Measurement of hepatic drug-metabolizing enzyme activity in man

1980 ◽  
Vol 17 (4) ◽  
pp. 267-274 ◽  
Author(s):  
E. A. Sotaniemi ◽  
R. O. Pelkonen ◽  
M. Puukka
Keyword(s):  
Enzyme Activity ◽  
Hepatic Drug ◽  
Drug Metabolizing Enzyme ◽  
Metabolizing Enzyme

scholarly journals Hepatic drug-metabolizing enzyme activity in rats pretreated with (−)-emetine or (±)-2,3-dehydroemetine

1970 ◽  
Vol 117 (3) ◽  
pp. 491-498 ◽  
Author(s):  
H. H. Miller ◽  
R. K. Johnson ◽  
J. D. Donahue ◽  
W. R. Jondorf
Keyword(s):  
Enzyme Activity ◽  
Female Rats ◽  
Inhibitory Effects ◽  
Hepatic Drug ◽  
Azo Reduction ◽  
Drug Metabolizing Enzyme ◽  
Metabolizing Enzyme ◽  
Sodium Phenobarbital ◽  
Stimulation Of

1. Pretreatment of female rats with (−)-emetine or (±)-2,3-dehydroemetine (at 18μmol/kg body wt. for 24h) prolongs the hexobarbital-induced sleeping-time of the treated animals. 2. This effect is not observed on pretreating animals with other compounds closely related to (−)-emetine, such as (−)-isoemetine or (+)-O-methylpsychotrine. 3. Liver microsomal drug-metabolizing enzyme activity in vitro as measured by N-demethylation of aminopyrine and azo-reduction of Neoprontosil is inhibited in rats pretreated with (−)-emetine or with (±)-2,3-dehydroemetine. 4. These inhibitory effects on drug metabolism in vitro are not observed in corresponding experiments involving pretreatment of rats with (−)-isoemetine or (+)-O-methylpsychotrine. 5. Co-administration of emetine or 2,3-dehydroemetine and sodium phenobarbital or 1,1-dichloro-2-o-chlorophenyl-2-p-chlorophenylethane to rats abolishes or greatly diminishes the stimulation of drug-metabolizing enzyme activity in vitro usually obtained by the administration of phenobarbital or 1,1-dichloro-2-o-chlorophenyl-2-p-chlorophenylethane alone. 6. Further, in rats pretreated with sodium phenobarbital and subsequently injected with emetine or 2,3-dehydroemetine the pre-stimulated drug-metabolizing enzyme activity in vitro is diminished. 7. The inhibitory effects on drug-metabolizing enzyme activity after pretreatment with (−)-emetine or (±)-2,3-dehydroemetine do not appear to be related to NADPH generation.

scholarly journals The role of nitric oxide on decreasing the hepatic drug metabolizing enzyme activity by bacterial endotoxin in rats

1999 ◽  
Vol 79 ◽  
pp. 125
Author(s):  
Kiyoyuki Kitaichi ◽  
Li Wang ◽  
Haruna Kidokoro ◽  
Mitsunori Iwase ◽  
Kenji Takagi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Enzyme Activity ◽  
Bacterial Endotoxin ◽  
Hepatic Drug ◽  
Drug Metabolizing Enzyme ◽  
Metabolizing Enzyme

Allergic Reaction to Antipyrine, A Marker of Hepatic Enzyme Activity

DICP ◽  
1989 ◽  
Vol 23 (1) ◽  
pp. 38-40 ◽  
Author(s):  
Jacqueline B. Mccrea ◽  
Peter H. Vlasses ◽  
Mario L. Rocci
Keyword(s):  
Clinical Trial ◽  
Enzyme Activity ◽  
Allergic Reaction ◽  
Pyrazole Derivative ◽  
Hepatic Enzyme ◽  
Upper Lip ◽  
Hepatic Drug ◽  
Drug Metabolizing Enzyme ◽  
Metabolizing Enzyme ◽  
Study Participants

Antipyrine is a pyrazole derivative used extensively as a marker for hepatic drug metabolizing enzyme activity. A subject participating in a clinical trial employing antipyrine experienced an acute allergic reaction to the drug which was characterized by diaphoresis, flushing, swelling of the throat, difficulty in breathing, vomiting, swelling of the upper lip, and a diffuse urticarial rash. Intravenous administration of diphenhydramine markedly improved the symptomatology. Clinical investigators as well as study participants should be alerted to the potential for antipyrine to cause an acute allergic reaction.

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