Self-splicing of a mitochondrial group I intron from the cytochromeb gene of the ascomycetePodospora anserina

1992 ◽  
Vol 233 (1-2) ◽  
pp. 71-80 ◽  
Author(s):  
Udo Schmidt ◽  
Eckhard Budde ◽  
Ulf Stahl
Keyword(s):  
Group I Intron ◽  
Group I ◽  
Cytochromeb Gene ◽  
Self Splicing

scholarly journals A Unique Group I Intron in Coxiella burnetii Is a Natural Splice Mutant

2009 ◽  
Vol 191 (12) ◽  
pp. 4044-4046 ◽  
Author(s):  
Rahul Raghavan ◽  
Linda D. Hicks ◽  
Michael F. Minnick
Keyword(s):  
Coxiella Burnetii ◽  
Group I Intron ◽  
23S Rrna ◽  
Rrna Gene ◽  
Group I Introns ◽  
Group I ◽  
23S Rrna Gene ◽  
Self Splicing

ABSTRACT Cbu.L1917, a group I intron present in the 23S rRNA gene of Coxiella burnetii, possesses a unique 3′-terminal adenine in place of a conserved guanine. Here, we show that, unlike all other group I introns, Cbu.L1917 utilizes a different cofactor for each splicing step and has a decreased self-splicing rate in vitro.

Crystal structure of a self-splicing group I intron with both exons

Nature ◽  
2004 ◽  
Vol 430 (6995) ◽  
pp. 45-50 ◽  
Author(s):  
Peter L. Adams ◽  
Mary R. Stahley ◽  
Anne B. Kosek ◽  
Jimin Wang ◽  
Scott A. Strobel
Keyword(s):  
Crystal Structure ◽  
Group I Intron ◽  
Group I ◽  
Self Splicing

scholarly journals A Self-Splicing Group I Intron in DNA Polymerase Genes of T7-Like Bacteriophages

2004 ◽  
Vol 186 (23) ◽  
pp. 8153-8155 ◽  
Author(s):  
Richard P. Bonocora ◽  
David A. Shub
Keyword(s):  
Dna Polymerase ◽  
Group I Intron ◽  
Group I Introns ◽  
Homing Endonucleases ◽  
Structural Element ◽  
Group I ◽  
Close Relatives ◽  
Self Splicing

ABSTRACT Group I introns are inserted into genes of a wide variety of bacteriophages of gram-positive bacteria. However, among the phages of enteric and other gram-negative proteobacteria, introns have been encountered only in phage T4 and several of its close relatives. Here we report the insertion of a self-splicing group I intron in the coding sequence of the DNA polymerase genes of ΦI and W31, phages that are closely related to T7. The introns belong to subgroup IA2 and both contain an open reading frame, inserted into structural element P6a, encoding a protein belonging to the HNH family of homing endonucleases. The introns splice efficiently in vivo and self-splice in vitro under mild conditions of ionic strength and temperature. We conclude that there is no barrier for maintenance of group I introns in phages of proteobacteria.

Hoechst 33258 Selectively Inhibits Group I Intron Self-Splicing by Affecting RNA Folding

ChemBioChem ◽  
2004 ◽  
Vol 5 (12) ◽  
pp. 1647-1652 ◽  
Author(s):  
Matthew D. Disney ◽  
Jessica L. Childs ◽  
Douglas H. Turner
Keyword(s):  
Rna Folding ◽  
Group I Intron ◽  
Hoechst 33258 ◽  
Group I ◽  
Self Splicing
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