Effect of dibutyryl cyclic AMP on glucose transport in isolated brush border membrane vesicles from the rat kidney

1984 ◽  
Vol 400 (1) ◽  
pp. 109-111 ◽  
Author(s):  
Andrew M. Kahn ◽  
Deborah Steplock ◽  
Edward J. Weinman
Keyword(s):  
Cyclic Amp ◽  
Glucose Transport ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Kidney ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Dibutyryl Cyclic Amp

scholarly journals Effect of parathyrin on the transport properties of isolated renal brush-border vesicles

1978 ◽  
Vol 172 (1) ◽  
pp. 49-56 ◽  
Author(s):  
C Evers ◽  
H Murer ◽  
R Kinne
Keyword(s):  
Cyclic Amp ◽  
Transport Properties ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Phosphate Uptake ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Transport Systems ◽  
Precipitation Method ◽  
Dibutyryl Cyclic Amp

The transport properties of brush-border membrane vesicles isolated by a calcium-precipitation method from the renal cortex of normal and parathyrin (parathyroid hormone)-treated rats were studied by a rapid-filtration technique. Parathyrin elicited a dose-dependent decrease in the Na+-dependent phosphate uptake by the brush-border membrane vesicles, but the uptake of D-glucose, Na+ and mannitol was not affected. A maximum inhibition of 30% was observed after the application of 30 U.S.P. units intramuscularly 1 h before the animals were killed. Intravenous infusion of dibutyryl cyclic AMP (0.5-1.5 MG) also decreased the phosphate uptake by the brush-border vesicles. Both dibutyryl cyclic AMP and parathyrin were ineffective when added in vitro to brush-border membrane vesicles isolated from normal rats. These data suggest that parathyrin exerts its action on the phosphate reabsorption in the renal proximal tubule by affecting the Na+/phosphate co-transport system in the brush-border membrane. The effects of parathyrin on Na+ and glucose transport, however, seem to be due to alterations to the driving forces for transport and not to the brush-border transport systems.

scholarly journals Studies on the orientation of brush-border membrane vesicles

1978 ◽  
Vol 172 (1) ◽  
pp. 57-62 ◽  
Author(s):  
W Haase ◽  
A Schäfer ◽  
H Murer ◽  
R Kinne
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Kidney ◽  
Freeze Fracture ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Immunological Methods ◽  
Kidney Cortex ◽  
Asymmetric Distribution ◽  
Rat Kidney Cortex

Orientation of rat renal and intestinal brush-border membrane vesicles was studied with two independent methods: electron-microscopic freeze-fracture technique and immunological methods. With the freeze-fracture technique a distinct asymmetric distribution of particles on the two membrane fracture faces was demonstrated; this was used as a criterion for orientation of the isolated membrane vesicles. For the immunological approach the accessibility or inaccessibility of aminopeptidase M localized on the outer surface of the cell membrane to antibodies was used. With both methods we showed that the brush-border membrane vesicles isolated from rat kidney cortex and from rat small intestine for transport studies are predominantly orientated right-side out.

Adenosine (Ado) uptake in brush-border membrane vesicles from rat kidney (BBM)

1991 ◽  
Vol 19 (3) ◽  
pp. 323S-323S ◽  
Author(s):  
J. SAYOS ◽  
J. J. CENTELLES ◽  
J. MALLOL ◽  
E. I. CANELA ◽  
C. LLUIS ◽  
...  
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Kidney ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles
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