Technical problems in the micropuncture determination of nephron filtration rate and their functional implications

1971 ◽  
Vol 329 (4) ◽  
pp. 307-320 ◽  
Author(s):  
J. Schnermann ◽  
J. M. Davis ◽  
P. Wunderlich ◽  
D. Z. Levine ◽  
M. Horster
1972 ◽  
Vol 333 (3) ◽  
pp. 271-280 ◽  
Author(s):  
J. M. Davis ◽  
J. Schnermann ◽  
M. Horster

1994 ◽  
Vol 35 (4) ◽  
pp. 391-395
Author(s):  
S. Lundqvist ◽  
S. -O. Hietala ◽  
C. Berglund ◽  
K. Karp

GEGET ◽  
2002 ◽  
Vol 2 (1) ◽  
pp. 15-23
Author(s):  
Mervat Hesham ◽  
Zeinab Dosouky ◽  
Doaa Tawfeek ◽  
Somayya Abd-Alla

1978 ◽  
Vol 77 (1) ◽  
pp. 57-69 ◽  
Author(s):  
R. J. MORIARTY ◽  
A. G. LOGAN ◽  
J. C. RANKIN

The reliability of [3H]inulin as a reference substance for the measurement of glomerular filtration rate (GFR) in river lampreys was investigated. Simultaneously measured renal clearances of [3H]inulin and [14C]-polyethylene glycol (PEG) were not significantly different. Recoveries of [3H]inulin in the urine following its injection into the proximal tubule and urinary duct averaged 83.5±4.0% (n = 14) and 93.0±4.6% (n = 9) respectively. No evidence was obtained to suggest penetration of the tubular wall by [3H]inulin following its introduction into peritubular capillaries. Gel-filtration of lamprey plasma and urine produced no signs of any significant degradation of the inulin to fructose. [3H]inulin recoveries, though significantly below 100% (P < 0.001), were considered adequate to justify its use after allowing for possible sources of error. A mean single nephron filtration rate (SNGFR) value of 7.02±0.27 nl/min (n = 89) was obtained from the kidneys of anaesthetized freshwater lampreys. [51Cr]EDTA was found to be totally unsuitable for the measurement of GFR in this species.


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