Diversity of ultrastructure in different phenotypes of cultured microvessel endothelial cells isolated from bovine corpus luteum

1991 ◽  
Vol 266 (1) ◽  
pp. 37-49 ◽  
Author(s):  
K. Spanel-Borowski
Keyword(s):  
Endothelial Cells ◽  
Corpus Luteum ◽  
Bovine Corpus Luteum ◽  
Microvessel Endothelial Cells

Influence of Nitric Oxide on the Secretory Function of the Bovine Corpus Luteum: Dependence on Cell Composition and Cell-to-Cell Communication

2003 ◽  
Vol 228 (6) ◽  
pp. 741-748 ◽  
Author(s):  
Jerzy J. Jaroszewski ◽  
Dariusz J. Skarzynski ◽  
Robert M. Blair ◽  
William Hansel
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Corpus Luteum ◽  
Cell Contact ◽  
Secretory Function ◽  
No Donor ◽  
Luteal Cells ◽  
Cell Composition ◽  
Bovine Corpus Luteum ◽  
Nos Inhibitor

The objective of the present study was to investigate the role of cell-to-cell contact in the influence of nitric oxide (NO) on the secretory function of the bovine corpus luteum (CL). In Experiment 1, separate small luteal cells (SLC) or large (LLC) luteal cells were perfused with 100 μ M spermineNONOate, a NO donor, or with 100 μ M Nω-nitro-L-arginine methyl ester (L-NAME), a NO synthase (NOS) inhibitor; in Experiment 2, a mixture of LLC and SLC and endothelial cells was cultured and incubated with spermineNONOate or L-NAME; in Experiment 3, spermineNONOate was perfused into the CL (100 mg/4 hr) by a microdialysis system in vivo. Perfusion of isolated SLC and LLC with the NO donor or NOS inhibitor (Experiment 1) did not affect ( P > 0.05) secretion of progesterone (P4) or oxytocin (OT). L-NAME perfusion increased ( P < 0.05) leukotriene C4 (LTC4) secretion by both SLC and LLC cells. Treatment of mixtures of luteal cells with an NO donor (Experiment 2) significantly decreased ( P < 0.001) secretion of P4 and OT and increased ( P < 0.001) production of prostaglandin F2α (PGF2α) and LTC4. L-NAME stimulated ( P < 0.001) P4 secretion, but did not influence ( P > 0.05) OT, PGF2α or LTC4 production. Intraluteal administration (Experiment 3) of spermineNONOate increased ( P < 0.001) LTC4 and PGF2α, decreased OT, but did not change P4 levels in perfusate samples. These data indicate that cell-to-cell contact and cell composition play important roles in the response of bovine CL to treatment with NO donors or NOS inhibitors, and that paracrine mechanisms are required for the full secretory response of the CL in NO action. Endothelial cells appear to be required for the full secretory response of the CL to NO.

scholarly journals TAMANHO DA AMOSTRA PARA ESTUDO DA PROPORÇÃO VOLUMÉTRICA DOS CONSTITUINTES DO CORPO LÚTEO BOVINO

2002 ◽  
Vol 7 (2) ◽  
Author(s):  
M. M. NEVES ◽  
A. P. MARQUES JR.
Keyword(s):  
Endothelial Cells ◽  
Soybean Oil ◽  
Corpus Luteum ◽  
Cell Types ◽  
Luteal Cell ◽  
Cell Nuclei ◽  
Cell Cytoplasm ◽  
Bovine Corpus Luteum

O objetivo deste estudo foi estabelecer o número de campos histológicos necessários para quantificar os componentes celulares do corpo lúteo bovino. Para os parâmetros citoplasma das células luteínicas, núcleo das células luteínicas e fibroblasto são necessários 30 campos, enquanto que para células endoteliais e pericito são necessários 35, podendo-se padronizar a técnica em 35 campos e 875 pontos por corpo lúteo. Soybean oil replaced by acidulated soapstock in broiler diets Abstract The purpose of this study was to determine the number of fields necessary for the quantification of the cell types of the bovine corpus luteum. A total of 30 fields is necessary for the quantification of the luteal cell cytoplasm, luteal cell nuclei and fibroblast, and 35 fields for the quantification of endothelial cells and pericycles. For the analysis of both parameters is recommended the study of 35 fields and 875 points per corpus luteum.

Expression of Superoxide Dismutase in Bovine Corpus Luteum and Cultured Luteal Endothelial Cells.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 215-215
Author(s):  
Tomas J. Acosta ◽  
Hai V. Van ◽  
Yuji Nakagawa ◽  
Seung-Hyung Lee ◽  
Takuo Hojo ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Superoxide Dismutase ◽  
Corpus Luteum ◽  
Bovine Corpus Luteum

scholarly journals Cultured microvascular endothelial cells derived from the bovine corpus luteum possess NCAM-140

1992 ◽  
Vol 201 (2) ◽  
pp. 545-548 ◽  
Author(s):  
Artur Mayerhofer ◽  
Katharina Spanel-Borowski ◽  
Sara Watkins ◽  
Manfred Gratzl
Keyword(s):  
Endothelial Cells ◽  
Corpus Luteum ◽  
Microvascular Endothelial Cells ◽  
Bovine Corpus Luteum ◽  
Microvascular Endothelial
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