Mapping of a mouse homolog of a Heterochromatin protein gene to the X Chromosome

1991 ◽  
Vol 2 (1) ◽  
pp. 72-75 ◽  
Author(s):  
Renata M. J. Hamvas ◽  
Wolf Reik ◽  
Stephen J. Gaunt ◽  
Stephen D. M. Brown ◽  
Prim B. Singh
Keyword(s):  
X Chromosome ◽  
Protein Gene ◽  
Mouse Homolog ◽  
Heterochromatin Protein

Mapping of two human homologs of a Drosophila heterochromatin protein gene to the X Chromosome

1992 ◽  
Vol 3 (11) ◽  
pp. 650-652 ◽  
Author(s):  
Wolf Reik ◽  
Margaret A. Leversha ◽  
Nick R. Waterfield ◽  
Prim B. Singh
Keyword(s):  
X Chromosome ◽  
Protein Gene ◽  
Heterochromatin Protein ◽  
Drosophila Heterochromatin ◽  
Drosophila Heterochromatin Protein

scholarly journals BRCa1 participates in XIST RNa localization on inactive x chromosome

2019 ◽  
Vol 18 (2) ◽  
pp. 21-26
Author(s):  
E. A. Shestakova ◽  
T. A. Bogush
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Confocal Microscopy ◽  
X Chromosome ◽  
Fluorescent Microscopy ◽  
S Phase ◽  
Immunofluorescent Staining ◽  
Heterochromatin Protein ◽  
Inactive X Chromosome ◽  
Xist Rna

Introduction . Inactive X chromosome (Xi) is associated with noncoding XIST RNA, series of proteins and contains multiple epigenetic modifications that altogether determine a silence of the most of X-linked genes. Recently the data were obtained that tumor suppressor BRCA1 is also associated with Xi. The purpose of this study was to reveal the colocalization of BRCA1 and XIST RNA and precise spatial organization on Xi with the high resolution of confocal microscopy.Materials and methods . The object of the study is IMR90hTERT diploid immortalized fibroblast cell line. For BRCA1 and XIST RNA colocalization analysis on Xi the method of fluorescent hybridization in situ associated with immunofluorescent cell staining (immunoFISH) and confocal microscopy were used. For BRCA1 and heterochromatin protein-1 colocalization study the method of double immunofluorescent staining and common fluorescent microscopy were applied. Results . The study using confocal fluorescent microscopy with higher resolution has demonstrated at first the colocalization of BRCA1 with XIST RNA region of Xi revealed with XIST RNA probes and with replicating Xi and autosomes revealed with BrdU in late S-phase of cell cycle. Altogether, the data obtained suggest the involvement of BRCA1 in the inhibition of gene expression on Xi due to the regulation of XIST RNA association with Xi. Moreover, according to the results of confocal microscopy, BRCA1 also colocalizes with replicating Xi and autosomes revealed with BrdU in late S-phase of cell cycle. This indicates a possible involvement of this protein in the replication of pericentromeric repeats in cellular chromosomes. Colocalization of BRCA1 with heterochromatin protein-1α presented in pericentromeric regions of all chromosomes supports this suggestion.Conclusions . Altogether, the data obtained in this study suggest the involvement of BRCA1 in the inhibition of gene expression on Xi due to the association with noncoding inhibiting XIST RNA and in replication of heterochromatin regions. 

scholarly journals macroH2A1 Histone Variants Are Depleted on Active Genes but Concentrated on the Inactive X Chromosome

2006 ◽  
Vol 26 (12) ◽  
pp. 4410-4420 ◽  
Author(s):  
Lakshmi N. Changolkar ◽  
John R. Pehrson
Keyword(s):  
X Chromosome ◽  
Distribution Patterns ◽  
Detailed Examination ◽  
Histone Variants ◽  
Heterochromatin Protein ◽  
Inactive X Chromosome ◽  
Preferential Localization ◽  
Intergenic Regions ◽  
Liver Chromatin ◽  
Active Genes

ABSTRACT Using a novel thiol affinity chromatography approach to purify macroH2A1-containing chromatin fragments, we examined the distribution of macroH2A1 histone variants in mouse liver chromatin. We found that macroH2A1 was depleted on the transcribed regions of active genes. This depletion was observed on all of the 20 active genes that we probed, with only one site showing a small amount of enrichment. In contrast, macroH2A1 was concentrated on the inactive X chromosome, consistent with our previous immunofluorescence studies. This preferential localization was seen on genes that are active in liver, genes that are inactive in liver, and intergenic regions but was absent from four regions that escape X inactivation. These results support the hypothesis that macroH2As function as transcriptional repressors. Also consistent with this hypothesis is our finding that the heterochromatin protein HP1β copurifies with the macroH2A1-containing chromatin fragments. This study presents the first detailed examination of the distribution of macroH2A1 variants on specific sequences. Our results indicate that macroH2As have complex distribution patterns that are influenced by both local factors and long-range mechanisms.

The Mouse Homolog of the Wiskott–Aldrich Syndrome Protein (WASP) Gene Is Highly Conserved and Maps near the Scurfy (sf) Mutation on the X Chromosome

Genomics ◽  
1995 ◽  
Vol 29 (2) ◽  
pp. 471-477 ◽  
Author(s):  
JONATHAN M.J. DERRY ◽  
PHILIPP WIEDEMANN ◽  
PATRICK BLAIR ◽  
YUKER WANG ◽  
JULIE A. KERNS ◽  
...  
Keyword(s):  
X Chromosome ◽  
Mouse Homolog ◽  
Wiskott Aldrich Syndrome ◽  
Wasp Gene ◽  
Syndrome Protein

scholarly journals Mutation of the proteolipid protein gene PLP in a human X chromosome-linked myelin disorder.

1989 ◽  
Vol 86 (20) ◽  
pp. 8128-8131 ◽  
Author(s):  
L. D. Hudson ◽  
C. Puckett ◽  
J. Berndt ◽  
J. Chan ◽  
S. Gencic
Keyword(s):  
X Chromosome ◽  
Protein Gene ◽  
Proteolipid Protein

scholarly journals Additional Copies of the Proteolipid Protein Gene Causing Pelizaeus-Merzbacher Disease Arise by Separate Integration into the X Chromosome

2000 ◽  
Vol 67 (1) ◽  
pp. 14-22 ◽  
Author(s):  
M.E. Hodes ◽  
Karen Woodward ◽  
Nancy B. Spinner ◽  
Beverly S. Emanuel ◽  
Agnes Enrico-Simon ◽  
...  
Keyword(s):  
X Chromosome ◽  
Protein Gene ◽  
Proteolipid Protein ◽  
Pelizaeus Merzbacher Disease

scholarly journals trans-Acting Factors and cis Elements Involved in the Human Inactive X Chromosome Organization and Compaction

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Zhuo Sun ◽  
Jinbo Fan ◽  
Yufeng Zhao
Keyword(s):  
Dna Methylation ◽  
Histone Modifications ◽  
X Chromosome ◽  
X Chromosome Inactivation ◽  
Chromosome Inactivation ◽  
Chromosome Organization ◽  
Cis Elements ◽  
Heterochromatin Protein ◽  
Inactive X Chromosome ◽  
Covalent Histone Modifications

During X chromosome inactivation, many chromatin changes occur on the future inactive X chromosome, including acquisition of a variety of repressive covalent histone modifications, heterochromatin protein associations, and DNA methylation of promoters. Here, we summarize trans-acting factors and cis elements that have been shown to be involved in the human inactive X chromosome organization and compaction.

Interplay between Pur α and Egr-1 in the transcriptional regulation of amyloid precursor protein gene expression

2010 ◽  
Vol 34 (8) ◽  
pp. S27-S27
Author(s):  
Jianqi Cui ◽  
Xiuying Pei ◽  
Qian Zhang ◽  
Bassel E. Sawaya ◽  
Xiaohong Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Amyloid Precursor Protein ◽  
Protein Gene ◽  
Precursor Protein ◽  
Amyloid Precursor Protein Gene
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