Identification and characterization of major ionic currents in isolated smooth muscle cells using the voltage-clamp technique

1987 ◽  
Vol 408 (2) ◽  
pp. 83-97 ◽  
Author(s):  
John V. Walsh ◽  
Joshua J. Singer
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Voltage Clamp ◽  
Muscle Cells ◽  
Ionic Currents ◽  
Voltage Clamp Technique ◽  
Clamp Technique ◽  
Identification And Characterization ◽  
Isolated Smooth Muscle Cells

scholarly journals Histologic and biochemical identification and characterization of an elastin in cartilage.

1977 ◽  
Vol 25 (10) ◽  
pp. 1154-1162 ◽  
Author(s):  
D A Keith ◽  
A Paz ◽  
P M Gallop ◽  
M J Glimcher
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
New Type ◽  
Biochemical Identification ◽  
Chemical Techniques ◽  
Identification And Characterization

Histochemical and chemical techniques have been used to identify, isolate and characterize elastin from certain bovine cartilages. The results strongly suggest that in addition to fibroblasts and smooth muscle cells, chondroblasts also synthesize elastin. Some of the possible functions of elastin in elastic cartilages are discussed and the possiblity of a new type of elastin perhaps unique to cartilage is suggested.

Ionic currents of smooth muscle cells isolated from the ctenophore Mnemiopsis

1988 ◽  
Vol 233 (1271) ◽  
pp. 99-121 ◽  
Keyword(s):  
Smooth Muscle ◽  
Steady State ◽  
Smooth Muscle Cells ◽  
Voltage Clamp ◽  
Muscle Cells ◽  
Ionic Currents ◽  
Patch Clamping ◽  
Excitable Cells ◽  
Inward Current ◽  
Electrode Voltage

The ionic currents of smooth muscle cells isolated from the ctenophore Mnemiopsis were examined by using conventional two-electrode voltage clamp and whole-cell patch clamping methods. Several separable currents were identified. These include: (1) a transient and (2) a steady-state voltage-activated inward current; both are tetrodotoxin (TTX) and saxitoxin (STX) insensitive, partly reduced by decreasing external Ca 2+ or Na + or by addition of 5 mM Co 2+ , D-600 or verapamil and are totally blocked with 5 mM Cd 2+ ; (3) an early, transient, cation-dependent, outward K + current ( I Kca/Na ); (4) a transient, voltage-activated, outward K + current provisionally identified as I A ; (5) a delayed, steady-state, voltage-activated outward K + current ( I K ) and (6) a late, transient, outward K + current which is blocked by Cd 2+ and evident only during long voltage pulses. Despite their phylogenic origin, most of these currents are similar to currents identified in many vertebrate smooth and cardiac muscle preparations, and other excitable cells in higher animals.

Characterization of voltage-gated calcium currents in freshly isolated smooth muscle cells from rat tail main artery

2001 ◽  
Vol 173 (3) ◽  
pp. 257-265 ◽  
Author(s):  
G. V. Petkov ◽  
F. Fusi ◽  
S. Saponara ◽  
H. S. Gagov ◽  
G. P. Sgaragli ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Calcium Currents ◽  
Main Artery ◽  
Voltage Gated ◽  
Isolated Smooth Muscle Cells ◽  
Rat Tail
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