Pathological serum protein (cryoglobulin) as nucleic acid-protein complex

1964 ◽  
Vol 51 (24) ◽  
pp. 639-640 ◽  
Author(s):  
M. M. Fr�hlich ◽  
V. Bal�zs
Keyword(s):  
Nucleic Acid ◽  
Serum Protein ◽  
Protein Complex ◽  
Acid Protein

scholarly journals Atomic Force Microscopy Investigation of the Interactions between the MCM Helicase and DNA

Materials ◽  
2021 ◽  
Vol 14 (3) ◽  
pp. 687
Author(s):  
Amna Abdalla Mohammed Khalid ◽  
Pietro Parisse ◽  
Barbara Medagli ◽  
Silvia Onesti ◽  
Loredana Casalis
Keyword(s):  
Atomic Force Microscopy ◽  
Nucleic Acid ◽  
Single Molecule ◽  
Protein Complex ◽  
The Other ◽  
Contour Length ◽  
Force Microscopy ◽  
Atomic Force ◽  
Mcm Complex ◽  
Dna Double Helix

The MCM (minichromosome maintenance) protein complex forms an hexameric ring and has a key role in the replication machinery of Eukaryotes and Archaea, where it functions as the replicative helicase opening up the DNA double helix ahead of the polymerases. Here, we present a study of the interaction between DNA and the archaeal MCM complex from Methanothermobacter thermautotrophicus by means of atomic force microscopy (AFM) single molecule imaging. We first optimized the protocol (surface treatment and buffer conditions) to obtain AFM images of surface-equilibrated DNA molecules before and after the interaction with the protein complex. We discriminated between two modes of interaction, one in which the protein induces a sharp bend in the DNA, and one where there is no bending. We found that the presence of the MCM complex also affects the DNA contour length. A possible interpretation of the observed behavior is that in one case the hexameric ring encircles the dsDNA, while in the other the nucleic acid wraps on the outside of the ring, undergoing a change of direction. We confirmed this topographical assignment by testing two mutants, one affecting the N-terminal β-hairpins projecting towards the central channel, and thus preventing DNA loading, the other lacking an external subdomain and thus preventing wrapping. The statistical analysis of the distribution of the protein complexes between the two modes, together with the dissection of the changes of DNA contour length and binding angle upon interaction, for the wild type and the two mutants, is consistent with the hypothesis. We discuss the results in view of the various modes of nucleic acid interactions that have been proposed for both archaeal and eukaryotic MCM complexes.

scholarly journals AN INVESTIGATION OF THE FOLIC ACID-PROTEIN COMPLEX IN YEAST

1950 ◽  
Vol 182 (1) ◽  
pp. 367-384
Author(s):  
V.G. Allfrey ◽  
C.G. King
Keyword(s):  
Folic Acid ◽  
Protein Complex ◽  
Acid Protein

5S Ribonucleic acid-protein complex extracted from rat liver ribosomes by formamide

Biochemistry ◽  
1972 ◽  
Vol 11 (12) ◽  
pp. 2323-2326 ◽  
Author(s):  
Mary L. Petermann ◽  
Mary G. Hamilton ◽  
Amalia Pavlovec
Keyword(s):  
Rat Liver ◽  
Protein Complex ◽  
Ribonucleic Acid ◽  
Acid Protein ◽  
Liver Ribosomes

scholarly journals Differential Enzymatic 16O/18O Labeling for the Detection of Cross-Linked Nucleic Acid–Protein Heteroconjugates

2017 ◽  
Vol 89 (21) ◽  
pp. 11208-11213
Author(s):  
Fiona J. Flett ◽  
Timo Sachsenberg ◽  
Oliver Kohlbacher ◽  
C. Logan Mackay ◽  
Heidrun Interthal
Keyword(s):  
Nucleic Acid ◽  
Acid Protein ◽  
18O Labeling

Detection of nucleic acid–protein interactions in plant leaves using fluorescence lifetime imaging microscopy

2017 ◽  
Vol 12 (9) ◽  
pp. 1933-1950 ◽  
Author(s):  
Laurent Camborde ◽  
Alain Jauneau ◽  
Christian Brière ◽  
Laurent Deslandes ◽  
Bernard Dumas ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Lifetime ◽  
Protein Interactions ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Plant Leaves ◽  
Lifetime Imaging ◽  
Acid Protein

scholarly journals Ontogenetic changes in nucleic acid, protein contents, and growthof larval and juvenile Japanese flounder

2017 ◽  
Vol 41 ◽  
pp. 12-23 ◽  
Author(s):  
Xue Hong TONG ◽  
Cheng Man BAO ◽  
Xin Hui TANG ◽  
Xiao Lan YANG ◽  
Huan Li WANG
Keyword(s):  
Nucleic Acid ◽  
Japanese Flounder ◽  
Ontogenetic Changes ◽  
Acid Protein

scholarly journals Nucleic acid protein crystallography facilitated by selenium nucleic acids (SeNA)

2019 ◽  
Vol 75 (a1) ◽  
pp. a158-a158
Author(s):  
Zhen Huang ◽  
Andrey Kovalevsky ◽  
Qianwei Zhao ◽  
Lillian Hu
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Protein Crystallography ◽  
Acid Protein
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