Presence of nucleoside triphosphates and calcium associated with mycobacteriophage I3

1981 ◽  
Vol 130 (1) ◽  
pp. 50-53 ◽  
Author(s):  
S. Sadashiva Karnik ◽  
K. P. Gopinathan
Keyword(s):  
Nucleoside Triphosphates ◽  
Mycobacteriophage I3

Recent Advances in the Syntheses of Nucleoside Triphosphates

2014 ◽  
Vol 10 (6) ◽  
pp. 903-934 ◽  
Author(s):  
Anilkumar Kore ◽  
Balasubramanian Srinivasan
Keyword(s):  
Recent Advances ◽  
Nucleoside Triphosphates

New nucleoside triphosphates modified at 5'-position, synthesis and substrate properties towards DNA polymerases

1993 ◽  
Vol 58 (s1) ◽  
pp. 120-121
Author(s):  
E. Shirokova ◽  
A. Shipitsin ◽  
E. Kusnetsova ◽  
L. Victorova ◽  
A. Krayevsky
Keyword(s):  
Dna Polymerases ◽  
Nucleoside Triphosphates ◽  
Substrate Properties ◽  
Position Synthesis

scholarly journals Nucleoside Triphosphates as Precursors of Ribonucleic Acid End Groups in a Mammalian System

1958 ◽  
Vol 233 (4) ◽  
pp. 954-963 ◽  
Author(s):  
Liselotte I. Hecht ◽  
Paul C. Zamecnik ◽  
Mary L. Stephenson ◽  
Jesse F. Scott
Keyword(s):  
Ribonucleic Acid ◽  
Nucleoside Triphosphates ◽  
End Groups ◽  
Mammalian System

scholarly journals All1371 is a polyphosphate-dependent glucokinase in Anabaena sp. PCC 7120

Microbiology ◽  
2014 ◽  
Vol 160 (12) ◽  
pp. 2807-2819 ◽  
Author(s):  
Friederike Klemke ◽  
Gabriele Beyer ◽  
Linda Sawade ◽  
Ali Saitov ◽  
Thomas Korte ◽  
...  
Keyword(s):  
Divalent Cations ◽  
Enzymic Activity ◽  
Phosphoryl Group ◽  
Nitrogen Deprivation ◽  
Ping Pong ◽  
Nucleoside Triphosphates ◽  
Anabaena Sp ◽  
Pcc 7120 ◽  
Promoter Studies

The polyphosphate glucokinases can phosphorylate glucose to glucose 6-phosphate using polyphosphate as the substrate. ORF all1371 encodes a putative polyphosphate glucokinase in the filamentous heterocyst-forming cyanobacterium Anabaena sp. PCC 7120. Here, ORF all1371 was heterologously expressed in Escherichia coli, and its purified product was characterized. Enzyme activity assays revealed that All1371 is an active polyphosphate glucokinase that can phosphorylate both glucose and mannose in the presence of divalent cations in vitro. Unlike many other polyphosphate glucokinases, for which nucleoside triphosphates (e.g. ATP or GTP) act as phosphoryl group donors, All1371 required polyphosphate to confer its enzymic activity. The enzymic reaction catalysed by All1371 followed classical Michaelis–Menten kinetics, with k cat = 48.2 s−1 at pH 7.5 and 28 °C and K M = 1.76 µM and 0.118 mM for polyphosphate and glucose, respectively. Its reaction mechanism was identified as a particular multi-substrate mechanism called the ‘bi-bi ping-pong mechanism’. Bioinformatic analyses revealed numerous polyphosphate-dependent glucokinases in heterocyst-forming cyanobacteria. Viability of an Anabaena sp. PCC 7120 mutant strain lacking all1371 was impaired under nitrogen-fixing conditions. GFP promoter studies indicate expression of all1371 under combined nitrogen deprivation. All1371 might play a substantial role in Anabaena sp. PCC 7120 under these conditions.

ChemInform Abstract: Synthesis of Some Mimics of Nucleoside Triphosphates.

ChemInform ◽  
2010 ◽  
Vol 22 (43) ◽  
pp. no-no
Author(s):  
D. M. COE ◽  
H. HILPERT ◽  
S. A. NOBLE ◽  
M. R. PEEL ◽  
S. M. ROBERTS ◽  
...  
Keyword(s):  
Nucleoside Triphosphates
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