Cardiac sarcolemmal purity is essential for the verification of adenylate cyclase inhibition via A1-adenosine receptors

1986 ◽  
Vol 333 (2) ◽  
pp. 156-162 ◽  
Author(s):  
Wolfgang Sch�tz ◽  
Michael Freissmuth ◽  
Viktoria Hausleithner ◽  
Elisabeth Tuisl
Keyword(s):  
Adenylate Cyclase ◽  
Adenosine Receptors ◽  
Adenylate Cyclase Inhibition ◽  
A1 Adenosine Receptors

Inhibition of brain adenylate cyclase by A1 adenosine receptors: Pharmacological characteristics and locations

1983 ◽  
Vol 267 (1) ◽  
pp. 123-129 ◽  
Author(s):  
C. Ebersolt ◽  
J. Premonta ◽  
A. Prochiantz ◽  
M. Perez ◽  
J. Bockaert
Keyword(s):  
Adenylate Cyclase ◽  
Adenosine Receptors ◽  
A1 Adenosine Receptors

Effect of temperature on atrial and ventricular adenosine A1 receptors of the guinea pig

1986 ◽  
Vol 64 (12) ◽  
pp. 1497-1502 ◽  
Author(s):  
Edward Leung ◽  
Elizabeth A. Woodcock
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Adenosine Receptors ◽  
Temperature Effects ◽  
Effect Of Temperature ◽  
Maximal Inhibition ◽  
Guinea Pig Heart ◽  
Adenylate Cyclase Inhibition ◽  
Adenosine A1 ◽  
Two Temperatures

Cardiac adenosine receptors are coupled to adenylate cyclase inhibition. In the guinea pig heart, the relative agonist potencies observed for adenylate cyclase inhibition were R-N6-phenylisopropyladenosine (R-PIA) = N6-cyclohexyladenosine > 5′-N-ethylcarboxamidoadenosine [Formula: see text]S-PIA. In both atrial and ventricular membranes, the antagonists 8-phenyltheophylline (8-PT) and isobutylmethylxanthine (IBMX) also showed similar affinities for atrial and ventricular adenosine receptors. The same pattern of relative agonist potencies was observed in experiments performed at either 25 or 37 °C. However, the maximal inhibition produced by R-PIA in atrial membranes decreased from 30.8 ± 3.2% (n = 7) at 25 °C to 18.8 ± 1.6% (n = 4) at 37 °C. No such difference in maximal inhibition was observed with ventricular membranes at these two temperatures (34.5 ± 1.6%, n = 6 at 25 °C and 35.3 ± 0.9%, n = 11 at37 °C). While there was no change in agonist potencies, the affinities of the antagonists 8-PT and IBMX at cardiac adenosine A1 receptors were affected by temperature. At 25 °C, the pKD values for 8-PT and IBMX in ventricular membranes were 4.65 ± 0.21 (n = 3) and 4.55 ± 0.20 (n = 3), respectively. Their affinities were 7-to 19-fold higher at 37 °C, the pKD values being 5.93 ± 0.12 (n = 7) (p < 0.02) and 5.38 ± 0.18 (n = 3) (p < 0.05), respectively. Over the same temperature range, the affinity of the muscarinic antagonist atropine was increased only by two-fold. These results suggest that (i) atrial and ventricular adenosine receptors are similar, (ii) atrial and ventricular adenylate cyclases are different in terms of temperature effects, and (iii) antagonist affinities at cardiac A1 receptors are critically dependent on temperature.

scholarly journals Species differences in structure-activity relationships of adenosine agonists and xanthine antagonists at brain A1 adenosine receptors

FEBS Letters ◽  
1986 ◽  
Vol 209 (1) ◽  
pp. 122-128 ◽  
Author(s):  
Dieter Ukena ◽  
Kenneth A. Jacobson ◽  
William L. Padgett ◽  
Cristina Ayala ◽  
Mah T. Shamim ◽  
...  
Keyword(s):  
Adenosine Receptors ◽  
Species Differences ◽  
Structure Activity Relationships ◽  
Structure Activity ◽  
Adenosine Agonists ◽  
A1 Adenosine Receptors

scholarly journals Immunolocalization of A1 Adenosine Receptors in Mammalian Spermatozoa

2000 ◽  
Vol 48 (9) ◽  
pp. 1163-1171 ◽  
Author(s):  
A. Minelli ◽  
C. Allegrucci ◽  
P. Piomboni ◽  
R. Mannucci ◽  
C. Lluis ◽  
...  
Keyword(s):  
Adenosine Receptors ◽  
A1 Adenosine Receptors ◽  
Mammalian Spermatozoa

Loss of A1 adenosine receptors in human temporal lobe epilepsy

1996 ◽  
Vol 710 (1-2) ◽  
pp. 56-68 ◽  
Author(s):  
M. Glass ◽  
R.L.M. Faull ◽  
J.Y. Bullock ◽  
K. Jansen ◽  
E.W. Mee ◽  
...  
Keyword(s):  
Temporal Lobe Epilepsy ◽  
Temporal Lobe ◽  
Adenosine Receptors ◽  
A1 Adenosine Receptors ◽  
Human Temporal Lobe
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