Immunohistochemical evidence for the presence of peptides derived from proenkephalin, prodynorphin and proopiomelanocortin in the guinea pig pineal gland

1988 ◽  
Vol 88 (3-6) ◽  
pp. 333-341 ◽  
Author(s):  
H. Schröder ◽  
E. Weihe ◽  
D. Nohr ◽  
L. Vollrath
Keyword(s):  
Guinea Pig ◽  
Pineal Gland ◽  
Immunohistochemical Evidence

Prenatal development of ?synaptic? ribbons in the guinea pig pineal gland

1990 ◽  
Vol 187 (1) ◽  
pp. 39-54 ◽  
Author(s):  
Z. R. Luo ◽  
R. L. Schultz ◽  
E. F. Whitter ◽  
L. Vollrath
Keyword(s):  
Guinea Pig ◽  
Pineal Gland ◽  
Prenatal Development ◽  
Synaptic Ribbons

PKC δ-isoform translocation and enhancement of tonic contractions of gastrointestinal smooth muscle

2007 ◽  
Vol 292 (3) ◽  
pp. G887-G898 ◽  
Author(s):  
Daniel P. Poole ◽  
John B. Furness
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscles ◽  
Specific Inhibitor ◽  
Smooth Muscle Contraction ◽  
Enteric Neurons ◽  
Guinea Pig Ileum ◽  
Tonic Component ◽  
Gastrointestinal Smooth Muscle ◽  
Immunohistochemical Evidence

PKC is involved in mediating the tonic component of gastrointestinal smooth muscle contraction in response to stimulation by agonists for G protein-coupled receptors. Here, we present pharmacological and immunohistochemical evidence indicating that a member of the novel PKC isoforms, PKC-δ, is involved in maintaining muscarinic receptor-coupled tonic contractions of the guinea pig ileum. The tonic component of carbachol-evoked contractions was enhanced by an activator of conventional and novel PKCs, phorbol 12,13-dibutyrate (PDBu; 200 nM or 1 μM), and by an activator of novel PKCs, ingenol 3,20-dibenzoate (IDB; 100 or 500 nM). Enhancement was unaffected by concentrations of bisindolylmaleimide I (BIM-I; 22 nM) that block conventional PKCs or by a PKC-ε-specific inhibitor peptide but was attenuated by higher doses of BIM-I (2.2 μM). Relevant proteins were localized at a cellular and subcellular level using confocal analysis. Immunohistochemical staining of the ileum showed that PKC-δ was exclusively expressed in smooth muscles distributed throughout the layers of the gut wall. PKC-ε immunoreactivity was prominent in enteric neurons but was largely absent from smooth muscle of the muscularis externa. Treatment with PDBu, IDB, or carbachol resulted in a time- and concentration-dependent translocation of PKC-δ from the cytoplasm to filamentous structures within smooth muscle cells. These were parallel to, but distinct from, actin filaments. The translocation of PKC-δ in response to carbachol was significantly reduced by scopolamine or calphostin C. The present study indicates that the tonic carbachol-induced contraction of the guinea pig ileum is mediated through a novel PKC, probably PKC-δ.

Immunohistochemical evidence for the presence of calbindin containing neurones in the myenteric plexus of the guinea-pig stomach

1999 ◽  
Vol 270 (2) ◽  
pp. 71-74 ◽  
Author(s):  
Dania Reiche ◽  
Helga Pfannkuche ◽  
Klaus Michel ◽  
Susanne Hoppe ◽  
Michael Schemann
Keyword(s):  
Guinea Pig ◽  
Myenteric Plexus ◽  
Immunohistochemical Evidence ◽  
Guinea Pig Stomach

Immunohistochemical evidence for the presence of melatonin in the pineal gland, the retina and the Harderian gland

1981 ◽  
Vol 217 (1) ◽  
Author(s):  
B. Vivien-Roels ◽  
P. P�vet ◽  
M.P. Dubois ◽  
J. Arendt ◽  
G.M. Brown
Keyword(s):  
Pineal Gland ◽  
Harderian Gland ◽  
Immunohistochemical Evidence
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