Quantitative thin-layer chromatographic determination of dexamethasone and dexamethasone sodium hydrogen sulfate in blood and in pharmaceutical preparations

1988 ◽  
Vol 329 (7) ◽  
pp. 778-780 ◽  
Author(s):  
M. Amin
Keyword(s):  
Thin Layer ◽  
Pharmaceutical Preparations ◽  
Chromatographic Determination ◽  
Hydrogen Sulfate ◽  
Sodium Hydrogen

High-performance thin-layer chromatographic determination of rosiglitazone in its dosage form

2002 ◽  
Vol 15 (3) ◽  
pp. 192-195 ◽  
Author(s):  
Ramesh Sane ◽  
Mary Francis ◽  
Atul Moghe ◽  
Sachin Khedkar ◽  
Ajit Anerao
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Dosage Form ◽  
Chromatographic Determination

scholarly journals Application of Derivative, Derivative Ratio, and Multivariate Spectral Analysis and Thin-Layer Chomatography-Densitometry for Determination of a Ternary Mixture Containing Drotaverine Hydrochloride, Caffeine, and Paracetamol

2007 ◽  
Vol 90 (2) ◽  
pp. 391-404 ◽  
Author(s):  
Fadia H Metwally ◽  
Yasser S El-Saharty ◽  
Mohamed Refaat ◽  
Sonia Z El-Khateeb
Keyword(s):  
Thin Layer ◽  
Least Squares ◽  
Ternary Mixture ◽  
Principal Component Regression ◽  
Pharmaceutical Preparations ◽  
Principal Component ◽  
Standard Addition ◽  
Drotaverine Hydrochloride ◽  
Assay Precision

Abstract New selective, precise, and accurate methods are described for the determination of a ternary mixture containing drotaverine hydrochloride (I), caffeine (II), and paracetamol (III). The first method uses the first (D1) and third (D3) derivative spectrophotometry at 331 and 315 nm for the determination of (I) and (III), respectively, without interference from (II). The second method depends on the simultaneous use of the first derivative of the ratio spectra (DD1) with measurement at 312.4 nm for determination of (I) using the spectrum of 40 μg/mL (III) as a divisor or measurement at 286.4 and 304 nm after using the spectrum of 4 μg/mL (I) as a divisor for the determination of (II) and (III), respectively. In the third method, the predictive abilities of the classical least-squares, principal component regression, and partial least-squares were examined for the simultaneous determination of the ternary mixture. The last method depends on thin-layer chromatography-densitometry after separation of the mixture on silica gel plates using ethyl acetatechloroformmethanol (16 + 3 + 1, v/v/v) as the mobile phase. The spots were scanned at 281, 272, and 248 nm for the determination of (I), (II), and (III), respectively. Regression analysis showed good correlation in the selected ranges with excellent percentage recoveries. The chemical variables affecting the analytical performance of the methodology were studied and optimized. The methods showed no significant interferences from excipients. Intraday and interday assay precision and accuracy values were within regulatory limits. The suggested procedures were checked using laboratory-prepared mixtures and were successfully applied for the analysis of their pharmaceutical preparations. The validity of the proposed methods was further assessed by applying a standard addition technique. The results obtained by applying the proposed methods were statistically analyzed and compared with those obtained by the manufacturer's method.

Improved thin-layer chromatographic determination of phospholipids in gastric aspirate from newborns, for assessment of lung maturity.

1988 ◽  
Vol 34 (4) ◽  
pp. 736-738 ◽  
Author(s):  
D Serrano de la Cruz ◽  
E Santillana ◽  
A Mingo ◽  
G Fuenmayor ◽  
A Pantoja ◽  
...  
Keyword(s):  
Thin Layer ◽  
Fetal Lung ◽  
Chromatographic Determination ◽  
Solvent Mixture ◽  
Gastric Aspirate ◽  
One Dimensional ◽  
Acetic Acid Water ◽  
Chromatographic Resolution ◽  
Lung Maturity

Abstract This one-dimensional thin-layer chromatographic method is used for assay of phospholipids in the gastric aspirate of newborns. The solvent mixture (chloroform/hexane/methanol/glacial acetic acid/water, 12/7/4/3/0.3 by vol) completely resolves lecithin, sphingomyelin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol. The method is simple, precise, inexpensive, and rapid (chromatographic development takes less than 25 min) and gives high chromatographic resolution. We used this method to determine the lecithin/sphingomyelin densitometric ratio (L/S ratio) and the phosphatidylglycerol percentage in 200 samples of gastric aspirate and found an L/S ratio of 2.5 to be a satisfactory cutoff value for distinguishing fetal lung maturity and immaturity. We confirmed that the presence of phosphatidylglycerol excluded the possibility of respiratory distress.

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