scholarly journals An agar plate method for the quantitative determination of the decrease of activity of antibiotics in the presence of human blood

1960 ◽  
Vol 47 (16) ◽  
pp. 380-380
Author(s):  
Jozef Balan ◽  
Vladimìr Betina
Keyword(s):  
Quantitative Determination ◽  
Human Blood ◽  
Agar Plate ◽  
Plate Method

scholarly journals Validation Parameters as Applied to Methods for Quantification of Microorganisms in Medicinal Products

2020 ◽  
Vol 10 (4) ◽  
pp. 267-272
Author(s):  
I. A. Buylova ◽  
O. V Gunar
Keyword(s):  
Quantitative Determination ◽  
Agar Plate ◽  
Test Method ◽  
Medicinal Products ◽  
Plate Method ◽  
Microbiological Methods ◽  
Study Results ◽  
Validation Parameters ◽  
Limit Of Quantitation

Validation/verification of microbiological methods is a prerequisite for quality control of non-sterile drugs. However, the use of existing procedures for validation/verification of analytical methods is challenging, since a number of factors, such as microorganism distribution in the sample, cell morphology, and metabolic activity of microorganisms contribute to the error in microbiological testing.The aim of the study was to assess the feasibility of using the microbiological method validation parameters for validation/verification of the agar plate method.Materials and methods: 18 non-sterile medicinal products were used in the study. Experiments included determination of antimicrobial activity. The quantification of viable bacteria, yeasts and moulds was performed using the modified pour plate method. The statistical processing of the obtained results was performed using Microsoft Excel 7.0 and Statistica 8.0.Results: the paper provides the results of quantitative determination of test microorganisms inoculated into non-sterile drugs. The results were obtained as part of validation/verification of the agar plate method of the State Pharmacopoeia of the Russian Federation, XIV ed.Conclusions: the validation/verification of the test method for isolation and quantification of microorganisms revealed no deviations of the study results from the established acceptance criteria. This proves the feasibility of using the following validation parameters: accuracy, precision, robustness, and limit of quantitation when validating new methods for quantitative determination of microorganisms or verification of previously validated methods.

scholarly journals Development and validation of method for quantitative determination of sildenafil and n-desmethyl sildenafil by HPLC-MS/MS in human blood plasma

2017 ◽  
Vol 0 (1 (5)) ◽  
pp. 22-32 ◽  
Author(s):  
Igor Kuznetsov ◽  
Helen Naumenko ◽  
Nataliia Reznichenko ◽  
Andrey Kostiuk ◽  
Roman Savyak ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Blood Plasma ◽  
Human Blood ◽  
Human Blood Plasma ◽  
Development And Validation

scholarly journals Quantitative Determination of Estradiol Fatty Acid Esters in Lipoprotein Fractions in Human Blood

2003 ◽  
Vol 88 (6) ◽  
pp. 2552-2555 ◽  
Author(s):  
Veera Vihma ◽  
Aila Tiitinen ◽  
Olavi Ylikorkala ◽  
Matti J. Tikkanen
Keyword(s):  
Fatty Acid ◽  
Quantitative Determination ◽  
Human Blood ◽  
Fatty Acid Esters ◽  
Acid Esters

scholarly journals Application of casein agar plate method for the determination of protease activity.

Eisei kagaku ◽  
1988 ◽  
Vol 34 (3) ◽  
pp. 241-247 ◽  
Author(s):  
KATSUSHI YOKOTA ◽  
NAOMI FURUSAWA ◽  
TETSUYA ABE ◽  
SEIKO TAKENAKA
Keyword(s):  
Protease Activity ◽  
Agar Plate ◽  
Plate Method

Development of an HPLC-MS/MS Method for Quantitative Determination of Rivaroxaban in Human Blood Serum

2018 ◽  
Vol 52 (4) ◽  
pp. 372-377 ◽  
Author(s):  
T. A. Rodina ◽  
E. S. Mel’nikov ◽  
A. A. Aksenov ◽  
S. A. Belkov ◽  
A. V. Sokolov ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Blood Serum ◽  
Human Blood ◽  
Human Blood Serum
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