A quantitative analysis of the acetylcholine-activated potassium current in single cells from frog atrium

1987 ◽  
Vol 409 (4-5) ◽  
pp. 454-461 ◽  
Author(s):  
Mark A. Simmons ◽  
H. Criss Hartzell
Keyword(s):  
Quantitative Analysis ◽  
Potassium Current ◽  
Single Cells ◽  
Frog Atrium

Intracellular calcium activates a chloride current in canine ventricular myocytes

1994 ◽  
Vol 267 (5) ◽  
pp. H1984-H1995 ◽  
Author(s):  
A. C. Zygmunt
Keyword(s):  
Potassium Current ◽  
Ventricular Myocytes ◽  
Single Cells ◽  
Reversal Potential ◽  
Outward Current ◽  
Chloride Current ◽  
Disulfonic Acid ◽  
Transient Outward Current ◽  
Triggered Arrhythmias ◽  
Inward Currents

The contribution of chloride and potassium to the 4-aminopyridine (4-AP)-resistant transient outward current was investigated in dog cardiac myocytes. Whole cell currents were recorded at 37 degrees C in single cells dissociated from epicardial and midmyocardial regions of the canine ventricle. Sodium-calcium exchange current and voltage-dependent transient outward potassium current (IA) were blocked in sodium-free solutions containing 2 mM 4-AP; sodium channels were inactivated by the -50-mV holding potential. When patch pipettes contained 0.4–0.8 mM ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid, voltage-clamp steps over the range -20 to +50 mV activated an inward calcium current (ICa) and a Ca(2+)-activated chloride current [ICl(Ca)]. ICl(Ca) was blocked by 200 microM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, 1 mM 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS), or reduction of external chloride. Independent of the presence of potassium, the reversal potential of the SITS-sensitive current varied with extracellular chloride, as predicted for a chloride-selective conductance. The bell-shaped current-voltage relation of ICl(Ca) has a threshold of -20 mV and a peak at +40 mV. No evidence could be found for a Ca(2+)-activated potassium current or a Ca(2+)-activated nonspecific cation current under these conditions. ICl(Ca) contributed to oscillatory inward currents at diastolic potentials in cells superfused by isoproterenol and high Ca2+, suggesting a role for this current in triggered arrhythmias associated with delayed afterdepolarizations. In the normal heart, ICl(Ca) is likely to contribute to rate- and rhythm-dependent repolarization of the cardiac action potential.

scholarly journals Stochasticity in the enterococcal sex pheromone response revealed by quantitative analysis of transcription in single cells

PLoS Genetics ◽  
2017 ◽  
Vol 13 (7) ◽  
pp. e1006878 ◽  
Author(s):  
Rebecca J. Breuer ◽  
Arpan Bandyopadhyay ◽  
Sofie A. O’Brien ◽  
Aaron M. T. Barnes ◽  
Ryan C. Hunter ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Sex Pheromone ◽  
Single Cells ◽  
Pheromone Response

scholarly journals Quantitative Analysis of Pathways Controlling Extrinsic Apoptosis in Single Cells

2008 ◽  
Vol 30 (1) ◽  
pp. 11-25 ◽  
Author(s):  
John G. Albeck ◽  
John M. Burke ◽  
Bree B. Aldridge ◽  
Mingsheng Zhang ◽  
Douglas A. Lauffenburger ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Single Cells ◽  
Extrinsic Apoptosis
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