Effects of lithium on morphological characteristics of dissociated brain cells in culture

1979 ◽  
Vol 46 (1-2) ◽  
pp. 117-121 ◽  
Author(s):  
Z. Janka ◽  
I. Szentistv�nyi ◽  
F. Joo ◽  
A. Juh�sz ◽  
�. Riman�czy
Keyword(s):  
Morphological Characteristics ◽  
Brain Cells ◽  
Cells In Culture ◽  
Dissociated Brain Cells

Down-regulation of free intracellular calcium in dissociated brain cells of aged mice and rats

Life Sciences ◽  
1996 ◽  
Vol 59 (5-6) ◽  
pp. 435-449 ◽  
Author(s):  
Henrike Hartmann ◽  
Anne Eckert ◽  
Karsten Velbinger ◽  
Michael Rewsin ◽  
Walter E. Müller
Keyword(s):  
Intracellular Calcium ◽  
Brain Cells ◽  
Down Regulation ◽  
Aged Mice ◽  
Free Intracellular Calcium ◽  
Dissociated Brain Cells

Dexamethasone-Induced Accumulation of Neuropeptide-Y by Aggregating Fetal Brain Cells in Culture: A Process Dependent on the Developmental Age of the Aggregates*

Endocrinology ◽  
1991 ◽  
Vol 129 (2) ◽  
pp. 931-938 ◽  
Author(s):  
AYALLA BARNEA ◽  
GLORIA CHO ◽  
ASGHAR HAJIBEIGI ◽  
M. CECILIA AGUILA ◽  
PAOLO MAGNI
Keyword(s):  
Neuropeptide Y ◽  
Fetal Brain ◽  
Brain Cells ◽  
Cells In Culture ◽  
Developmental Age

scholarly journals Immunoreactive insulin from mouse brain cells in culture and whole rat brain

1984 ◽  
Vol 218 (1) ◽  
pp. 19-27 ◽  
Author(s):  
N P Birch ◽  
D L Christie ◽  
A G C Renwick
Keyword(s):  
Rat Brain ◽  
Mouse Brain ◽  
Culture Medium ◽  
Calf Serum ◽  
Brain Cells ◽  
Immunoreactive Insulin ◽  
Foetal Calf Serum ◽  
Cells In Culture ◽  
Pancreatic Insulin ◽  
Contrast Microscopy

Foetal mouse brain cells were cultured as described previously [Sotelo, Gibbs, Gajdusek, Toh & Wurth (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 653-657] without added insulin and without foetal calf serum after 12 days in culture. Examination by phase-contrast microscopy showed that these modifications did not appear to affect growth and development of the cells adversely. Silver impregnation of the cultures and indirect immunofluorescence following reaction with tetanus toxin showed that a high proportion of the cells resembled neurones. Analysis of concentrated culture medium by radioimmunoassay and high-pressure liquid chromatography (h.p.l.c.) revealed that the cells produced two main forms of immunoreactive insulin which differed from authentic pancreatic insulin in retention time. Immunoreactive somatostatin was also produced in culture and this was resolved into at least three forms by h.p.l.c. Immunoreactive insulin was also extracted from whole rat brain by using two published procedures. The method of Havrankova, Schmechel, Roth & Brownstein [Proc. Natl. Acad. Sci. U.S.A. (1978) 75, 5737-5741] consistently gave greater yields of insulin than did that of Eng & Yalow [Diabetes (1980) 29, 105-109] and the concentration was about three times that of plasma. The extracted insulin was further characterized by h.p.l.c. in each case and was found to behave like authentic pancreatic insulin. The production of insulin and somatostatin by foetal mouse brain cells in culture suggests that they may be a useful model system for studies of neuropeptide biosynthesis.

Steady-state distribution of lithium during cultivation of dissociated brain cells

1980 ◽  
Vol 36 (9) ◽  
pp. 1071-1072 ◽  
Author(s):  
Z. Janka ◽  
I. Szentistványi ◽  
A. Juhász ◽  
A. Rimanóczy
Keyword(s):  
Steady State ◽  
Brain Cells ◽  
Steady State Distribution ◽  
State Distribution ◽  
Dissociated Brain Cells
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