The globulins of cotton seeds XV. Primary structure of a glycopeptide of subunit C11 of the S-globulin

1978 ◽  
Vol 14 (4) ◽  
pp. 467-468
Author(s):  
S. I. Asatov ◽  
T. S. Yunusov ◽  
P. Kh. Yuldashev
Keyword(s):  
Primary Structure ◽  
Cotton Seeds

The globulins of cotton seeds XVII. Primary structure of the carbohydrate-containing subunit C of the 11S globulin

1978 ◽  
Vol 14 (4) ◽  
pp. 470-471 ◽  
Author(s):  
S. I. Asatov ◽  
É. G. Yadgarov ◽  
T. S. Yunusov ◽  
P. Kh. Yuldashev
Keyword(s):  
Primary Structure ◽  
Subunit C ◽  
Cotton Seeds ◽  
11S Globulin

Warm and freeze-fracture of cotton seed radicles

1987 ◽  
Vol 45 ◽  
pp. 984-985
Author(s):  
E. L. Vigil ◽  
E. F. Erbe
Keyword(s):  
Thin Film ◽  
Water Vapor ◽  
Fracture Surface ◽  
Membrane Structure ◽  
Room Temperature ◽  
Freeze Fracture ◽  
Longitudinal Axis ◽  
Fracture Plane ◽  
Cotton Seeds ◽  
Condensed Water

In cotton seeds the radicle has 12% moisture content which makes it possible to prepare freeze-fracture replicas without fixation or cryoprotection. For this study we have examined replicas of unfixed radicle tissue fractured at room temperature to obtain data on organelle and membrane structure.Excised radicles from seeds of cotton (Gossyplum hirsutum L. M-8) were fractured at room temperature along the longitudinal axis. The fracture was initiated by spliting the basal end of the excised radicle with a razor. This procedure produced a fracture through the tissue along an unknown fracture plane. The warm fractured radicle halves were placed on a thin film of 100% glycerol on a flat brass cap with fracture surface up. The cap was rapidly plunged into liquid nitrogen and transferred to a freeze- etch unit. The sample was etched for 3 min at -95°C to remove any condensed water vapor and then cooled to -150°C for platinum/carbon evaporation.

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