A quantitative histochemical technique for the characterisation of ?-glucosidases in the brush-border membrane of rat jejunum

1979 ◽  
Vol 63 (1) ◽  
pp. 81-101 ◽  
Author(s):  
S. Gutschmidt ◽  
W. Kaul ◽  
E. O. Riecken
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Jejunum ◽  
Histochemical Technique

Enhancement of brush border membrane peptidase activity in rat jejunum induced by starvation

2000 ◽  
Vol 440 (1) ◽  
pp. 75 ◽  
Author(s):  
Takashi Ihara ◽  
Tomoyuki Tsujikawa ◽  
Yoshihide Fujiyama ◽  
Hisao Ueyama ◽  
Iwao Ohkubo ◽  
...  
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Peptidase Activity ◽  
Rat Jejunum

Transport of Methionine Hydroxy Analog across the Brush Border Membrane of Rat Jejunum

1987 ◽  
Vol 117 (7) ◽  
pp. 1241-1246 ◽  
Author(s):  
Patrick Brachet ◽  
Antoine Puigserver
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Jejunum

Ontogenesis of intestinal taurine transport: evidence for a beta-carrier in developing rat jejunum

1988 ◽  
Vol 254 (6) ◽  
pp. G870-G877 ◽  
Author(s):  
M. S. Moyer ◽  
A. L. Goodrich ◽  
M. M. Rolfes ◽  
F. J. Suchy
Keyword(s):  
Amino Acids ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Equilibrium Concentration ◽  
Membrane Vesicles ◽  
Transport Systems ◽  
Rat Jejunum ◽  
Adult Rats ◽  
Taurine Transport ◽  
Taurine Uptake

Taurine, a sulfur-containing beta-amino acid, may be conditionally essential during development. However, the existence of a carrier system for beta-amino acids has not been demonstrated in brush-border membrane vesicles (BBMV) from adult rat jejunum. We studied the uptake of [3H]taurine in BBMV prepared from the jejunum of developing and adult rats using a cation-precipitation technique. Uptake of 10 microM [3H]taurine by adult BBMV was slightly enhanced in the presence of an inwardly directed 100 mM Na+ gradient compared with a K+ gradient, and there was no intravesicular accumulation of isotope above the equilibrium concentration ("overshoot"). In contrast, taurine transport by BBMV from 10-day-old rat pups was markedly accelerated in the presence of a Na+ gradient compared with a K+ gradient and a twofold overshoot was observed. Na+-dependent taurine uptake was inhibited by the structural analogues hypotaurine and beta-alanine but not by alpha-alanine or glutamine, which are amino acids served by other transport systems. By computer analysis, Na+-dependent taurine uptake (2-1,000 microM) was saturable with an apparent Km of 74.80 +/- 11.87 microM and a Vmax of 53.55 +/- 2.76 pmol.mg protein-1.min-1. With increasing postnatal age, there was a marked decrease in the initial rate and peak intravesicular accumulation of taurine with disappearance of the overshoot by 21 days of age. We conclude 1) a Na+-dependent carrier mechanism for taurine transport is present in the brush-border membrane of suckling rat jejunum and 2) the activity of this carrier decreases after weaning.(ABSTRACT TRUNCATED AT 250 WORDS)

Effects of taurodeoxycholate on in vivo water and solute transport in rat jejunum in absence and presence of calcium

1986 ◽  
Vol 250 (2) ◽  
pp. G248-G251
Author(s):  
H. V. Ammon ◽  
D. S. Cho ◽  
R. L. Loeffler ◽  
K. L. Reetz
Keyword(s):  
Fatty Acids ◽  
Bile Acids ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Calcium Absorption ◽  
Intestinal Transport ◽  
Fluid Secretion ◽  
Rat Jejunum ◽  
Perfusion Studies

Bile acids and fatty acids enhance the permeability of brush-border membrane vesicles for calcium. It has been postulated that increased influx of calcium into the enterocyte might be responsible for the fluid secretion induced by dihydroxy bile acids and fatty acids. During in vivo perfusion studies of the rat jejunum, 15 mM taurodeoxycholate induced secretion of electrolytes and water (P less than 0.001), reduced glucose absorption (P less than 0.001), and enhanced the absorption of mannitol (P less than 0.0125) and calcium (P less than 0.001). Calcium absorption continued to be enhanced during perfusion of a CaCl2-containing solution following the perfusion with taurodeoxycholate (P less than 0.05). In view of the previously demonstrated enhanced permeability of the apical brush-border membrane in the presence of bile acids, it is very likely that some calcium enters the enterocyte along the steep concentration gradient in the presence of taurodeoxycholate. In spite of enhanced calcium absorption, 15 mM CaCl2 had no effect on control absorption rates or on fluid secretion induced by taurodeoxycholate. The data indicate that the effects of bile acids on intestinal transport are not mediated by an influx of calcium into the enterocyte.

scholarly journals Rapid insertion of GLUT2 into the rat jejunal brush-border membrane promoted by glucagon-like peptide 2

2002 ◽  
Vol 367 (1) ◽  
pp. 247-254 ◽  
Author(s):  
Anita AU ◽  
Alina GUPTA ◽  
Paul SCHEMBRI ◽  
Chris. I. CHEESEMAN
Keyword(s):  
Membrane Proteins ◽  
Western Blotting ◽  
Brush Border ◽  
Brush Border Membrane ◽  
High Capacity ◽  
Intestinal Lumen ◽  
Rat Jejunum ◽  
Frozen Sections ◽  
Glucagon Like Peptide

A possible role for GLUT2 transiently expressed in the rat jejunal brush-border membrane (BBM) under the influence of glucagon-like peptide 2 (GLP-2) was investigated using in vivo perfusion of the intestinal lumen as well as isolation of membrane proteins and immunohistochemistry. A 1h vascular infusion of GLP-2 in vivo doubled the rate of fructose absorption and this increase could be blocked by luminal phloretin. Immunohistochemistry of frozen sections of rat jejunum showed the expression of GLUT2 in both the basolateral and BBMs of mature enterocytes. Perfusion of the intestinal lumen with 50mM d-glucose or vascular infusion of 800pM GLP-2 for 1h increased the expression of GLUT2 in the BBM. Quantification of these changes using Western blotting of biotinylated surface-exposed protein showed a doubling of the expression of GLUT2 in the BBM, but the effects of glucose and GLP-2 were not additive. These results indicate that vascular GLP-2 can promote the insertion of GLUT2 into the rat jejunal BBM providing a low-affinity/high-capacity route of entry for dietary hexoses.

Enhancement of brush border membrane peptidase activity in rat jejunum induced by starvation

2000 ◽  
Vol 440 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Takashi Ihara ◽  
Tomoyuki Tsujikawa ◽  
Yoshihide Fujiyama ◽  
Hisao Ueyama ◽  
Iwao Ohkubo ◽  
...  
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Peptidase Activity ◽  
Rat Jejunum
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