The role of the N- and C-terminal amino acids of activated peptide esters in their polycondensation

1974 ◽  
Vol 10 (4) ◽  
pp. 504-507
Author(s):  
A. B. Zegel'man ◽  
S. V. Alieva ◽  
G. N. Demyanik ◽  
V. I. Ul'yanova ◽  
O. D. Turaev
Keyword(s):  
Amino Acids ◽  
Terminal Amino ◽  
Peptide Esters

scholarly journals Roles of the C-Terminal Amino Acids of Non-Hexameric Helicases: Insights from Escherichia coli UvrD

2021 ◽  
Vol 22 (3) ◽  
pp. 1018
Author(s):  
Hiroaki Yokota
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Amino Acid ◽  
Single Molecule ◽  
Underlying Mechanism ◽  
Amino Acid Sequences ◽  
E Coli ◽  
X Ray Crystallography ◽  
Terminal Amino

Helicases are nucleic acid-unwinding enzymes that are involved in the maintenance of genome integrity. Several parts of the amino acid sequences of helicases are very similar, and these quite well-conserved amino acid sequences are termed “helicase motifs”. Previous studies by X-ray crystallography and single-molecule measurements have suggested a common underlying mechanism for their function. These studies indicate the role of the helicase motifs in unwinding nucleic acids. In contrast, the sequence and length of the C-terminal amino acids of helicases are highly variable. In this paper, I review past and recent studies that proposed helicase mechanisms and studies that investigated the roles of the C-terminal amino acids on helicase and dimerization activities, primarily on the non-hexermeric Escherichia coli (E. coli) UvrD helicase. Then, I center on my recent study of single-molecule direct visualization of a UvrD mutant lacking the C-terminal 40 amino acids (UvrDΔ40C) used in studies proposing the monomer helicase model. The study demonstrated that multiple UvrDΔ40C molecules jointly participated in DNA unwinding, presumably by forming an oligomer. Thus, the single-molecule observation addressed how the C-terminal amino acids affect the number of helicases bound to DNA, oligomerization, and unwinding activity, which can be applied to other helicases.

Role of N- and C-Terminal Amino Acids in Two-Dimensional Streptavidin Crystal Formation

Langmuir ◽  
2000 ◽  
Vol 16 (11) ◽  
pp. 5199-5204 ◽  
Author(s):  
Szu-Wen Wang ◽  
Channing Robertson ◽  
Alice Gast ◽  
Sandy Koppenol ◽  
Todd Edwards ◽  
...  
Keyword(s):  
Amino Acids ◽  
Crystal Formation ◽  
Two Dimensional ◽  
Terminal Amino

Aminopeptidase Activity Profile in Cultured Human Osteoblasts

2011 ◽  
Vol 15 (1) ◽  
pp. 56-61 ◽  
Author(s):  
Gema Lucena ◽  
Candela Reyes-Botella ◽  
Olga García-Martínez ◽  
Lourdes Díaz-Rodríguez ◽  
Francisco Alba ◽  
...  
Keyword(s):  
Amino Acids ◽  
Bone Tissue ◽  
The Other ◽  
Aminopeptidase Activity ◽  
Cell Populations ◽  
Biological Processes ◽  
Activity Profile ◽  
Human Osteoblasts ◽  
Terminal Amino

Aminopeptidases (APs) are enzymes involved in a wide variety of biological processes and present in a variety of different cell populations. The authors studied these enzymes in primary cultured human osteoblasts in order to establish an activity profile and thereby contribute to knowledge of bone tissue. The authors used 13 different substrates ( N-terminal amino acids) and a fluorimetric assay to examine AP activity associated with the membranes of cultured osteoblasts. The authors demonstrated activity > 10 pmol/min/104 cells when glycine, alanine, leucine, arginine, phenylalanine, methionine, and lysine were used as substrates. The activity was markedly lower (<1.6 pmol/min/104 cells) when the other N-terminal amino acids were used. Puromycin and bestatin inhibited AP activity, though not completely, when we used AlaNA or LeuNA as substrates. Further studies are warranted to determine the role of these enzymes in bone tissue physiology.

Role Of The C-Terminal Amino Acids In Beta2-Microglobulin Amyloid Formation

2007 ◽  
pp. 16-18
Author(s):  
M Ueda ◽  
J Kim ◽  
Y Motomiya ◽  
U Semba ◽  
Y Ando ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amyloid Formation ◽  
Terminal Amino

scholarly journals Role of N-Terminal Amino Acids in the Potency of Anthrax Lethal Factor

PLoS ONE ◽  
2008 ◽  
Vol 3 (9) ◽  
pp. e3130 ◽  
Author(s):  
Pradeep K. Gupta ◽  
Mahtab Moayeri ◽  
Devorah Crown ◽  
Rasem J. Fattah ◽  
Stephen H. Leppla
Keyword(s):  
Amino Acids ◽  
Lethal Factor ◽  
Anthrax Lethal Factor ◽  
Terminal Amino

scholarly journals Role of N- and C-terminal amino acids in antithrombin binding to pentasaccharide.

1994 ◽  
Vol 269 (47) ◽  
pp. 29435-29443
Author(s):  
B Mille ◽  
J Watton ◽  
T W Barrowcliffe ◽  
J C Mani ◽  
D A Lane
Keyword(s):  
Amino Acids ◽  
Terminal Amino
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