Whole-cell and unitary Ca channel currents in mammalian intestinal smooth muscle cells: evidence for the existence of two types of Ca channels

1988 ◽  
Vol 411 (2) ◽  
pp. 229-231 ◽  
Author(s):  
M. Yoshino ◽  
T. Someya ◽  
A. Nishio ◽  
H. Yabu
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Intestinal Smooth Muscle ◽  
Ca Channel ◽  
Ca Channels ◽  
Whole Cell ◽  
Channel Currents

Multiple types of voltage-dependent Ca channels in mammalian intestinal smooth muscle cells

1989 ◽  
Vol 414 (4) ◽  
pp. 401-409 ◽  
Author(s):  
M. Yoshino ◽  
T. Someya ◽  
A. Nishio ◽  
K. Yazawa ◽  
T. Usuki ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Intestinal Smooth Muscle ◽  
Ca Channels ◽  
Voltage Dependent

Two Types of Ca Channels in Mammalian Intestinal Smooth Muscle Cells

1989 ◽  
Vol 560 (1 Calcium Chann) ◽  
pp. 124-126
Author(s):  
H. YABU ◽  
M. YOSHINO ◽  
T. SOMEYA ◽  
M. TOTSUKA
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Intestinal Smooth Muscle ◽  
Ca Channels

IGFBP-3 activates TGF-β receptors and directly inhibits growth in human intestinal smooth muscle cells

2004 ◽  
Vol 287 (4) ◽  
pp. G795-G802 ◽  
Author(s):  
John F. Kuemmerle ◽  
Karnam S. Murthy ◽  
Jennifer G. Bowers
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Nuclear Translocation ◽  
Thymidine Incorporation ◽  
Muscle Cells ◽  
Serine Phosphorylation ◽  
Intestinal Smooth Muscle ◽  
Igf I ◽  
Tgf Β1 ◽  
Igfbp 3

We have shown that human intestinal smooth muscle cells produce IGF-I and IGF binding protein-3 (IGFBP-3). Endogenous IGF-I acts in autocrine fashion to stimulate growth of these cells. IGFBP-3 inhibits the binding of IGF-I to its receptor and thereby inhibits IGF-I-stimulated growth. In several carcinoma cell lines and some normal cells, IGFBP-3 regulates growth independently of IGF-I. Two mechanisms for this effect have been identified: IGFBP-3 can directly activate transforming growth factor-β (TGF-β) receptors or it can undergo direct nuclear translocation. The aim of the present study was to determine whether IGFBP-3 acts independently of IGF-I and to characterize the mechanisms mediating this effect in human intestinal smooth muscle cells. The direct effects of IGFBP-3 were determined in the presence of an IGF-I receptor antagonist to eliminate its IGF-I-dependent effects. Affinity labeling of TGF-β receptors (TGF-βRI, TGF-βRII, and TGF-βRV) with 125I-labeled TGF-β1 showed that IGFBP-3 displaced binding to TGF-βRII and TGF-βRV in a concentration-dependent fashion. IGFBP-3 stimulated TGF-βRII-dependent serine phosphorylation (activation) of both TGF-βRI and of its primary substrate, Smad2(Ser465/467). IGFBP-3 also caused IGF-I-independent inhibition of basal [3H]thymidine incorporation. The effects of IGFBP-3 on Smad2 phosphorylation and on smooth muscle cell proliferation were independent of TGF-β1 and were abolished by transfection of Smad2 siRNA. Immunoneutralization of IGFBP-3 increased basal [3H]thymidine incorporation, implying that endogenous IGFBP-3 inhibits proliferation. We conclude that endogenous IGFBP-3 directly inhibits proliferation of human intestinal smooth muscle cells by activation of TGF-βRI and Smad2, an effect that is independent of its effect on IGF-I-stimulated growth.

Galanin contracts and relaxes guinea pig and canine intestinal smooth muscle cells through distinct receptors

1995 ◽  
Vol 108 (1) ◽  
pp. 3-11 ◽  
Author(s):  
Alain Botella ◽  
Michel Delvaux ◽  
Jean Fioramonti ◽  
Jacques Frexinos ◽  
Lionel Bueno
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Intestinal Smooth Muscle
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