Ca2+ not cyclic AMP mediates the fluid secretory response to isoproterenol in the rat mandibular salivary gland: Whole-cell patch-clamp studies

1988 ◽  
Vol 413 (1) ◽  
pp. 67-76 ◽  
Author(s):  
D. I. Cook ◽  
M. L. Day ◽  
M. P. Champion ◽  
J. A. Young
Keyword(s):  
Salivary Gland ◽  
Cyclic Amp ◽  
Patch Clamp ◽  
Secretory Response ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp

Whole-cell Cl− currents in a human peripheral airway epithelial cell line

1993 ◽  
Vol 71 (9) ◽  
pp. 662-670 ◽  
Author(s):  
Xiaodong Wang ◽  
Ludwik Fedorko ◽  
Yoshinori Marunaka ◽  
Hugh O'Brodovich
Keyword(s):  
Cyclic Amp ◽  
Patch Clamp ◽  
Cell Line ◽  
Pertussis Toxin ◽  
Outward Current ◽  
Patch Clamp Technique ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Peripheral Airway

We have used the whole-cell patch-clamp technique to identify and characterize Cl− currents in a cell line derived from human peripheral airway epithelium (NCI-H-441-4). The permeability sequence and relative selectivity for different anions was Br− (1.4) ~ I− (1.3) > Cl− (1.0) > F− (0.6) > gluconate (0.4) > glutamate (0.2). The current–voltage relationship displayed rectification in the outward direction. Diphenylamine-2-carboxylate (10−4 M) applied intracellularly blocked the outward-rectified current, while extracellularly applied diphenylamine-2-carboxylate had no effect on Cl− current. This current was also blocked by extracellularly applied 5-nitro-2-(3-phenylpropylamino)benzoate (NPPB), with an estimated IC50 of 15.2 μM. Dibutyryl-cyclic AMP (10−4 M) increased outward current, whereas pretreatment with 100 ng/mL pertussis toxin almost completely abolished the Cl− current. Pertussis toxin inhibition of this current could be partially reversed by dialysis of the cell interior with the activated αi–2 subunit of Gi protein. This cell line provides an opportunity to study directly the regulation of Cl− channels in cells derived from the peripheral human lung airways.Key words: chloride secretion, whole-cell patch clamp, GTP binding protein, cyclic AMP, pertussis toxins, 5-nitro-2-(3-phenylpropylamino)benzoate, diphenylamine-2-carboxylate, cell line H441.

Enhanced Monitoring of Nanosecond Electric Pulse-Evoked Membrane Conductance Changes in Whole-Cell Patch Clamp Experiments

2016 ◽  
Vol 249 (5) ◽  
pp. 633-644 ◽  
Author(s):  
Jihwan Yoon ◽  
Normand Leblanc ◽  
Josette Zaklit ◽  
P. Thomas Vernier ◽  
Indira Chatterjee ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Electric Pulse ◽  
Membrane Conductance ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Conductance Changes
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