of protein glycosylation, into the region of the developing primary palate would induce cleft lip in culture. Rat embryos were explanted on late day 11 of gestation and cultured with open yolk sacs for 40 h. TM was administered by implanting a sterile eyelash soaked in TM into the nasal placode region. The contralateral placode was used as the control by implanting an eyelash lacking TM. Under these conditions, TM-treated placodes were found to develop cleft lip in 14 out of 15 embryos compared to 0 for the controls. These experiments demonstrate that localized administration of TM results in cleft lip formation in whole embryo culture. The technique of localized administration of drugs and teratogens in whole embryo culture should prove useful for similar studies on embryonic development.

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