Errors introduced by a tritium label in position 8 of catecholamines

1986 ◽  
Vol 332 (1) ◽  
pp. 34-42 ◽  
Author(s):  
M. Grohmann ◽  
M. Henseling ◽  
L. Cassis ◽  
U. Trendelenburg
Keyword(s):  
Tritium Label

scholarly journals Arachidonic acid incorporation by cytoplasmic lipid bodies of human eosinophils

Blood ◽  
1985 ◽  
Vol 65 (5) ◽  
pp. 1269-1274 ◽  
Author(s):  
PF Weller ◽  
AM Dvorak
Keyword(s):  
Arachidonic Acid ◽  
Neutral Lipids ◽  
Spherical Shape ◽  
Arachidonic Acid Metabolism ◽  
Lipid Bodies ◽  
Electron Microscopic ◽  
Electron Microscopic Autoradiography ◽  
Normal Peripheral Blood ◽  
Tritium Label ◽  
Almost All

Abstract The presence of cytoplasmic lipid bodies in human eosinophils and the participation of these lipid bodies in the metabolism of arachidonic acid by human eosinophils have been studied. Lipid bodies, structures of roughly spherical shape and variable size within the cytoplasm, were identified with transmission electron microscopy by their shape and variable osmiophilia and by their lack of a limiting membrane. While generally absent from eosinophils of normal peripheral blood, lipid bodies were found in tissue eosinophils and in blood eosinophils from patients with eosinophilia. A role for lipid bodies in arachidonic acid metabolism was determined with eosinophils obtained from two eosinophilic patients. After incubation for 30 to 60 minutes with 3H- arachidonic acid, purified eosinophils took up 50% to 98% of the tritium label. By electron microscopic autoradiography, almost all tritium label was localized to lipid bodies. Only 3.6% of the cell- incorporated tritium label was free arachidonic acid, while 5.8% was neutral lipids and 66% was phospholipid. Thus, most of the tritiated arachidonic acid incorporated by human eosinophils was present in esterified form, predominantly as phospholipids, and almost all of the tritiated lipids were localized ultrastructurally to cytoplasmic lipid bodies. These results provide evidence that lipid bodies of human eosinophils have a role in the cellular metabolism of arachidonic acid.

scholarly journals ON THE PERSISTENCE OF OOCYTE NUCLEI FROM FETUS TO MATURITY IN THE LABORATORY MOUSE

1962 ◽  
Vol 12 (1) ◽  
pp. 169-175 ◽  
Author(s):  
George T. Rudkin ◽  
Helen A. Griech
Keyword(s):  
Germ Cell ◽  
Adult Female ◽  
Tritiated Thymidine ◽  
Post Partum ◽  
Laboratory Mouse ◽  
Follicle Cells ◽  
Fetal Life ◽  
Female Mice ◽  
Tritium Label ◽  
Prevailing View

Tritiated thymidine injected intraperitoneally into female mice midway through the gestation period was demonstrated by autoradiographic methods to be incorporated into the nuclei of oocytes of female embryos, observed at the pachytene stage of meiosis 2 to 4 days after the injection. The tritium label was also demonstrated in the oocyte nuclei of the daughters of similarly treated females at maturity (6 weeks post partum). It was also found that some follicle cells, likewise labeled with H3-thymidine in mid-fetal life, persisted to maturity with few or no intervening mitoses. The observations are presented in support of the prevailing view that individual oocytes which arise from germ cell primordia in fetal stages become the egg cells of the adult female mammal.

Introduction of a tritium label into nucleoside analogs modified in their carbohydrate residues

1988 ◽  
Vol 24 (1) ◽  
pp. 79-83
Author(s):  
L. A. Yakovleva ◽  
G. P. Akulov ◽  
A. I. Nagorskii ◽  
N. A. Patokina ◽  
Yu. L. Kaminskii ◽  
...  
Keyword(s):  
Nucleoside Analogs ◽  
Tritium Label

Distribution of tritium label in Dl-[G-3H] phenylalanine

1975 ◽  
Vol 11 (3) ◽  
pp. 435-444 ◽  
Author(s):  
Merwyn C. Clifford ◽  
E. Anthony Evans ◽  
A. Edward Kilner ◽  
David C. Warrell
Keyword(s):  
Tritium Label

Untersuchungen des Kohlenhydratstoffwechsels mit Wasserstoff-Markierung V

1968 ◽  
Vol 23 (3) ◽  
pp. 326-329 ◽  
Author(s):  
Helmut Simon ◽  
Ramiro Medina
Keyword(s):  
Tritium Content ◽  
Methyl Group ◽  
Tritium Label ◽  
Dehydrogenase Reaction ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Good Agreement

Is glucose, fructose or mannose fermented anaerobically with yeast in H2O/HOT, the ethanol formed shows 30% tritium in the methyl group, 48-54% in the 1-R-position (HNAD) and 44-52% in the 1-S-position (HDec)· The tritium content per gram atom hydrogen of the water is assumed to be 100 per cent. As the fermentation proceeds the tritium label in the 1-R-position increases. From glucose-1-T 30% and from glucose-6-T 24% of the tritium do not appeare in the ethanol formed.. 20% of the tritium label from glucose-2-T are incorporated into the methyl group of ethanol. Using glucose-1-14C and glucose-6-14C the pentose cycle was determined to be 9,7 per cent. This is in good agreement with the figure obtained with glucose-3-T. Glucose-1-T-6-phosphate is discriminated in the glucose-6-phosphate dehydrogenase reaction against the nonlabeled material.

Messung der T-Fixierung in einigen Zwischenprodukten nach Hefe-Gärung unter anaeroben und aeroben Bedingungen in H2O/HOT

1968 ◽  
Vol 23 (1) ◽  
pp. 64-68 ◽  
Author(s):  
Klaus Schmidt ◽  
Gerhard Müllhofer ◽  
Helmut Simon
Keyword(s):  
Single Step ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Tritium Content ◽  
Methyl Group ◽  
Tritium Label ◽  
Mannose 6 Phosphate ◽  
Good Agreement

Baker’s yeast was incubated anaerobically and aerobically in H2O/HOT with uniformly labeled 14C-glucose. After partial fermentation the tritium distribution in some intermediates was determined. The equilibriums glucose-6-phosphate ⇄ frucose 6-phosphate ⇄ mannose-6-phosphate has not been reached. The tritium distribution in mannose-6-phosphate markedly differs from that in glucose- and fructose-6-phosphate. In fructose-1,6-diphosphate C-3, 4 and 5 have obtained the highest tritium label possible. The tritium content in the methyl group of ethanol is in good agreement with that obtained by adding up the tritium incorporation of every single step determined experimentally.

Behavior of humic substances in the liquid-liquid system directly measured using tritium label

Chemosphere ◽  
2020 ◽  
Vol 238 ◽  
pp. 124646 ◽  
Author(s):  
Maria G. Chernysheva ◽  
Gennadii A. Badun ◽  
Natalia A. Kulikova ◽  
Irina V. Perminova
Keyword(s):  
Humic Substances ◽  
Liquid System ◽  
Tritium Label
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