A biochemical approach to the electrogenic potassium pump of insect sensilla: Potassium sensitive ATPases in the labellum of the fly

1982 ◽  
Vol 148 (3) ◽  
pp. 303-311 ◽  
Author(s):  
Helmut Wieczorek
Keyword(s):  
Insect Sensilla ◽  
Biochemical Approach

scholarly journals Activation of NF-κB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xin Zhou ◽  
Ludvig J. Backman ◽  
Patrik Danielson
Keyword(s):  
Wound Healing ◽  
Mitochondrial Dna ◽  
Scar Formation ◽  
Mitochondrial Rna ◽  
Peripheral Part ◽  
Double Stranded Rna ◽  
Corneal Wound ◽  
Biochemical Approach ◽  
Common Deletion ◽  
Underlying Mechanisms

AbstractScar formation as a result of corneal wound healing is a leading cause of blindness. It is a challenge to understand why scar formation is more likely to occur in the central part of the cornea as compared to the peripheral part. The purpose of this study was to unravel the underlying mechanisms. We applied RNA-seq to uncover the differences of expression profile in keratocytes in the central/peripheral part of the cornea. The relative quantity of mitochondrial RNA was measured by multiplex qPCR. The characterization of mitochondrial RNA in the cytoplasm was confirmed by immunofluoresence microscope and biochemical approach. Gene expression was analyzed by western blot and RT qPCR. We demonstrate that the occurrence of mitochondrial DNA common deletion is greater in keratocytes from the central cornea as compared to those of the peripheral part. The keratocytes with CD have elevated oxidative stress levels, which leads to the leakage of mitochondrial double-stranded RNA into the cytoplasm. The cytoplasmic mitochondrial double-stranded RNA is sensed by MDA5, which induces NF-κB activation. The NF-κB activation thereafter induces fibrosis-like extracellular matrix expressions and IL-8 mRNA transcription. These results provide a novel explanation of the different clinical outcome in different regions of the cornea during wound healing.

scholarly journals Global mapping of protein–metabolite interactions in Saccharomyces cerevisiae reveals that Ser-Leu dipeptide regulates phosphoglycerate kinase activity

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Marcin Luzarowski ◽  
Rubén Vicente ◽  
Andrei Kiselev ◽  
Mateusz Wagner ◽  
Dennis Schlossarek ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Small Molecules ◽  
Phosphoglycerate Kinase ◽  
Glycolytic Enzyme ◽  
Diauxic Shift ◽  
Cellular Processes ◽  
Targeted Analysis ◽  
Global Mapping ◽  
Biochemical Approach ◽  
User Friendly

AbstractProtein–metabolite interactions are of crucial importance for all cellular processes but remain understudied. Here, we applied a biochemical approach named PROMIS, to address the complexity of the protein–small molecule interactome in the model yeast Saccharomyces cerevisiae. By doing so, we provide a unique dataset, which can be queried for interactions between 74 small molecules and 3982 proteins using a user-friendly interface available at https://promis.mpimp-golm.mpg.de/yeastpmi/. By interpolating PROMIS with the list of predicted protein–metabolite interactions, we provided experimental validation for 225 binding events. Remarkably, of the 74 small molecules co-eluting with proteins, 36 were proteogenic dipeptides. Targeted analysis of a representative dipeptide, Ser-Leu, revealed numerous protein interactors comprising chaperones, proteasomal subunits, and metabolic enzymes. We could further demonstrate that Ser-Leu binding increases activity of a glycolytic enzyme phosphoglycerate kinase (Pgk1). Consistent with the binding analysis, Ser-Leu supplementation leads to the acute metabolic changes and delays timing of a diauxic shift. Supported by the dipeptide accumulation analysis our work attests to the role of Ser-Leu as a metabolic regulator at the interface of protein degradation and central metabolism.

Putative binding mode of Escherichia coli exopolyphosphatase and polyphosphates based on a hybrid in silico/biochemical approach

2016 ◽  
Vol 606 ◽  
pp. 64-72 ◽  
Author(s):  
Cristhian Boetsch ◽  
Daniel R. Aguayo-Villegas ◽  
Fernando D. Gonzalez-Nilo ◽  
Á. Teresita Lisa ◽  
Paola R. Beassoni
Keyword(s):  
Escherichia Coli ◽  
In Silico ◽  
Binding Mode ◽  
Biochemical Approach

scholarly journals O-Linked glycans control glycoprotein processing by antigen-presenting cells: a biochemical approach to the molecular aspects of MUC1 processing by dendritic cells

2003 ◽  
Vol 33 (12) ◽  
pp. 3242-3254 ◽  
Author(s):  
Franz-Georg Hanisch ◽  
Tilo Schwientek ◽  
Michael S. Von Bergwelt-Baildon ◽  
Joachim L. Schultze ◽  
Olivera Finn
Keyword(s):  
Dendritic Cells ◽  
Antigen Presenting Cells ◽  
Glycoprotein Processing ◽  
Biochemical Approach ◽  
Molecular Aspects ◽  
Antigen Presenting

The therapeutic potential of aqueous extract of Falcaria vulgaris in the treatment of fatty liver disease: a histopathological and biochemical approach

2018 ◽  
Vol 28 (4) ◽  
pp. 955-961 ◽  
Author(s):  
Samaneh Goorani ◽  
Mohsen Zhaleh ◽  
Mohammad Kazem Koohi ◽  
Niloofar Seydi ◽  
Khodabakhsh Rashidi ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Aqueous Extract ◽  
Fatty Liver Disease ◽  
Therapeutic Potential ◽  
Falcaria Vulgaris ◽  
Biochemical Approach

BLOOD ELEMENTS AT FOREIGN SURFACES: A BIOCHEMICAL APPROACH TO THE STUDY OF THE ADSORPTION OF PLASMA PROTEINS

1977 ◽  
Vol 283 (1 The Behavior) ◽  
pp. 37-49 ◽  
Author(s):  
Shaul Kochwa ◽  
Robert S. Litwak ◽  
Richard E. Rosenfield ◽  
Edward F. Leonard
Keyword(s):  
Plasma Proteins ◽  
Biochemical Approach ◽  
Blood Elements
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