Nonisothermal membrane phenomena across perfluorosulfonic acid-type membranes, Flemion S: Part II. Thermal membrane potential and transported entropy of ions

1994 ◽  
Vol 272 (8) ◽  
pp. 979-985 ◽  
Author(s):  
K. Hanaoka ◽  
R. Kiyono ◽  
M. Tasaka
Keyword(s):  
Membrane Potential ◽  
Perfluorosulfonic Acid ◽  
Acid Type ◽  
Transported Entropy

Thermoosmosis and Transported Entropy of Water across Hydrocarbonsulfonic Acid-Type Cation-Exchange Membranes

1995 ◽  
Vol 68 (2) ◽  
pp. 493-501 ◽  
Author(s):  
Takashi Suzuki ◽  
Keiko Iwano ◽  
Ryotaro Kiyono ◽  
Masayasu Tasaka
Keyword(s):  
Cation Exchange ◽  
Acid Type ◽  
Transported Entropy

scholarly journals Thermal membrane potential across test anion-exchange membrane ACIPLEX STA and the transported entropy of counterions.

MEMBRANE ◽  
1993 ◽  
Vol 18 (6) ◽  
pp. 363-370 ◽  
Author(s):  
Kokichi HANAOKA ◽  
Ryotaro KIYONO ◽  
Masayasu TASAKA ◽  
Masato HAMADA ◽  
Kiyotaka YOSHIE
Keyword(s):  
Membrane Potential ◽  
Anion Exchange ◽  
Anion Exchange Membrane ◽  
Transported Entropy ◽  
Exchange Membrane

scholarly journals Structural Determinants for the Mode of Action of the Imidazopyridine DS2 at d-containing g-Aminobutyric Acid Type A (GABAA) Receptors

2020 ◽  
Author(s):  
Bente Frølund ◽  
Frederik Rostrup ◽  
Christina Birkedahl Falk-Petersen ◽  
Kasper Harpsøe ◽  
Stine Buchleithner ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Gabaa Receptors ◽  
Mode Of Action ◽  
Aminobutyric Acid ◽  
Functional Assay ◽  
Pharmacological Profile ◽  
Structural Determinants ◽  
Acid Type ◽  
Plate Reader ◽  
Sar Study

<div>A SAR study of the delta-selective positive modulators DS2 was performed to assist the quest for the binding site. The modulatory effect was measured using a fluorometric inaging plate reader (FLIPR) membrane potential (FMP) functional assay. Specific positions in the structural scaffold of DS2 was found to severly affect the pharmacological profile. <br></div><div>Analogs superior to DS2 were identified displaying higher potency and selectivity for the alfa4beta1delta over alfa4beta1gamma.<br></div><br>

scholarly journals Structural Determinants for the Mode of Action of the Imidazopyridine DS2 at d-containing g-Aminobutyric Acid Type A (GABAA) Receptors

2020 ◽  
Author(s):  
Bente Frølund ◽  
Frederik Rostrup ◽  
Christina Birkedahl Falk-Petersen ◽  
Kasper Harpsøe ◽  
Stine Buchleithner ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Gabaa Receptors ◽  
Mode Of Action ◽  
Aminobutyric Acid ◽  
Functional Assay ◽  
Pharmacological Profile ◽  
Structural Determinants ◽  
Acid Type ◽  
Plate Reader ◽  
Sar Study

<div>A SAR study of the delta-selective positive modulators DS2 was performed to assist the quest for the binding site. The modulatory effect was measured using a fluorometric inaging plate reader (FLIPR) membrane potential (FMP) functional assay. Specific positions in the structural scaffold of DS2 was found to severly affect the pharmacological profile. <br></div><div>Analogs superior to DS2 were identified displaying higher potency and selectivity for the alfa4beta1delta over alfa4beta1gamma.<br></div><br>

An Electron Microscopic Study of an Avian Reovirus that Causes Arthritis

1971 ◽  
Vol 29 ◽  
pp. 254-255
Author(s):  
E, R. Walker ◽  
N. O. Olson ◽  
M. H. Friedman
Keyword(s):  
Electron Microscopy ◽  
Viral Genome ◽  
Inner Core ◽  
Avian Reovirus ◽  
Electron Microscopic ◽  
Outer Coat ◽  
Acid Type ◽  
Chicken Eggs ◽  
Icosahedral Particle ◽  
Mature Virus

An unidentified virus, responsible for an arthritic-like condition in chickens was studied by electron microscopy and other methods of viral investigation. It was characterized in chorio-allantoic membrane (CAM) lesions of embryonating chicken eggs and in tissue culture as to: 1) particle size; 2) structure; 3) mode of replication in the cell; and 4) nucleic acid type.The inoculated virus, coated and uncoated, is first seen in lysosomal-like inclusions near the nucleus; the virions appear to be uncoated in these electron dense inclusions (Figure 1), Although transfer of the viral genome from these inclusions is not observable, replicating virus and mature virus crystals are seen in the cytoplasm subsequent to the uncoating of the virions.The crystals are formed in association with a mass of fibrils 50 to 80 angstroms in diameter and a ribosome-studded structure that appears to be granular endoplasmic reticulum adapted to virus replication (Figure 2). The mature virion (Figure 3) is an icosahedral particle approximately 75 millimicrons in diameter. The inner core is 45 millimicrons, the outer coat 15 millimicrons, and the virion has no envelope.

Measurement of spontaneous neuronal membrane activity by time lapse confocal microscopy

1995 ◽  
Vol 53 ◽  
pp. 972-973
Author(s):  
R H. Selinfreund ◽  
A. H. Cornell-Bell
Keyword(s):  
Membrane Potential ◽  
Confocal Microscopy ◽  
Patch Clamp ◽  
Electrical Activity ◽  
Time Lapse ◽  
Neuronal Firing ◽  
Neuronal Membrane ◽  
Pituitary Cells ◽  
Patch Clamp Recording ◽  
Spontaneous Electrical Activity

Cellular electrophysiological properties are normally monitored by standard patch clamp techniques . The combination of membrane potential dyes with time-lapse laser confocal microscopy provides a more direct, least destructive rapid method for monitoring changes in neuronal electrical activity. Using membrane potential dyes we found that spontaneous action potential firing can be detected using time-lapse confocal microscopy. Initially, patch clamp recording techniques were used to verify spontaneous electrical activity in GH4\C1 pituitary cells. It was found that serum depleted cells had reduced spontaneous electrical activity. Brief exposure to the serum derived growth factor, IGF-1, reconstituted electrical activity. We have examined the possibility of developing a rapid fluorescent assay to measure neuronal activity using membrane potential dyes. This neuronal regeneration assay has been adapted to run on a confocal microscope. Quantitative fluorescence is then used to measure a compounds ability to regenerate neuronal firing.The membrane potential dye di-8-ANEPPS was selected for these experiments. Di-8- ANEPPS is internalized slowly, has a high signal to noise ratio (40:1), has a linear fluorescent response to change in voltage.

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