scholarly journals Behaviour in aggregates of irradiated imaginal disk cells ofDrosophila

1973 ◽  
Vol 172 (3) ◽  
pp. 187-195 ◽  
Author(s):  
G. Morata ◽  
A. Garcia-Bellido
Keyword(s):  
Imaginal Disk

Preferential binding of imaginal disk cells to embryonic segments of Drosophila

Nature ◽  
1985 ◽  
Vol 313 (6001) ◽  
pp. 395-397 ◽  
Author(s):  
Ann Gauger ◽  
Richard G. Fehon ◽  
Gerold Schubiger
Keyword(s):  
Imaginal Disk ◽  
Preferential Binding

scholarly journals Cost-precision trade-off relation determines the optimal morphogen gradient for accurate biological pattern formation

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Yonghyun Song ◽  
Changbong Hyeon
Keyword(s):  
Target Gene ◽  
Characteristic Length ◽  
Morphogen Gradient ◽  
Local Source ◽  
Minimal Cost ◽  
Imaginal Disk ◽  
Animal Development ◽  
Boundary Position ◽  
Biological Pattern Formation ◽  
Boundary Location

Spatial boundaries formed during animal development originate from the pre-patterning of tissues by signaling molecules, called morphogens. The accuracy of boundary location is limited by the fluctuations of morphogen concentration that thresholds the expression level of target gene. Producing more morphogen molecules, which gives rise to smaller relative fluctuations, would better serve to shape more precise target boundaries; however, it incurs more thermodynamic cost. In the classical diffusion-depletion model of morphogen profile formation, the morphogen molecules synthesized from a local source display an exponentially decaying concentration profile with a characteristic length λ. Our theory suggests that in order to attain a precise profile with the minimal cost, λ should be roughly half the distance to the target boundary position from the source. Remarkably, we find that the profiles of morphogens that pattern the Drosophila embryo and wing imaginal disk are formed with nearly optimal λ. Our finding underscores the thermodynamic cost as a key physical constraint in the morphogen profile formation in Drosophila development.

An extensive 3′ cis-regulatory region directs the imaginal disk expression of decapentaplegic, a member of the TGF-beta family in Drosophila

Development ◽  
1991 ◽  
Vol 111 (3) ◽  
pp. 657-666 ◽  
Author(s):  
R.K. Blackman ◽  
M. Sanicola ◽  
L.A. Raftery ◽  
T. Gillevet ◽  
W.M. Gelbart
Keyword(s):  
Germ Line ◽  
Regulatory Region ◽  
Imaginal Disk ◽  
Tgf Beta ◽  
Signalling Molecule ◽  
Rna In Situ Hybridization ◽  
Imaginal Disks ◽  
Beta Galactosidase

The decapentaplegic (dpp) gene in Drosophila melanogaster encodes a TGF-beta-like signalling molecule that is expressed in a complex and changing pattern during development. One of dpp's contributions is to proximal-distal outgrowth of the adult appendages, structures derived from the larval imaginal disks. Appendage specific mutations of dpp fall in a 20 kb interval 3′ to the known dpp transcripts. Here, we directly test the hypothesis that these mutations define an extended 3′ cis-regulatory region. By analysis of germ-line transformants expressing a reporter gene, we show that sequences from this portion of the gene, termed the dppdisk region, are capable of directing expression comparable to that defined by RNA in situ hybridization. We localize two intervals of the dppdisk region that appear to account for much of the dpp spatial pattern in imaginal disks and discuss the positions of these important elements in terms of the genetics of dpp. Finally, we provide evidence to suggest that one of our constructs expresses beta-galactosidase in the early imaginal disk primordia in the embryo, at approximately the time when they are set aside from surrounding larval epidermal tissues. Thus, dpp may be involved directly in the determination of the imaginal disks.

Increased levels of the Drosophila Abelson tyrosine kinase in nerves and muscles: subcellular localization and mutant phenotypes imply a role in cell-cell interactions

Development ◽  
1992 ◽  
Vol 116 (4) ◽  
pp. 953-966 ◽  
Author(s):  
R.L. Bennett ◽  
F.M. Hoffmann
Keyword(s):  
Nervous System ◽  
Tyrosine Kinase ◽  
Expression Patterns ◽  
Apical Cell ◽  
Cell Interactions ◽  
Cell Junctions ◽  
Imaginal Disk ◽  
Abelson Tyrosine Kinase ◽  
Mutant Phenotypes ◽  
Cell Cell

Mutations in the Drosophila Abelson tyrosine kinase have pleiotropic effects late in development that lead to pupal lethality or adults with a reduced life span, reduced fecundity and rough eyes. We have examined the expression of the abl protein throughout embryonic and pupal development and analyzed mutant phenotypes in some of the tissues expressing abl. abl protein, present in all cells of the early embryo as the product of maternally contributed mRNA, transiently localizes to the region below the plasma membrane cleavage furrows as cellularization initiates. The function of this expression is not yet known. Zygotic expression of abl is first detected in the post-mitotic cells of the developing muscles and nervous system midway through embryogenesis. In later larval and pupal stages, abl protein levels are also highest in differentiating muscle and neural tissue including the photoreceptor cells of the eye. abl protein is localized subcellularly to the axons of the central nervous system, the embryonic somatic muscle attachment sites and the apical cell junctions of the imaginal disk epithelium. Evidence for abl function was obtained by analysis of mutant phenotypes in the embryonic somatic muscles and the eye imaginal disk. The expression patterns and mutant phenotypes indicate a role for abl in establishing and maintaining cell-cell interactions.

Role of the porcupine gene in the development of the wing imaginal disk of Drosophila melanogaster

2008 ◽  
Vol 44 (11) ◽  
pp. 1290-1295 ◽  
Author(s):  
S. A. Kopyl ◽  
N. V. Dorogova ◽  
T. Yu. Baimak ◽  
L. -S. Chang ◽  
L. V. Omelyanchuk
Keyword(s):  
Drosophila Melanogaster ◽  
Imaginal Disk ◽  
Wing Imaginal Disk

The relationship of decapentaplegic and engrailed expression in Drosophila imaginal disks: do these genes mark the anterior-posterior compartment boundary?

Development ◽  
1991 ◽  
Vol 113 (1) ◽  
pp. 27-33 ◽  
Author(s):  
L.A. Raftery ◽  
M. Sanicola ◽  
R.K. Blackman ◽  
W.M. Gelbart
Keyword(s):  
Reporter Gene ◽  
Ectopic Expression ◽  
Reporter Genes ◽  
Imaginal Disk ◽  
Compartment Boundary ◽  
Imaginal Disks ◽  
Relationship Of ◽  
The Relationship ◽  
Gene Expressing

Imaginal disks, the primordia of the adult appendages in Drosophila, are divided into anterior and posterior compartments. However, the developmental role of such compartments remains unclear. The expression of decapentaplegic (dpp), a pattern formation gene required for imaginal disk development, has the intriguing property of being expressed in a line at or near the boundary between these compartments. Here, we compare the distribution of dpp-driven reporter gene expression to the pattern of expression of the engrailed (en) gene, known to be required for the maintenance of the compartment boundary. Using confocal microscopy to obtain single cell resolution, we have determined that the majority of the en+ imaginal disk cells expressing the dpp-driven reporter genes about those cells expressing en, while a small percentage of dpp reporter gene expressing cells also express en. In posterior regions of en mutant disks, where compartmentalization is abnormal, we observe ectopic expression of the dpp-driven reporter genes. We conclude that the pattern of dpp expression in imaginal disks is delimited in part through the direct or indirect repression by engrailed. Our results lead us to question the widely held assumption that the anterior edge of en expression demarcates the A/P compartment boundary.

Determination, Activation and Actinomycin D Insensitivity in the Optic Imaginal Disk of Drosophila

Nature ◽  
1969 ◽  
Vol 221 (5184) ◽  
pp. 933-935 ◽  
Author(s):  
C. H. WADDINGTON ◽  
EVELYN ROBERTSON
Keyword(s):  
Actinomycin D ◽  
Imaginal Disk

scholarly journals dTcf/Pangolinsuppresses growth and tumor formation inDrosophila

2019 ◽  
Vol 116 (28) ◽  
pp. 14055-14064 ◽  
Author(s):  
Shilin Song ◽  
Diana Andrejeva ◽  
Flávia C. P. Freitas ◽  
Stephen M. Cohen ◽  
Héctor Herranz
Keyword(s):  
Transcription Factor ◽  
Target Genes ◽  
Normal Development ◽  
Tumor Development ◽  
Growth Control ◽  
Tissue Growth ◽  
Tumor Formation ◽  
Imaginal Disk ◽  
Animal Development ◽  
Transcription Factor 1

Wnt/Wingless (Wg) signaling controls many aspects of animal development and is deregulated in different human cancers. The transcription factor dTcf/Pangolin (Pan) is the final effector of the Wg pathway inDrosophilaand has a dual role in regulating the expression of Wg target genes. In the presence of Wg, dTcf/Pan interacts with β-catenin/Armadillo (Arm) and induces the transcription of Wg targets. In absence of Wg, dTcf/Pan partners with the transcriptional corepressor TLE/Groucho (Gro) and inhibits gene expression. Here, we use the wing imaginal disk ofDrosophilaas a model to examine the functions that dTcf/Pan plays in a proliferating epithelium. We report a function of dTcf/Pan in growth control and tumorigenesis. Our results show that dTcf/Pan can limit tissue growth in normal development and suppresses tumorigenesis in the context of oncogene up-regulation. We identify the conserved transcription factorsSox box protein 15(Sox15) andFtz transcription factor 1(Ftz-f1) as genes controlled by dTcf/Pan involved in tumor development. In conclusion, this study reports a role for dTcf/Pan as a repressor of normal and oncogenic growth and identifies the genes inducing tumorigenesis downstream of dTcf/Pan.

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