Na+/H+ antiporter properties in peripheral blood lymphocytes from normotensive obese and type 2 diabetic patients do not differ significantly from controls

1992 ◽  
Vol 29 (3-4) ◽  
pp. 237-239 ◽  
Author(s):  
D. Ghigo ◽  
P. Alessio ◽  
S. Burzacca ◽  
C. Costamagna ◽  
G. Anfossi ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Blood Lymphocytes ◽  
Type 2 Diabetic

scholarly journals Rapid Detection of the mt3243A > G Mutation Using Urine Sediment in Elderly Chinese Type 2 Diabetic Patients

2017 ◽  
Vol 2017 ◽  
pp. 1-7
Author(s):  
Yinan Zhang ◽  
Xiujuan Du ◽  
Xinqian Geng ◽  
Chen Chu ◽  
Huijuan Lu ◽  
...  
Keyword(s):  
High Resolution ◽  
Peripheral Blood ◽  
High Resolution Melting ◽  
Diabetic Patients ◽  
Peripheral Blood Leukocytes ◽  
Type 2 Diabetic Patients ◽  
Blood Leukocytes ◽  
Urine Sediment ◽  
Type 2 Diabetic

Objective. In this study, we aimed to identify mt3243A > G mutation carriers in a group of Chinese elderly type 2 diabetic patients by a rapid and noninvasive diagnostic system. Methods. DNA was extracted from blood, saliva, and urine sediment samples. The mutation screening and quantitation of heteroplasmy were performed by high-resolution melting (HRM) curve and pyrosequencing, respectively. Patients with mt3243A > G mutation underwent a detailed audiometric, ophthalmologic, neurological, and cardiac examination. Results. Two patients (2/1041) carrying the mt3243A > G mutation were detected among all type 2 diabetic patients. In patient 1, the heteroplasmy was 0.8%, 2.8%, and 14.7% in peripheral blood leukocytes, saliva, and urine sediment, respectively. In patient 2, the heteroplasmy was 5.3%, 8.4%, and 37.7% in peripheral blood leukocytes, saliva, and urine sediment, respectively. Both of the two patients showed hearing impairment. Abnormal ophthalmologic conditions and hyperintensity on T2-weighted magnetic resonance images were showed in patient 1. Conclusion. The occurrence of mt3243 A > G mutation was 0.2% in Chinese elderly type 2 diabetic patients. Moreover, detection of mt3243 A > G mutation in urine sediment with high-resolution melting (HRM) curve and pyrosequencing is feasible in molecular genetic diagnosis.

scholarly journals VISFATIN LEVEL DISTURBANCES IN PERIPHERAL BLOOD OF PATIENTS WITH DIABETIC NEPHROPATHY

2014 ◽  
pp. 25-30
Author(s):  
I.I. Topchii ◽  
P.S. Semenovykh ◽  
V.YU. Galchinskaya ◽  
N.V. Yefimova
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Plasma ◽  
Peripheral Blood ◽  
Control Group ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Visfatin Level ◽  
Type 2 Diabetic ◽  
In Blood Plasma

 Introduction. Recent studies suggest that visfatin participates in pathogenesis of vascular diabetic complications, in particular diabetic nephropathy (DN). The aim of the present research - definition of visfatin level in peripheral blood of type 2 diabetic patients taking to account renal function disturbances and body mass index (BMI). Materials and methods. 94 type 2 diabetic patients with different stages of DN and 10 healthy subjects (control group) were observed. Visfatin concentration in blood plasma was determined using immunoassay kit. Results. An essential increase of visfatin level in blood plasma took place already in initial stages of the DN. Progressing of the disease was accompanied by more expressed growth of visfatin concentration. In patients with high BMI substantial increase of visfatin level when compared with those with normal IMT was determined. We established strong correlations between visfatin concentrations, urinary albumin levels and blood creatinine concentrations and negative correlations with glomerular filtration rate. Conclusions: Our findings testify that visfatin level displays a functional kidney state and may be used as addition to traditional methods of patients examination.

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