GTP-binding proteins mediate acetylcholine inhibition of voltage dependent calcium channels in hippocampal neurons

1989 ◽  
Vol 413 (3) ◽  
pp. 319-321 ◽  
Author(s):  
Mauro Toselli ◽  
Hans Dieter Lux
Keyword(s):  
Calcium Channels ◽  
Hippocampal Neurons ◽  
Binding Proteins ◽  
Gtp Binding Proteins ◽  
Gtp Binding ◽  
Voltage Dependent ◽  
Voltage Dependent Calcium Channels

scholarly journals The RGK family of GTP-binding proteins: Regulators of voltage-dependent calcium channels and cytoskeleton remodeling

2008 ◽  
Vol 20 (2) ◽  
pp. 292-300 ◽  
Author(s):  
Robert N. Correll ◽  
Chunyan Pang ◽  
Dana M. Niedowicz ◽  
Brian S. Finlin ◽  
Douglas A. Andres
Keyword(s):  
Calcium Channels ◽  
Binding Proteins ◽  
Gtp Binding Proteins ◽  
Gtp Binding ◽  
Voltage Dependent ◽  
Voltage Dependent Calcium Channels ◽  
Cytoskeleton Remodeling

scholarly journals GTP-binding proteins mediate transmitter inhibition of voltage-dependent calcium channels

Nature ◽  
1986 ◽  
Vol 319 (6055) ◽  
pp. 670-672 ◽  
Author(s):  
George G. Holz ◽  
Stanley G. Rane ◽  
Kathleen Dunlap
Keyword(s):  
Calcium Channels ◽  
Binding Proteins ◽  
Gtp Binding Proteins ◽  
Gtp Binding ◽  
Voltage Dependent ◽  
Voltage Dependent Calcium Channels

Small GTP-binding Protein RalA Associates with Weibel-Palade Bodies in Endothelial Cells

1999 ◽  
Vol 82 (09) ◽  
pp. 1177-1181 ◽  
Author(s):  
Hubert de Leeuw ◽  
Pauline Wijers-Koster ◽  
Jan van Mourik ◽  
Jan Voorberg
Keyword(s):  
Endothelial Cells ◽  
Binding Proteins ◽  
Binding Protein ◽  
Control Release ◽  
Small Gtpase ◽  
Gtp Binding Protein ◽  
Two Dimensional Gel Electrophoresis ◽  
Gtp Binding Proteins ◽  
Dense Granules ◽  
Gtp Binding

SummaryIn endothelial cells von Willebrand factor (vWF) and P-selectin are stored in dense granules, so-called Weibel-Palade bodies. Upon stimulation of endothelial cells with a variety of agents including thrombin, these organelles fuse with the plasma membrane and release their content. Small GTP-binding proteins have been shown to control release from intracellular storage pools in a number of cells. In this study we have investigated whether small GTP-binding proteins are associated with Weibel-Palade bodies. We isolated Weibel-Palade bodies by centrifugation on two consecutive density gradients of Percoll. The dense fraction in which these subcellular organelles were highly enriched, was analysed by SDS-PAGE followed by GTP overlay. A distinct band with an apparent molecular weight of 28,000 was observed. Two-dimensional gel electrophoresis followed by GTP overlay revealed the presence of a single small GTP-binding protein with an isoelectric point of 7.1. A monoclonal antibody directed against RalA showed reactivity with the small GTP-binding protein present in subcellular fractions that contain Weibel-Palade bodies. The small GTPase RalA was previously identified on dense granules of platelets and on synaptic vesicles in nerve terminals. Our observations suggest that RalA serves a role in regulated exocytosis of Weibel-Palade bodies in endothelial cells.

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