Ouabain decreases apparent potassium-conductance in proximal tubules of the amphibian kidney

1985 ◽  
Vol 404 (2) ◽  
pp. 131-137 ◽  
Author(s):  
G. Messner ◽  
W. Wang ◽  
M. Paulmichl ◽  
H. Oberleithner ◽  
F. Lang
Keyword(s):  
Potassium Conductance ◽  
Proximal Tubules ◽  
Amphibian Kidney

Swelling-activated Chloride and Potassium Conductance in Primary Cultures of Mouse Proximal Tubules. Implication of KCNE1 Protein

2003 ◽  
Vol 193 (3) ◽  
pp. 153-170 ◽  
Author(s):  
H. Barri�re ◽  
I. Rubera ◽  
R. Belfodil ◽  
M. Tauc ◽  
N. Tonnerieux ◽  
...  
Keyword(s):  
Primary Cultures ◽  
Potassium Conductance ◽  
Proximal Tubules

Reduction of Potassium in Kidney Proximal Tubules to Aid in Electron Probe X-Ray Microanalysis of Calcium

1985 ◽  
Vol 43 ◽  
pp. 474-475
Author(s):  
A. LeFurgey ◽  
P. Ingram ◽  
L.J. Mandel
Keyword(s):  
Smooth Muscle ◽  
Proximal Tubule ◽  
Electron Probe ◽  
Clinical Applications ◽  
Proximal Tubules ◽  
Rabbit Kidney ◽  
Target Cells ◽  
X Ray ◽  
Freeze Dried

For quantitative determination of subcellular Ca distribution by electron probe x-ray microanalysis, decreasing (and/or eliminating) the K content of the cell maximizes the ability to accurately separate the overlapping K Kß and Ca Kα peaks in the x-ray spectra. For example, rubidium has been effectively substituted for potassium in smooth muscle cells, thus giving an improvement in calcium measurements. Ouabain, a cardiac glycoside widely used in experimental and clinical applications, inhibits Na-K ATPase at the cell membrane and thus alters the cytoplasmic ion (Na,K) content of target cells. In epithelial cells primarily involved in active transport, such as the proximal tubule of the rabbit kidney, ouabain rapidly (t1/2= 2 mins) causes a decrease2 in intracellular K, but does not change intracellular total or free Ca for up to 30 mins. In the present study we have taken advantage of this effect of ouabain to determine the mitochondrial and cytoplasmic Ca content in freeze-dried cryosections of kidney proximal tubule by electron probe x-ray microanalysis.

Renal Protective Effects of Empagliflozin via Inhibition of the EMT Program Associated with Suppression of Aberrant Glycolysis in Proximal Tubules

2019 ◽  
Author(s):  
Jinpeng Li ◽  
Susumu Takagi ◽  
Kyoko Nitta ◽  
Munehiro Kitada ◽  
Swayam P. Srivastava ◽  
...  
Keyword(s):  
Proximal Tubules ◽  
Protective Effects
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