Western blotting and isoform analysis of cathepsin D from normal and malignant human breast cell lines

1995 ◽  
Vol 35 (2) ◽  
pp. 211-220 ◽  
Author(s):  
Lisa D. Laury-Kleintop ◽  
Elizabeth C. Coronel ◽  
Marianne K. Lange ◽  
Thomas Tachovsky ◽  
Santo Longo ◽  
...  
Keyword(s):  
Cell Lines ◽  
Western Blotting ◽  
Cathepsin D ◽  
Human Breast ◽  
Breast Cell Lines ◽  
Human Breast Cell

scholarly journals GnRH receptor activation competes at a low level with growth signaling in stably transfected human breast cell lines

BMC Cancer ◽  
2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Kevin Morgan ◽  
Colette Meyer ◽  
Nicola Miller ◽  
Andrew H Sims ◽  
Ilgin Cagnan ◽  
...  
Keyword(s):  
Cell Lines ◽  
Receptor Activation ◽  
Gnrh Receptor ◽  
Human Breast ◽  
Low Level ◽  
Breast Cell Lines ◽  
Human Breast Cell

Cabbage Juices and Indoles Modulate the Expression Profile of AhR, ERα, and Nrf2 in Human Breast Cell Lines

2015 ◽  
Vol 67 (8) ◽  
pp. 1344-1356 ◽  
Author(s):  
Hanna Szaefer ◽  
Violetta Krajka-Kuźniak ◽  
Barbara Licznerska ◽  
Agnieszka Bartoszek ◽  
Wanda Baer-Dubowska
Keyword(s):  
Cell Lines ◽  
Expression Profile ◽  
Human Breast ◽  
Breast Cell Lines ◽  
Human Breast Cell

scholarly journals Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Carmen Ortiz ◽  
Luisa Morales ◽  
Miguel Sastre ◽  
William E. Haskins ◽  
Jaime Matta
Keyword(s):  
Essential Oil ◽  
Cell Lines ◽  
Double Strand Breaks ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Strand Breaks ◽  
Breast Cell Lines ◽  
Proteomics Approach ◽  
Human Breast Cell ◽  
Mcf 7

Sandalwood essential oil (SEO) is extracted fromSantalumtrees. Althoughα-santalol, a main constituent of SEO, has been studied as a chemopreventive agent, the genotoxic activity of the whole oil in human breast cell lines is still unknown. The main objective of this study was to assess the cytotoxic and genotoxic effects of SEO in breast adenocarcinoma (MCF-7) and nontumorigenic breast epithelial (MCF-10A) cells. Proteins associated with SEO genotoxicity were identified using a proteomics approach. Commercially available, high-purity, GC/MS characterized SEO was used to perform the experiments. The main constituents reported in the oil were (Z)-α-santalol (25.34%), (Z)-nuciferol (18.34%), (E)-β-santalol (10.97%), and (E)-nuciferol (10.46%). Upon exposure to SEO (2–8 μg/mL) for 24 hours, cell proliferation was determined by the MTT assay. Alkaline and neutral comet assays were used to assess genotoxicity. SEO exposure induced single- and double-strand breaks selectively in the DNA of MCF-7 cells. Quantitative LC/MS-based proteomics allowed identification of candidate proteins involved in this response: Ku70 (p=1.37E-2), Ku80 (p=5.8E-3), EPHX1 (p=3.3E-3), and 14-3-3ζ(p=4.0E-4). These results provide the first evidence that SEO is genotoxic and capable of inducing DNA single- and double-strand breaks in MCF-7 cells.

Human breast cell lines exhibit functional α2-adrenoceptors

2005 ◽  
Vol 58 (1) ◽  
pp. 50-61 ◽  
Author(s):  
Stella Maris Vázquez ◽  
Alejandro Gustavo Mladovan ◽  
Cecilia Pérez ◽  
Ariana Bruzzone ◽  
Alberto Baldi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Breast ◽  
Α2 Adrenoceptors ◽  
Breast Cell Lines ◽  
Human Breast Cell

scholarly journals BORIS (CTCFL) Is Not Expressed in Most Human Breast Cell Lines and High Grade Breast Carcinomas

PLoS ONE ◽  
2010 ◽  
Vol 5 (3) ◽  
pp. e9738 ◽  
Author(s):  
William C. Hines ◽  
Alexey V. Bazarov ◽  
Rituparna Mukhopadhyay ◽  
Paul Yaswen
Keyword(s):  
Cell Lines ◽  
High Grade ◽  
Breast Carcinomas ◽  
Human Breast ◽  
Breast Cell Lines ◽  
Human Breast Cell

scholarly journals Role of Metabolism in the Effects of Genistein and Its Phase II Conjugates on the Growth of Human Breast Cell Lines

2012 ◽  
Vol 14 (2) ◽  
pp. 329-344 ◽  
Author(s):  
Bo Yuan ◽  
Linglan Wang ◽  
Yi Jin ◽  
Huijuan Zhen ◽  
Pingwei Xu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Phase Ii ◽  
Human Breast ◽  
Breast Cell Lines ◽  
Human Breast Cell
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