Comparison and improvement of methods for determining soil dehydrogenase activity by using triphenyltetrazolium chloride and iodonitrotetrazolium chloride

1994 ◽  
Vol 18 (4) ◽  
pp. 291-296 ◽  
Author(s):  
J. K. Friedel ◽  
K. M�lter ◽  
W. R. Fischer
Keyword(s):  
Dehydrogenase Activity ◽  
Triphenyltetrazolium Chloride ◽  
Iodonitrotetrazolium Chloride ◽  
Soil Dehydrogenase Activity

scholarly journals Dehydrogenase activity of forest soils depends on the assay used

2015 ◽  
Vol 29 (1) ◽  
pp. 47-59 ◽  
Author(s):  
Kazimierz Januszek ◽  
Joanna Długa ◽  
Jarosław Socha
Keyword(s):  
Dehydrogenase Activity ◽  
Forest Soils ◽  
Chemical Reduction ◽  
Triphenyltetrazolium Chloride ◽  
Ph Values ◽  
Artificial Electron Acceptor ◽  
Original Procedure ◽  
High Concentrations ◽  
Changes In Soil Properties ◽  
Soil Dehydrogenase Activity

Abstract Dehydrogenases are exclusively intracellular enzymes, which play an important role in the initial stages of oxidation of soil organic matter. One of the most frequently used methods to estimate dehydrogenase activity in soil is based on the use of triphenyltetrazolium chloride as an artificial electron acceptor. The purpose of this study was to compare the activity of dehydrogenases of forest soils with varied physicochemical properties using different triphenyltetrazolium chloride assays. The determination was carried out using the original procedure by Casida et al., a modification of the procedure which involves the use of Ca(OH)2 instead of CaCO3, the Thalmann method, and the assay by Casida et al. without addition of buffer or any salt. Soil dehydrogenase activity depended on the assay used. Dehydrogenase determined by the Casida et al. method without addition of buffer or any salt correlated with the pH values of soils. The autoclaved strongly acidic samples of control soils showed high concentrations of triphenylformazan, probably due to chemical reduction of triphenyltetrazolium chloride. There is, therefore, a need for a sterilization method other than autoclaving, ie a process that results in significant changes in soil properties, thus helping to increase the chemical reduction of triphenyltetrazolium chloride.

Grazing enhanced spatial heterogeneity of soil dehydrogenase activity in arid shrublands of Patagonia, Argentina

2019 ◽  
Vol 20 (2) ◽  
pp. 883-888 ◽  
Author(s):  
Magalí S. Marcos ◽  
Analía L. Carrera ◽  
Mónica B. Bertiller ◽  
Nelda L. Olivera
Keyword(s):  
Spatial Heterogeneity ◽  
Dehydrogenase Activity ◽  
Arid Shrublands ◽  
Soil Dehydrogenase Activity

SOIL DEHYDROGENASE ACTIVITY

Soil Science ◽  
1964 ◽  
Vol 98 (6) ◽  
pp. 371-376 ◽  
Author(s):  
L. E. CASIDA ◽  
D. A. KLEIN ◽  
THOMAS SANTORO
Keyword(s):  
Dehydrogenase Activity ◽  
Soil Dehydrogenase Activity

EVALUATION OF A DEHYDROGENASE ASSAY BASED ON TETRAZOLIUM REDUCTION FOR RAPID IN VITRO ESTIMATION OF FERMENTATION ACTIVITY IN RUMEN CONTENTS

1978 ◽  
Vol 58 (3) ◽  
pp. 501-511 ◽  
Author(s):  
G. A. JONES ◽  
B. A. HUMPHREY
Keyword(s):  
Dehydrogenase Activity ◽  
Rumen Fluid ◽  
Stomach Tube ◽  
Triphenyltetrazolium Chloride ◽  
Activity Maximum ◽  
Alfalfa Hay ◽  
The Mean ◽  
Ph Range ◽  
Second Peak

Rumen contents obtained from fistulated steers fed brome–alfalfa hay was used to evaluate a dehydrogenase assay based on the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) to triphenylformazan (TPF) for rapid in vitro estimation of fermentation activity. Maximum absorbance of TPF occurred at 485 nm over the pH range 3–10. TPF formed from TTC was stable to atmospheric O2. Absorbance decreased if TPF was exposed to light. In the presence of excess TTC, production of TPF in strained rumen fluid (SRF) at 39 °C was linear with elapsed incubation times up to at least 5 min. Dehydrogenase activity was abolished in the presence of air. It was significantly increased if the CO2 gas phase above SRF was replaced with N2 or H2, owing, at least in part, to an increase in the pH of the fermentation. The dehydrogenase activity of SRF was maximal at pH 7.8–8.0. Fractionation of SRF by differential centrifugation showed that about 50% of the dehydrogenase activity was associated with a protozoal fraction sedimenting at 188 × g, and 42% with a bacterial fraction sedimenting at 1,475 × g. Neither the cell-free supernatant obtained by centrifuging SRF at 21,500 × g, nor boiled SRF, nor the hay fed to the animals showed dehydrogenase activity. Whole rumen contents (WRC) showed a mean dehydrogenase activity that was 58% higher than that of SRF. The dehydrogenase activity of SRF was increased in the presence of substrates representing major plant carbohydrates, the greatest stimulatory effect being shown by ground brome–alfalfa hay. When rumen contents were sampled at the same time by stomach tube and through the fistula, the mean dehydrogenase activity of SRF prepared from the stomach tube samples was 137% higher than that of SRF prepared from the fistula samples. In an animal fed at 12-h intervals, the dehydrogenase activity of SRF rose within 2 h after feeding, declined for the next 6 h, and showed a second peak at 9 h. Activity then declined to the pre-feeding level. The dehydrogenase activity of WRC showed a similar pattern but the peaks of activity were less pronounced. It was concluded that dehydrogenase activity based on TTC reduction provides a simple and rapid means of estimating the fermentation activity of rumen contents which may reflect the availability of energy substrates to the microbiota.

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