A sensitive method for determination of renin activity in the single juxtaglomerular apparatus of the rat kidney

1970 ◽  
Vol 321 (4) ◽  
pp. 303-315 ◽  
Author(s):  
H. Dahlheim ◽  
P. Granger ◽  
K. Thurau
Keyword(s):  
Rat Kidney ◽  
Juxtaglomerular Apparatus ◽  
Renin Activity ◽  
Sensitive Method

Alcohol Determination by HPLC with Postcolumn Derivatization Based on the Thiosulfate-catalyzed Reaction of Alcohols and Cerium(IV) and Fluorescence Detection of Cerium(III)

2020 ◽  
Vol 16 ◽  
Author(s):  
Ikko Mikami ◽  
Eri Shibayama ◽  
Kengo Takagi
Keyword(s):  
Detection Limit ◽  
Fluorescence Detection ◽  
Reaction Rate ◽  
Detection Method ◽  
Fluorescence Detector ◽  
Reaction Solution ◽  
Optimum Detection ◽  
Postcolumn Derivatization ◽  
Sensitive Method

Background: Determination of a reducing substance based on the reaction between Ce(IV) and a reducing substance and fluorescence detection of Ce(III) generated has been reported as a selective and sensitive method. However, this method could not be applied to the determination of alcohol due to the low reaction rate of alcohol and Ce(IV). Objective: We found that thiosulfate catalytically enhanced reaction of alcohols (such as, methanol, ethanol, and propanol) and Ce(IV). Utilizing this effect, we developed a new method for the determination of alcohols. Results: In the presence of thiosulfate, an increase in fluorescence intensity was detected by injecting alcohol at concentrations of several millimolar, whereas it was not observed even at the concentration of 10% v/v (2 M for ethanol) in the absence of thiosulfate. The optimum detection conditions were determined to be 4.0 mM Ce(IV) sulfate and 0.50 mM thiosulfate, and the detection limit (S/N = 3) of ethanol under these conditions was 1 mM. In the calibration curves, changes in the slope were observed when the alcohol concentrations were approximately 10–25 mM. Using a thiosulfate solution containing ethanol as the reaction solution, a calibration curve without any change in slope was obtained, although the concentration of ethanol at the detection limit increased. The alcohols in the liquor and fuel were successfully analyzed using the proposed detection method as a postcolumn reaction. Conclusion: This new alcohol detection method using a versatile fluorescence detector can be applied to the postcolumn reaction of HPLC omitting need of time-consuming pretreatment processes.

A sensitive method for the determination of levamisole in serum by electrochemiluminescence

Luminescence ◽  
2013 ◽  
Vol 29 (2) ◽  
pp. 183-187 ◽  
Author(s):  
Ying Xiao ◽  
Jianping Li ◽  
Cong Fu
Keyword(s):  
Sensitive Method

scholarly journals Identification of vasopressin and determination of its corticomedullary levels in rat kidney tissue

1984 ◽  
Vol 26 (6) ◽  
pp. 785-790 ◽  
Author(s):  
Kurakazu Shimizu ◽  
Akihide Nakao ◽  
Tatsuya Nonaka ◽  
Hiroshi Oka
Keyword(s):  
Rat Kidney ◽  
Kidney Tissue
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