Sugar transport by brush border membrane vesicles isolated from human small intestine

1978 ◽  
Vol 373 (3) ◽  
pp. 243-248 ◽  
Author(s):  
H. L�cke ◽  
W. Berner ◽  
H. Menge ◽  
H. Murer
Keyword(s):  
Small Intestine ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Sugar Transport ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Human Small Intestine

Preparation and properties of brush-border membrane vesicles from human small intestine

1990 ◽  
Vol 98 (3) ◽  
pp. 676-685 ◽  
Author(s):  
S.P. Shirazi-Beechey ◽  
A.G. Davies ◽  
K. Tebbutt ◽  
J. Dyer ◽  
A. Ellis ◽  
...  
Keyword(s):  
Small Intestine ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Human Small Intestine

Hydrolysis and transglycosylation activities of glycosidases from small intestine brush-border membrane vesicles

2021 ◽  
Vol 139 ◽  
pp. 109940
Author(s):  
Lesbia Cristina Julio-Gonzalez ◽  
F. Javier Moreno ◽  
María Luisa Jimeno ◽  
Elisa G. Doyagüez ◽  
Agustín Olano ◽  
...  
Keyword(s):  
Small Intestine ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles

Role of sodium ion in transport of folic acid in the small intestine

1986 ◽  
Vol 251 (2) ◽  
pp. G218-G222 ◽  
Author(s):  
J. Zimmerman ◽  
J. Selhub ◽  
I. H. Rosenberg
Keyword(s):  
Small Intestine ◽  
Folic Acid ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Test Solution ◽  
Brush Border Membrane Vesicles ◽  
Acid Uptake ◽  
Folate Transport ◽  
Luminal Membrane

The effect of sodium on folate transport across the intestinal luminal membrane was analyzed using two techniques: the "influx" chamber and isolated brush-border membrane vesicles. Preincubation of tissue in Na+-free medium did not have a consistent effect on folic acid influx provided that Na+ was present in the test solution. Replacement of Na+ in the test solution by choline+ resulted in a significant reduction of folic acid influx. However, when intestinal sheets that had been equilibrated in Na+-free solution were exposed to test solution containing either Na+, Li+, K+, Rb+, Cs+, Tris+, or guanidinium+ as main cations, folic acid influx was not significantly decreased. Concentration-dependence studies showed that replacement of Na+ by Rb+ did not affect the saturable mechanism of folate transport. Rather, a decrease in nonsaturable folic acid uptake accounted for the slightly reduced influx observed in the presence of Rb+. Experiments with brush-border membrane vesicles revealed that methotrexate uptake was significantly higher in the presence of external Na+ than in the presence of K+, but was not different from uptake in the presence of K+ plus valinomycin. These data suggest that the saturable component of folate transport is not Na+ dependent, and nonsaturable transport of folic acid across the luminal membrane occurs in part through a conductive pathway that involves a negatively charged species of folate and a cation whose membrane permeability affects the rate of folate transport. The importance of Na+ in this process in vivo derives from the fact that Na+ is the most permeant cation available at the absorptive site in the small intestine.

scholarly journals Transport of thiamine by brush-border membrane vesicles from rat small intestine.

1988 ◽  
Vol 398 (1) ◽  
pp. 329-339 ◽  
Author(s):  
D Casirola ◽  
G Ferrari ◽  
G Gastaldi ◽  
C Patrini ◽  
G Rindi
Keyword(s):  
Small Intestine ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Rat Small Intestine
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