Contractile response and electrophysiological properties in enzymatically dispersed smooth muscle cells of rat vas deferens

1987 ◽  
Vol 408 (2) ◽  
pp. 112-119 ◽  
Author(s):  
Ken Nakazawa ◽  
Norio Matsuki ◽  
Koki Shigenobu ◽  
Yutaka Kasuya
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vas Deferens ◽  
Contractile Response ◽  
Muscle Cells ◽  
Rat Vas Deferens ◽  
Electrophysiological Properties

Neurokinin A suppresses a voltage-gated K+ current in smooth muscle cells from rat vas deferens

1990 ◽  
Vol 182 (1) ◽  
pp. 189-192 ◽  
Author(s):  
Ken Nakazawa ◽  
Kazuhide Inoue ◽  
Kannosuke Fujimori ◽  
Akira Takanaka
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vas Deferens ◽  
Muscle Cells ◽  
Rat Vas Deferens ◽  
Neurokinin A ◽  
Voltage Gated ◽  
K Current

scholarly journals Electrophysiological recordings from spontaneously contracting reaggregates of cultured smooth muscle cells from guinea pig vas deferens.

1979 ◽  
Vol 80 (3) ◽  
pp. 539-552 ◽  
Author(s):  
M J McLean ◽  
A Pelleg ◽  
N Sperelakis
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Action Potentials ◽  
Vas Deferens ◽  
Input Resistance ◽  
Muscle Cells ◽  
Mean Value ◽  
Electrophysiological Properties ◽  
Culture Cells

Smooth muscle cells were enzymatically dispersed from vasa deferentia of adult male guinea pigs (250-400 g). These cells reassociated in vitro to form monolayers and small spherical reaggregates (0.05-0.3 mm in Diam). Within 48 h of being placed in culture, cells in both types of preparation began to contract spontaneously. The contractions were rhythmic and slow. Cells in the monolayers stopped contracting after approximately 1 wk in vitro, but the reaggregates continued to contract spontaneously for at least 3 wk. Electron microscopy of the reaggregates revealed the presence of thick and thin myofilaments. Overshooting action potentials were recorded in many of the cells penetrated (primarily in reaggregates), and were accompanied by visible contractions of the aggregate or monolayer. Quiescent cells could often be excited by intracellularly applied depolarizing and hyperpolarizing (anodal-break) current pulses. The resting potentials had a mean value of -58 +/- 2 mV. The action potentials were usually preceded by a spontaneous depolarization. The action potentials had slow rates of rise (1--4 V/s) which were unaffected by tetrodotoxin (TTX, 1 microgram/ml), a known blocker of fast Na+ -channels. Verapamil (1 microgram/ml) blocked the action potentials. The mean value of input resistance was 6.9 +/- 0.5 M omega (n = 12). These electrophysiological properties are similar to those of intact adult vas deferens smooth muscle cells. Thus, the cultured adult vas deferens smooth muscle cells retain their functional properties in vitro even after long periods.

scholarly journals TTX-sensitive Na+ and nifedipine-sensitive Ca2+ channels in rat vas deferens smooth muscle cells

1999 ◽  
Vol 1419 (2) ◽  
pp. 343-352 ◽  
Author(s):  
Andriy E. Belevych ◽  
Aleksiy V. Zima ◽  
Irina A. Vladimirova ◽  
Hanako Hirata ◽  
Aron Jurkiewicz ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vas Deferens ◽  
Muscle Cells ◽  
Rat Vas Deferens ◽  
Ca2 Channels
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