Effects of several lipidosis-inducing drugs upon the area postrema and adjacent medullary nuclei of adult rats

1981 ◽  
Vol 53 (1) ◽  
pp. 41-50 ◽  
Author(s):  
W. Frisch ◽  
Renate L�llmann-Rauch
Keyword(s):  
Area Postrema ◽  
Adult Rats ◽  
Medullary Nuclei

scholarly journals Peripheral-to-central immune communication at the area postrema glial-barrier following bleomycin-induced sterile lung injury in adult rats

2020 ◽  
Vol 87 ◽  
pp. 610-633 ◽  
Author(s):  
David G. Litvin ◽  
Scott J. Denstaedt ◽  
Lauren F. Borkowski ◽  
Nicole L. Nichols ◽  
Thomas E. Dick ◽  
...  
Keyword(s):  
Lung Injury ◽  
Area Postrema ◽  
Adult Rats ◽  
Glial Barrier

scholarly journals The satiating hormone amylin enhances neurogenesis in the area postrema of adult rats

2016 ◽  
Vol 5 (10) ◽  
pp. 834-843 ◽  
Author(s):  
Claudia G. Liberini ◽  
Tito Borner ◽  
Christina N. Boyle ◽  
Thomas A. Lutz
Keyword(s):  
Area Postrema ◽  
Adult Rats

scholarly journals Endogenous amylin contributes to birth of microglial cells in arcuate nucleus of hypothalamus and area postrema during fetal development

2019 ◽  
Vol 316 (6) ◽  
pp. R791-R801 ◽  
Author(s):  
Thomas A. Lutz ◽  
Christelle Le Foll
Keyword(s):  
Fetal Development ◽  
Arcuate Nucleus ◽  
Nucleus Tractus Solitarius ◽  
Area Postrema ◽  
Critical Factor ◽  
Microglial Cells ◽  
Dorsomedial Nucleus ◽  
Adult Rats ◽  
Axonal Fiber ◽  
Control Food Intake

Amylin acts in the area postrema (AP) and arcuate nucleus (ARC) to control food intake. Amylin also increases axonal fiber outgrowth from the AP→nucleus tractus solitarius and from ARC→hypothalamic paraventricular nucleus. More recently, exogenous amylin infusion for 4 wk was shown to increase neurogenesis in adult rats in the AP. Furthermore, amylin has been shown to enhance leptin signaling in the ARC and ventromedial nucleus of the hypothalamus (VMN). Thus, we hypothesized that endogenous amylin could be a critical factor in regulating cell birth in the ARC and AP and that amylin could also be involved in the birth of leptin-sensitive neurons. Amylin+/− dams were injected with BrdU at embryonic day 12 and at postnatal day 2; BrdU+ cells were quantified in wild-type (WT) and amylin knockout (KO) mice. The number of BrdU+HuC/D+ neurons was similar in ARC and AP, but the number of BrdU+Iba1+ microglia was significantly decreased in both nuclei. Five-week-old WT and KO littermates were injected with leptin to test whether amylin is involved in the birth of leptin-sensitive neurons. Although there was no difference in the number of BrdU+c-Fos+ neurons in the ARC and dorsomedial nucleus, an increase in BrdU+c-Fos+ neurons was seen in VMN and lateral hypothalamus (LH) in amylin KO mice. In conclusion, these data suggest that during fetal development, endogenous amylin favors the birth of microglial cells in the ARC and AP and that it decreases the birth of leptin-sensitive neurons in the VMN and LH.

Further evidence that the blood/brain barrier impedes paraquat entry into the brain

1995 ◽  
Vol 14 (7) ◽  
pp. 587-594 ◽  
Author(s):  
JL Naylor ◽  
PS Widdowson ◽  
MG Simpson ◽  
M. Farnworth ◽  
MK Ellis ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Area Postrema ◽  
Neuronal Cell ◽  
Brain Regions ◽  
Systemic Administration ◽  
Brain Barrier ◽  
Adult Rats ◽  
Male Adult ◽  
Blood Brain ◽  
The Brain

The distribution of the non-selective herbicide paraquat was examined in the brain following subcutaneous admin istration of 20 mg kg -1 paraquat ion containing [14C]paraquat to male adult rats in order to determine whether paraquat crosses the blood/brain barrier. Following administration, [14C]paraquat reached a maxi mal concentration in the brain (0.05% of administered dose) within the first hour and then rapidly disappeared from the brain. However, 24 h after administration of the herbicide, about 13% of the maximal recorded concentra tion of paraquat remained in the brain (1.6 nmol g-1 wet weight) and could not be removed by intracardiac perfu sion. Using measurements of [14C]paraquat in dissected brain regions and using quantitative autoradiography we demonstrated an asymmetrical distribution in and around the brain at 30 min (maximal concentration) and 24 h after administration. Most of the paraquat was associated with five structures, two of which, the pineal gland and linings of the cerebral ventricles lie outside the blood/brain barrier whilst the remaining three brain areas, the anterior portion of the olfactory bulb, hypothalamus and area postrema do not have a blood/brain barrier. Overall, the distribution of [14C]paraquat in the brain 24 h after systemic administration was highly correlated to the blood volume. These data indicate that any remaining paraquat in the brain 24 h after systemic administration is associated with elements of the cerebro-circulatory sys tem, such as the endothelial cells that make up the capil lary network and that there is a limited entry of paraquat into brain regions without a blood/brain barrier. No [14C]paraquat was detected in regions where there has been demonstrated pathology in brains from humans with Parkinson's disease. Finally, we could find no evidence for paraquat-induced neuronal cell necrosis 24 or 48 h after systemic administration. Overall it may be concluded that systemically administered paraquat does not pose a direct major neurotoxicological risk in the majority of brain regions which have a functional blood/brain barrier since paraquat can be excluded from the brain by this barrier.

Further Studies on the Ultrastructure of Harderian Gland in Adult Rats

1974 ◽  
Vol 32 ◽  
pp. 288-289
Author(s):  
Alfredo Feria-Velasco ◽  
Guadalupe Tapia-Arizmendi
Keyword(s):  
Fine Structure ◽  
Domestic Fowl ◽  
Animal Species ◽  
Acinar Cells ◽  
Harderian Gland ◽  
Myoepithelial Cells ◽  
The Other ◽  
Albino Rats ◽  
Adult Rats ◽  
Cell Components

The fine structure of the Harderian gland has been described in some animal species (hamster, rabbit, mouse, domestic fowl and albino rats). There are only two reports in the literature dealing on the ultrastructure of rat Harderian gland in adult animals. In one of them the author describes the myoepithelial cells in methacrylate-embbeded tissue, and the other deals with the maturation of the acinar cells and the formation of the secretory droplets. The aim of the present work is to analize the relationships among the acinar cell components and to describe the two types of cells located at the perifery of the acini.

Permeability of the microcirculation in area postrema of rat

1988 ◽  
Vol 46 ◽  
pp. 348-349
Author(s):  
Shams M. Ghoneim ◽  
Frank M. Faraci ◽  
Gary L. Baumbach
Keyword(s):  
Brain Stem ◽  
Area Postrema ◽  
Upper Body ◽  
Sprague Dawley ◽  
Sodium Cacodylate ◽  
Acute Hypertension ◽  
Sprague Dawley Rats ◽  
Ultrastructural Characteristics ◽  
The Brain ◽  
Adjacent Brain

The area postrema is a circumventricular organ in the brain stem and is one of the regions in the brain that lacks a fully functional blood-brain barrier. Recently, we found that disruption of the microcirculation during acute hypertension is greater in area postrema than in the adjacent brain stem. In contrast, hyperosmolar disruption of the microcirculation is greater in brain stem. The objective of this study was to compare ultrastructural characteristics of the microcirculation in area postrema and adjacent brain stem.We studied 5 Sprague-Dawley rats. Horseradish peroxidase was injected intravenously and allowed to circulate for 1, 5 or 15 minutes. Following perfusion of the upper body with 2.25% glutaraldehyde in 0.1 M sodium cacodylate, the brain stem was removed, embedded in agar, and chopped into 50-70 μm sections with a TC-Sorvall tissue chopper. Sections of brain stem were incubated for 1 hour in a solution of 3,3' diaminobenzidine tetrahydrochloride (0.05%) in 0.05M Tris buffer with 1% H2O2.

Biodegradable polyester neuroprostheses promote the reconnection of separated peripheral nerve stubs

1992 ◽  
Vol 50 (2) ◽  
pp. 1094-1095
Author(s):  
Beverly L. Giammara ◽  
Jennifer S. Stevenson ◽  
Peggy E. Yates ◽  
Robert H. Gunderson ◽  
Jacob S. Hanker
Keyword(s):  
Image Analysis ◽  
Basement Membrane ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Computer Assisted ◽  
Type Iii Collagen ◽  
Image Analysis System ◽  
Biodegradable Polyester ◽  
Adult Rats ◽  
Type Iv

An 11mm length of sciatic nerve was removed from 10 anesthetized adult rats and replaced by a biodegradable polyester Vicryl™ mesh sleeve which was then injected with the basement membrane gel, Matrigel™. It was noted that leg sensation and movement were much improved after 30 to 45 days and upon sacrifice nerve reconnection was noted in all animals. Epoxy sections of the repaired nerves were compared with those of the excised segments by the use of a variation of the PAS reaction, the PATS reaction, developed in our laboratories for light and electron microscopy. This microwave-accelerated technique employs periodic acid, thiocarbohydrazide and silver methenamine. It stains basement membrane or Type IV collagen brown and type III collagen (reticulin), axons, Schwann cells, endoneurium and perineurium black. Epoxy sections of repaired and excised nerves were also compared by toluidine blue (tb) staining. Comparison of the sections of control and repaired nerves was done by computer-assisted microscopic image analysis using an Olympus CUE-2 Image Analysis System.

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