Assessment of high-energy phosphorus compounds in the rat kidney by in situ31P nuclear magnetic resonance spectroscopy: effect of ischemia and furosemide

1993 ◽  
Vol 21 (3) ◽  
pp. 193-197 ◽  
Author(s):  
Masayuki Takeda ◽  
Yasushi Katayama ◽  
Toshiki Tsutsui ◽  
Hitoshi Takahashi ◽  
Kazuhide Saito ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Rat Kidney ◽  
High Energy ◽  
Phosphorus Compounds ◽  
Resonance Spectroscopy ◽  
Nuclear Magnetic

scholarly journals Acute Cerebral Ischaemia: Concurrent Changes in Cerebral Blood Flow, Energy Metabolites, pH, and Lactate Measured with Hydrogen Clearance and 31P and 1H Nuclear Magnetic Resonance Spectroscopy. III. Changes following Ischaemia

1988 ◽  
Vol 8 (6) ◽  
pp. 816-821 ◽  
Author(s):  
Kathryn Allen ◽  
Albert L. Busza ◽  
H. Alan Crockard ◽  
Richard S. J. Frackowiak ◽  
David G. Gadian ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
High Energy ◽  
Resonance Spectroscopy ◽  
High Energy Phosphates ◽  
Bilateral Carotid ◽  
Hydrogen Clearance ◽  
Nuclear Magnetic

CBF has been measured with the hydrogen clearance technique in the two cerebral hemispheres of the gerbil under halothane anaesthesia. At the same time, intracellular pH and the concentrations of lactate and high-energy phosphates were measured in the brain using 1H and 31P nuclear magnetic resonance spectroscopy. Flow and metabolism have been followed during either a 15- or a 30-min ischaemic period (induced by bilateral carotid occlusion) and for up to 1 h of recovery. There was no significant difference between the flow characteristics of the two experimental groups. High-energy phosphate levels and pH returned to control within ∼20 min of the end of the ischaemic period. Lactate clearance, following a 30-min occlusion, was slower than the recovery of pH. The concentration of free ADP, calculated from the creatine kinase equilibrium, was lower during the recovery phase than under control conditions.

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