Extravasation, spread and cellular uptake of Evans blue-labelled albumin around a reproducible small stab-wound in the rat brain

1976 ◽  
Vol 34 (2) ◽  
pp. 125-136 ◽  
Author(s):  
L. Persson ◽  
H. -A. Hansson ◽  
P. Sourander
Keyword(s):  
Rat Brain ◽  
Cellular Uptake ◽  
Evans Blue ◽  
Stab Wound

Capillary perfusion during incomplete forebrain ischemia and reperfusion in rat brain

1993 ◽  
Vol 265 (2) ◽  
pp. H642-H648 ◽  
Author(s):  
H. Theilen ◽  
H. Schrock ◽  
W. Kuschinsky
Keyword(s):  
Rat Brain ◽  
Evans Blue ◽  
Ischemia Reperfusion ◽  
Experimental Models ◽  
Forebrain Ischemia ◽  
Capillary Perfusion ◽  
Experimental Conditions ◽  
Brain Capillary ◽  
Arterial Blood ◽  
The Brain

Previous studies have shown a complete plasma perfusion of all capillaries in the rat brain under normal physiological conditions. This raises the question under which experimental conditions nonperfused capillaries may show up in the brain. Two experimental models were investigated in rats. 1) Reduced cerebral blood flow (CBF) during incomplete forebrain ischemia: hemorrhagic hypotension was maintained for 30 min at a mean arterial blood pressure of 41 mmHg. During the final 5 min of hypotension both carotid arteries were ligated. 2) Reperfusion after incomplete forebrain ischemia: reperfusion lasted for 4 h after either 15 or 30 min of incomplete forebrain ischemia. Under both experimental conditions, the density of the existing as well as the plasma-perfused brain capillary network was quantified using fluorescent double staining. Local CBF was measured during incomplete forebrain ischemia using the quantitative autoradiographic 4-iodo-[N-methyl-14C]antipyrine technique. The results showed a decrease in CBF during incomplete forebrain ischemia, which amounted up to 94%. Whereas normotensive control animals showed a complete staining of all capillaries within 5 s after the intravenous injection of Evans blue, this period of time was increased to 10 s during incomplete forebrain ischemia, indicating a delayed capillary perfusion. Four hours of reperfusion after 15 min of incomplete forebrain ischemia resulted in a complete capillary staining, whereas reperfusion after 30 min of ischemia was followed by intracerebral bleedings and a few nonperfused capillary areas (circulation time of Evans blue: 10 s).(ABSTRACT TRUNCATED AT 250 WORDS)

Glycogen reaction to cerebral stab wound during maturation of rat brain

1972 ◽  
Vol 22 (2) ◽  
pp. 158-169 ◽  
Author(s):  
Edith Farkas-Bargeton ◽  
Yngve Olsson ◽  
Lloyd Guth ◽  
Igor Klatzo
Keyword(s):  
Rat Brain ◽  
Stab Wound ◽  
Glycogen Reaction

scholarly journals Reduction of Ischemic Damage by Application of Vascular Endothelial Growth Factor in Rat Brain after Transient Ischemia

1998 ◽  
Vol 18 (8) ◽  
pp. 887-895 ◽  
Author(s):  
Takeshi Hayashi ◽  
Koji Abe ◽  
Yasuto Itoyama
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Rat Brain ◽  
Evans Blue ◽  
Infarct Volume ◽  
Vascular Endothelial ◽  
Transient Ischemia ◽  
Edema Formation ◽  
Ischemic Brain ◽  
Endothelial Growth Factor

Vascular endothelial growth factor (VEGF) is a secreted polypeptide and plays a pivotal role in angiogenesis in vivo. However, it also increases vascular permeability, and might exacerbate ischemic brain edema. The effect of this factor on the brain after transient ischemia was investigated in terms of infarct volume and edema formation, as well as cellular injury. After 90 minutes of transient middle cerebral artery occlusion, VEGF (1.0 ng/μL, 9 μL) was topically applied on the surface of the reperfused rat brain. A significant reduction of infarct volume was found in animals with VEGF application ( P < 0.001) at 24 hours of reperfusion as compared with cases with vehicle treatment. Brain edema was significantly reduced in VEGF-treated animals ( P = 0.01), and furthermore, extravasation of Evans blue was also decreased in those animals ( P < 0.01). Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling and immunohistochemical analysis for 70-kDa heat shock protein showed an amelioration of the stainings at 24 and 48 hours after reperfusion with VEGF treatment, which indicated reduction of neuronal damage. These results indicate that treatment with topical VEGF application significantly reduces ischemic brain damage, such as infarct volume, edema formation, and extravasation of Evans blue, and that the reductions were associated with that of neuronal injury.

BQ788, an endothelin ETB receptor antagonist, attenuates stab wound injury-induced reactive astrocytes in rat brain

Glia ◽  
1999 ◽  
Vol 26 (3) ◽  
pp. 268-271 ◽  
Author(s):  
Yutaka Koyama ◽  
Motohide Takemura ◽  
Keiko Fujiki ◽  
Nobue Ishikawa ◽  
Yoshio Shigenaga ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Rat Brain ◽  
Stab Wound ◽  
Reactive Astrocytes ◽  
Etb Receptor

Cellular uptake and spread of the cell-permeable peptide penetratin in adult rat brain

2000 ◽  
Vol 12 (8) ◽  
pp. 2847-2855 ◽  
Author(s):  
Sarah J. Bolton ◽  
Declan N. C. Jones ◽  
John G. Darker ◽  
Drake S. Eggleston ◽  
A. Jacqueline Hunter ◽  
...  
Keyword(s):  
Rat Brain ◽  
Cellular Uptake ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Cell Permeable Peptide

Water-soluble amphiphilic ruthenium(ii) polypyridyl complexes as potential light-activated therapeutic agents

2020 ◽  
Vol 56 (65) ◽  
pp. 9332-9335
Author(s):  
Sandra Estalayo-Adrián ◽  
Salvador Blasco ◽  
Sandra A. Bright ◽  
Gavin J. McManus ◽  
Guillermo Orellana ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Therapeutic Agents ◽  
Water Soluble ◽  
Polypyridyl Complexes ◽  
Cervical Cancer Cells

Two new water-soluble amphiphilic Ru(ii) polypyridyl complexes were synthesised and their photophysical and photobiological properties evaluated; both complexes showed a rapid cellular uptake and phototoxicity against HeLa cervical cancer cells.

cDNA cloning and expression of a gamma-aminobutyric acidA receptor epsilon-subunit in rat brain

2000 ◽  
Vol 12 (12) ◽  
pp. 4318-4330 ◽  
Author(s):  
Nathalie Moragues ◽  
Philippe Ciofi ◽  
Pierrette Lafon ◽  
Marie-Francoise Odessa ◽  
Gerard Tramu ◽  
...  
Keyword(s):  
Rat Brain ◽  
Cdna Cloning ◽  
Cloning And Expression ◽  
Epsilon Subunit

Antisense knockdown of the glial glutamate transporter GLT-1 exacerbates hippocampal neuronal damage following traumatic injury to rat brain

2001 ◽  
Vol 13 (1) ◽  
pp. 119-128 ◽  
Author(s):  
Vemuganti L. Raghavendra Rao ◽  
Aclan Dogan ◽  
Kellie K. Bowen ◽  
Kathryn G. Todd ◽  
Robert J. Dempsey
Keyword(s):  
Rat Brain ◽  
Neuronal Damage ◽  
Traumatic Injury ◽  
Glutamate Transporter ◽  
Glial Glutamate Transporter ◽  
Antisense Knockdown
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