Uptake of methylamine via an inducible, energy-dependent transport system in the facultative methylotroph Arthrobacter P1

1982 ◽  
Vol 133 (4) ◽  
pp. 261-266 ◽  
Author(s):  
Lubbert Dijkhuizen ◽  
Lex de Boer ◽  
Roelof H. Boers ◽  
Wim Harder ◽  
Wil N. Konings
Keyword(s):  
Transport System ◽  
Arthrobacter P1 ◽  
Facultative Methylotroph ◽  
Dependent Transport ◽  
Energy Dependent

Citrate transport in Salmonella typhimurium: studies with 2-fluoro-L-erythro-citrate as a substrate

1980 ◽  
Vol 58 (10) ◽  
pp. 797-803 ◽  
Author(s):  
D. M. Ashton ◽  
G. D. Sweet ◽  
J. M. Somers ◽  
W. W. Kay
Keyword(s):  
Salmonella Typhimurium ◽  
Transport System ◽  
Protein C ◽  
Competitive Inhibitor ◽  
C Protein ◽  
High Affinity ◽  
Citrate Transport ◽  
Resistant Mutants ◽  
Dependent Transport ◽  
Energy Dependent

The citrate analogue, 2-fluoro-L-erythro-[3,4,5,6-14C]citrate was synthesized as a probe for the citrate transport system of Salmonella typhimurium. This analogue was actively transported by an inducible energy-dependent transport system with high affinity for fluorocitrate (Km = 3.3 μM), and this transport system was inhibited competitively by citrate and isocitrate. Fluorocitrate was shown to be a competitive inhibitor of the citrate-binding protein (C protein) of this organism (Ki = 4–5 μM). Analogue resistant mutants were simultaneously defective in fluorocitrate transport as well as the C protein and the affected allele, tctC, was located at 59 units on the S. typhimurium chromosome map. These tctC mutants were shown to be specifically defective in K+-dependent fluorocitrate transport but still retained another system capable of transporting fluorocitrate in the presence of both Na+ and K+.

scholarly journals The ß-Glucosidase of the Yeast Cell Surface

1966 ◽  
Vol 50 (1) ◽  
pp. 9-24 ◽  
Author(s):  
J. Gordin Kaplan ◽  
Wanda Tacreiter
Keyword(s):  
Transport System ◽  
Metabolic Energy ◽  
Uptake System ◽  
Entire System ◽  
Membrane Component ◽  
Intact Cells ◽  
Yeast Cell Surface ◽  
Dependent Transport ◽  
Energy Dependent ◽  
Rate Limiting

There are two distinct components of the system which limits the rate at which intact cells of S. cerevisiae C hydrolyze external ß-glucosides; one component requires metabolic energy and the other is stereospecific for ß-glucosides. The stereospecific component is localized at the cell membrane, as shown by its sensitivity to heavy metal inhibitors which did not penetrate the cell under the conditions used. It was shown that cellobiose-grown cells were able to remove cellobiose from the medium in which they were incubated, and that the cellobiose uptake system was identical to that which limits the patent ß-glucosidase activity. In order to test the hypothesis that the system in question was a transport system, for ß-glucosides the ability of cellobiose-grown cells to take up 14C-labeled methyl-ß-glucoside (MBG) was studied. The induced cells were able to take up MBG-14C and the label could be partially chased out by cold MBG and cellobiose; glucose-grown cells could not incorporate label. However, induced cells could not take up label when incubated with 14C-MBG, thus excluding the hypothesis of transport of intact ß-glucosides. It was concluded that the stereospecific membrane component was actually a ß-glucosidase, coupled to an energy-dependent transport system for the glucose moiety; the function of the latter was rate-limiting in the over-all activity of the entire system.

scholarly journals Periplasmic binding protein dependent transport system for maltose and maltodextrins: some recent studies

1989 ◽  
Vol 63 (1-2) ◽  
pp. 53-60
Author(s):  
W. Saurin ◽  
E. Francoz ◽  
P. Martineau ◽  
A. Charbit ◽  
E. Dassa ◽  
...  
Keyword(s):  
Transport System ◽  
Binding Protein ◽  
Periplasmic Binding Protein ◽  
Dependent Transport

A dual, concentration-dependent transport system for riboflavin in rat intestine in vitro

1985 ◽  
Vol 5 (11) ◽  
pp. 1269-1279 ◽  
Author(s):  
Hamid M. Said ◽  
Daniel Hollander ◽  
Yen Duong
Keyword(s):  
Transport System ◽  
Rat Intestine ◽  
Dependent Transport

Energy dependent transport length scales in strongly diffusive carbon nanotubes

2006 ◽  
Vol 18 (19) ◽  
pp. 4581-4587 ◽  
Author(s):  
B Lassagne ◽  
B Raquet ◽  
J M Broto ◽  
J González
Keyword(s):  
Carbon Nanotubes ◽  
Length Scales ◽  
Transport Length ◽  
Dependent Transport ◽  
Energy Dependent

Mechanism of TonB-dependent transport system in Halomonas alkalicola CICC 11012s in response to alkaline stress

Extremophiles ◽  
2020 ◽  
Author(s):  
Lei Zhai ◽  
Jiuyan Xie ◽  
Huijun Feng ◽  
Sijia Sun ◽  
Kun Cheng ◽  
...  
Keyword(s):  
Transport System ◽  
Alkaline Stress ◽  
Dependent Transport
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