Induction of normal and dystrophic mineralization by glycerophosphates in long-term bone organ culture

1992 ◽  
Vol 50 (6) ◽  
pp. 553-563 ◽  
Author(s):  
Helmtrud I. Roach
Keyword(s):  
Organ Culture ◽  
Bone Organ Culture

Bench-Top Validation Tests for Tissue-Engineered Arteries

2000 ◽  
Author(s):  
Shawn Chin Quee ◽  
Hai-Chao Han ◽  
David N. Ku
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Comprehensive Evaluation ◽  
Ex Vivo ◽  
Vascular Grafts ◽  
Living System ◽  
Organ Culture System ◽  
Flow Pressure ◽  
Validation Tests

Abstract Standard tests are needed for evaluating and comparing the mechanical and biological functions of tissue engineered arteries and other vascular grafts. We propose an ex vivo organ culture system as a living system for testing tissue-engineered vascular grafts. This bench-top organ culture system mimics the physiological environment of arteries including the flow, pressure, and the axial stretch. Arterial mechanical properties and physiologic functions including compliance, burst pressure, and contractile functions can be assessed before an expensive long-term animal test is initiated. Test results of natural arteries indicate that organ culture is a valid model for comprehensive evaluation of tissue-engineered vascular grafts.

Studies of lead transport in bone organ culture

1977 ◽  
Vol 26 (7) ◽  
pp. 650-652 ◽  
Author(s):  
John F. Rosen ◽  
Emma E. Wexler
Keyword(s):  
Organ Culture ◽  
Bone Organ Culture

Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture

2012 ◽  
Vol 116 (6) ◽  
pp. 729-735 ◽  
Author(s):  
Laura Fernández Bidondo ◽  
Mariana Pergola ◽  
Vanesa Silvani ◽  
Roxana Colombo ◽  
Josefina Bompadre ◽  
...  
Keyword(s):  
Organ Culture ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Monoxenic Culture ◽  
Root Organ Culture

LONG TERM ORGAN CULTURE OF HUMAN PROSTATE TISSUE IN A NASA-DESIGNED ROTATING WALL BIOREACTOR

1999 ◽  
Vol 161 (1) ◽  
pp. 290-297 ◽  
Author(s):  
LEONID MARGOLIS ◽  
STEVEN HATFILL ◽  
RODRIGO CHUAQUI ◽  
CATHY VOCKE ◽  
MICHAEL EMMERT-BUCK ◽  
...  
Keyword(s):  
Organ Culture ◽  
Prostate Tissue ◽  
Human Prostate ◽  
Rotating Wall ◽  
Human Prostate Tissue

Ametantrone inhibits prostaglandin — mediated resorption in bone organ culture

1984 ◽  
Vol 28 (4) ◽  
pp. 469-476 ◽  
Author(s):  
Margaret R. Warner ◽  
Mark S. Rappaport ◽  
Nancy S. Krieger ◽  
Raymond F. Novak ◽  
Paula H. Stern
Keyword(s):  
Organ Culture ◽  
Bone Organ Culture

scholarly journals Organomatics and organometrics: Novel platforms for long-term whole-organ culture

TECHNOLOGY ◽  
2014 ◽  
Vol 02 (01) ◽  
pp. 13-22 ◽  
Author(s):  
Bote G. Bruinsma ◽  
Martin L. Yarmush ◽  
Korkut Uygun
Keyword(s):  
Organ Culture ◽  
Ex Vivo ◽  
Regulatory Control ◽  
Computational Algorithms ◽  
Machine Perfusion ◽  
Organ Function ◽  
Organ Models ◽  
Term Maintenance ◽  
Organ Replacement

Organ culture systems are instrumental as experimental whole-organ models of physiology and disease, as well as preservation modalities facilitating organ replacement therapies such as transplantation. Nevertheless, a coordinated system of machine perfusion components and integrated regulatory control has yet to be fully developed to achieve long-term maintenance of organ function ex vivo. Here we outline current strategies for organ culture, or organomatics, and how these systems can be regulated by means of computational algorithms, or organometrics, to achieve the organ culture platforms anticipated in modern-day biomedicine.

HISTOLOGICAL AND ULTRASTRUCTURAL CHANGES IN THE PARS DISTALIS OF THE RABBIT PITUITARY IN ORGAN CULTURE

1974 ◽  
Vol 60 (1) ◽  
pp. 155-NP ◽  
Author(s):  
S. PATHAK ◽  
A. FISK
Keyword(s):  
Organ Culture ◽  
Granular Cell ◽  
Common Type ◽  
Ultrastructural Changes ◽  
Pars Distalis ◽  
Prolactin Cells ◽  
Dna And Rna ◽  
Intranuclear Rodlets ◽  
Maximum Proportion

SUMMARY Typical histological and ultrastructural changes that occur in the pars distalis of the rabbit pituitary after different periods of organ culture are described. The best technique for the maintenance of the maximum proportion of the explant was assessed by comparing cultures grown under different conditions. Explants in air with a medium buffered with N-2-hydroxyethylpiperazine-N1-2-ethanesulphonic acid (HEPES), not previously used in organ culture, proved more satisfactory than explants in carbogen with bicarbonate-buffered 199, and cultures were maintained for more than 3 weeks. The survival of cells was assessed on the basis of their cytological integrity; DNA- and RNA-fluorescence with acridine orange was a valuable indicator. Prolactin cells, which were few in uncultured controls, became the most common type of granular cell in long-term cultures. Cell modifications during culture included the development of a peripheral epithelioid layer and the appearance of numerous microvilli. Microfibrils, coated or smooth vesicles, lytic bodies, desmosomes and intranuclear rods became more common and intranuclear rodlets (fibrous or membranous structures) were identified. Cells often became more electron dense during long-term culture. Though there was an increase in the number of agranular cells during culture, identifiable granules were retained by many cells throughout culture.

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