Semiempirical description of the C6H5N reactive intermediate in phenyl azide photolysis

1976 ◽  
Vol 43 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Donald D. Shillady ◽  
Carl Trindle
Keyword(s):  
Reactive Intermediate ◽  
Phenyl Azide

Site-Specific Protein Covalent Attachment to Nanotubes and Its Electronic Impact on Single Molecule Function

2019 ◽  
Author(s):  
Adam Beachey ◽  
Harley Worthy ◽  
William David Jamieson ◽  
Suzanne Thomas ◽  
Benjamin Bowen ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescent Protein ◽  
Functional Integration ◽  
Attachment Site ◽  
Covalent Attachment ◽  
Great Promise ◽  
Functional Coupling ◽  
Phenyl Azide ◽  
Electronic Impact ◽  
Protein Attachment

<p>Functional integration of proteins with carbon-based nanomaterials such as nanotubes holds great promise in emerging electronic and optoelectronic applications. Control over protein attachment poses a major challenge for consistent and useful device fabrication, especially when utilizing single/few molecule properties. Here, we exploit genetically encoded phenyl azide photochemistry to define the direct covalent attachment of three different proteins, including the fluorescent protein GFP, to carbon nanotube side walls. Single molecule fluorescence revealed that on attachment to SWCNTs GFP’s fluorescence changed in terms of intensity and improved resistance to photobleaching; essentially GFP is fluorescent for much longer on attachment. The site of attachment proved important in terms of electronic impact on GFP function, with the attachment site furthest from the functional center having the larger effect on fluorescence. Our approach provides a versatile and general method for generating intimate protein-CNT hybrid bioconjugates. It can be potentially applied easily to any protein of choice; attachment position and thus interface characteristics with the CNT can easily be changed by simply placing the phenyl azide chemistry at different residues by gene mutagenesis. Thus, our approach will allow consistent construction and modulate functional coupling through changing the protein attachment position.</p>

Mechanism of the propagation step in the anionic polymerization of styrene

1980 ◽  
Vol 45 (4) ◽  
pp. 1047-1055 ◽  
Author(s):  
Miroslav Kašpar ◽  
Jiří Trekoval
Keyword(s):  
Experimental Data ◽  
Induction Period ◽  
Anionic Polymerization ◽  
Reactive Intermediate ◽  
Coordination Polymerization ◽  
Kinetic Dependence ◽  
Mathematical Solution ◽  
Slow Reaction

The paper is dealing with an investigation of the kinetic dependence of the propagation step in the anionic coordination polymerization of styrene in benzene at 303 K with "living" oligostyryllithium as initiator at the onset of the reaction. A short but distinct induction period was found, indicating a preceding slow reaction leading to the formation of a reactive intermediate, which behaves as the initiator of the reaction. Using results obtained in the first paper of this series, a new mechanism of propagation has been suggested, the mathematical solution of which is correlated with experimental data.

scholarly journals Evidence for a Negative Correlation between Human Reactive Enamine-Imine Intermediate Deaminase A (RIDA) Activity and Cell Proliferation Rate: Role of Lysine Succinylation of RIDA

2021 ◽  
Vol 22 (8) ◽  
pp. 3804
Author(s):  
Luisa Siculella ◽  
Laura Giannotti ◽  
Benedetta Di Chiara Stanca ◽  
Matteo Calcagnile ◽  
Alessio Rochira ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Lines ◽  
Negative Correlation ◽  
Cancer Biology ◽  
Human Tumor ◽  
In Silico Analysis ◽  
Proliferation Rate ◽  
Reactive Intermediate ◽  
Cell Proliferation Rate

Reactive intermediate deaminase (Rid) proteins are enzymes conserved in all domains of life. UK114, a mammalian member of RidA subfamily, has been firstly identified as a component of liver perchloric acid-soluble proteins (L-PSP). Although still poorly defined, several functions have been attributed to the mammalian protein UK114/RIDA, including the reactive intermediate deamination activity. The expression of UK114/RIDA has been observed in some tumors, arousing interest in this protein as an evaluable tumor marker. However, other studies reported a negative correlation between UK114/RIDA expression, tumor differentiation degree and cell proliferation. This work addressed the question of UK114/RIDA expression in human non-tumor HEK293 cell lines and in some human tumor cell lines. Here we reported that human RIDA (hRIDA) was expressed in all the analyzed cell line and subjected to lysine (K-)succinylation. In HEK293, hRIDA K-succinylation was negatively correlated to the cell proliferation rate and was under the control of SIRT5. Moreover, K-succinylation clearly altered hRIDA quantification by immunoblotting, explaining, at least in part, some discrepancies about RIDA expression reported in previous studies. We found that hRIDA was able to deaminate reactive enamine-imine intermediates and that K-succinylation drastically reduced deaminase activity. As predicted by in silico analysis, the observed reduction of deaminase activity has been related to the drastic alterations of hRIDA structure inferred by K-succinylation. The role of hRIDA and the importance of its K-succinylation in cell metabolism, especially in cancer biology, have been discussed.

Carbene radical anions. New species of reactive intermediate

1972 ◽  
Vol 94 (20) ◽  
pp. 7174-7176 ◽  
Author(s):  
G. Dann Sargent ◽  
Charles M. Tatum ◽  
Scott M. Kastner
Keyword(s):  
New Species ◽  
Radical Anions ◽  
Reactive Intermediate

Reaction of o-phenylenediamine with dibenzoylethylene and dibenzoylacetylene. isolation of a reactive intermediate

1975 ◽  
Vol 16 (25) ◽  
pp. 2073-2076 ◽  
Author(s):  
R.G. Bass ◽  
D.D. Crichton ◽  
Harriet K. Meetz ◽  
Ashby F. Johnson
Keyword(s):  
Reactive Intermediate

Mechanism for the direct synthesis of tryptophan from indole and serine: a useful NMR technique for the detection of a reactive intermediate in the reaction mixture

2010 ◽  
Vol 48 (10) ◽  
pp. 811-817 ◽  
Author(s):  
Yuusaku Yokoyama ◽  
Masamichi Nakakoshi ◽  
Hiroaki Okuno ◽  
Yohko Sakamoto ◽  
Satoshi Sakurai
Keyword(s):  
Direct Synthesis ◽  
Reactive Intermediate
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