On-line estimation of cell growth for glutamic acid fermentation system

1983 ◽  
Vol 17 (3) ◽  
pp. 168-172 ◽  
Author(s):  
Sun Ho Park ◽  
Ki Tae Hong ◽  
Jae Heung Lee ◽  
Jong Chan Bae
Keyword(s):  
Cell Growth ◽  
Glutamic Acid ◽  
Acid Fermentation ◽  
Fermentation System ◽  
On Line

Optimization of the status of cell growth guided by an on-line biomass sensor for polymalic acid fermentation

2019 ◽  
Vol 79 ◽  
pp. 11-17
Author(s):  
Xiang Zou ◽  
Tianfu Li ◽  
Xiao Zhang ◽  
Hongqing Li ◽  
Bochu Wang
Keyword(s):  
Cell Growth ◽  
Acid Fermentation ◽  
Polymalic Acid ◽  
The Status ◽  
On Line

Thermal adaptation of acetic acid bacteria for practical high-temperature vinegar fermentation

2021 ◽  
Vol 85 (5) ◽  
pp. 1243-1251
Author(s):  
Nami Matsumoto ◽  
Naoki Osumi ◽  
Minenosuke Matsutani ◽  
Theerisara Phathanathavorn ◽  
Naoya Kataoka ◽  
...  
Keyword(s):  
Acetic Acid ◽  
High Temperature ◽  
Experimental Evolution ◽  
Culture Medium ◽  
Thermal Adaptation ◽  
Acetic Acid Bacteria ◽  
Acid Fermentation ◽  
Acetic Acid Production ◽  
Fermentation System ◽  
Fermentor Culture

ABSTRACT Thermotolerant microorganisms are useful for high-temperature fermentation. Several thermally adapted strains were previously obtained from Acetobacter pasteurianus in a nutrient-rich culture medium, while these adapted strains could not grow well at high temperature in the nutrient-poor practical culture medium, “rice moromi.” In this study, A. pasteurianus K-1034 originally capable of performing acetic acid fermentation in rice moromi was thermally adapted by experimental evolution using a “pseudo” rice moromi culture. The adapted strains thus obtained were confirmed to grow well in such the nutrient-poor media in flask or jar-fermentor culture up to 40 or 39 °C; the mutation sites of the strains were also determined. The high-temperature fermentation ability was also shown to be comparable with a low-nutrient adapted strain previously obtained. Using the practical fermentation system, “Acetofermenter,” acetic acid production was compared in the moromi culture; the results showed that the adapted strains efficiently perform practical vinegar production under high-temperature conditions.

scholarly journals Biochemical Effects of Fatty Acid and its Derivatives on L-Glutamic Acid Fermentation

1964 ◽  
Vol 28 (2) ◽  
pp. 114-119 ◽  
Author(s):  
Koichi TAKINAMI ◽  
Hiroshi OKADA ◽  
Toshinao TSUNODA
Keyword(s):  
Fatty Acid ◽  
Glutamic Acid ◽  
Acid Fermentation ◽  
Biochemical Effects

The uptake of amino acids by mouse cells (strain LS) during growth in batch culture and chemostat culture: the influence of cell growth rate

1967 ◽  
Vol 168 (1013) ◽  
pp. 421-438 ◽  
Keyword(s):  
Amino Acids ◽  
Growth Rate ◽  
Amino Acid ◽  
Cell Growth ◽  
Glutamic Acid ◽  
Batch Culture ◽  
Amino Acid Uptake ◽  
Essential Amino Acids ◽  
Growth Yields ◽  
Acid Uptake

The uptake of thirteen essential amino acids by mouse LS cells in suspension culture was determined by bacteriological assay methods. Chemostat continuous-flow cultures were used to determine the effect of different cell growth rates on the quantitative amino acid requirements for growth. The growth yields of the cells ( Y = g cell dry weight produced/g amino acid utilized) were calculated for each of the essential amino acids. A mixture of the non-essential amino acids, serine, alanine and glycine increased the cell yield from the essential amino acids. The growth yields from nearly all the essential amino acids in batch culture were increased when glutamic acid was substituted for the glutamine in the medium. The growth yields from the amino acids in batch culture were much less at the beginning than at the end of the culture. The highest efficiencies of conversion of amino acids to cell material were obtained by chemostat culture. When glutamic acid largely replaced the glutamine in the medium the conversion of amino acid nitrogen to cell nitrogen was 100 % efficient (that is, the theoretical yield was obtained) at the optimum growth rate (cell doubling time, 43 h). The maximum population density a given amino acid mixture will support can be calculated from the data. It is concluded that in several routinely used tissue culture media the cell growth is limited by the amino acid supply. In batch culture glutamine was wasted by (1) its spontaneous decomposition to pyrrolidone carboxylic acid and ammonia, and (2) its enzymic breakdown to glutamic acid and ammonia, but also glutamine was used less efficiently than glutamic acid. Study of the influence of cell growth rate on amino acid uptake rates per unit mass of cells indicated that a marked change in amino acid metabolism occurred at a specific growth rate of 0.4 day -1 (cell doubling time, 43 h). With decrease in specific growth rate below 0.4 day -1 there was a marked stimulation of amino acid uptake rate per cell and essential amino acids were consumed increasingly for functions other than synthesis of cell material.

Study of fault diagnoses expert system of glutamic acid fermentation process

2012 ◽  
Author(s):  
Guicheng Wang ◽  
Wang Xue ◽  
Ren Zhixin ◽  
Min Zhang ◽  
Zhang Zhansheng ◽  
...  
Keyword(s):  
Expert System ◽  
Glutamic Acid ◽  
Fermentation Process ◽  
Acid Fermentation

scholarly journals Promoting Cell Growth And Poly-Y-Glutamic Acid Production By Boosting The Synthesis of Alanine And D-Alanyl-D-Alanine In Bacillus Licheniformis

2022 ◽  
Author(s):  
Zheng Zhang ◽  
Penghui He ◽  
Shiying Hu ◽  
Yanqing Yu ◽  
Xiaoting Wang ◽  
...  
Keyword(s):  
Cell Growth ◽  
Microbial Biomass ◽  
Glutamic Acid ◽  
Bacillus Licheniformis ◽  
Alanine Racemase ◽  
High Yield ◽  
Effective Strategy ◽  
Genes Encoding ◽  
Alanine Synthesis ◽  
Glutamic Acid Production

Abstract Objective: The production of some bio-chemicals affected by the cell growth. This study aimed at promoting the cell growth by overexpressing the synthesis of peptidoglycans tetrapeptide tail components to improve poly-γ-glutamic acid (γ-PGA) production. Results: L-alanine, D-alanine and D-alanyl-D-alanine are primary precursors for the synthesis of peptidoglycans. The addition of L-alanine and D-alanine significantly increased both the cell growth and production of γ-PGA. Then, several genes encoding key enzymes for L/D-alanine and D-alanyl-D-alanine biosynthesis were overexpressed respectively, including ald (encoding alanine dehydrogenase), dal (encoding alanine racemase) and ddl (encoding D-alanine ligase). The results showed that the overexpression of genes ald , dal and ddl increased the production of γ-PGA by 19.72%, 15.91% and 60.90%, and increased the microbial biomass by 15.58%, 18.34% and 49.85%, respectively. Moreover, we demonstrated that the overexpression of genes ald , dal and ddl increased γ-PGA production mainly by enhancing cell growth rather than providing more precursors. Conclusions: This work illustrated the importance of the L/D-alanine and D-alanyl-D-alanine synthesis to the cell growth and the high yield of γ-PGA, and provided an effective strategy for producing γ-PGA .

Importance of Phosphoenolpyruvate Carboxylase of Corynebacterium glutamicum during the Temperature Triggered Glutamic Acid Fermentation

1999 ◽  
Vol 1 (4) ◽  
pp. 334-343 ◽  
Author(s):  
S. Delaunay ◽  
D. Uy ◽  
M.F. Baucher ◽  
J.M. Engasser ◽  
A. Guyonvarch ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Corynebacterium Glutamicum ◽  
Phosphoenolpyruvate Carboxylase ◽  
Acid Fermentation

scholarly journals L-GLUTAMIC ACID AND SUCCINIC ACID FERMENTATION BY BREVIBACTERIUM FLAVUM NO. 1996

1961 ◽  
Vol 7 (3) ◽  
pp. 177-191 ◽  
Author(s):  
HIROSHI OKADA ◽  
IWAO KAMEYAMA ◽  
SHINJI OKUMURA ◽  
TOSHINAO TSUNODA
Keyword(s):  
Glutamic Acid ◽  
Succinic Acid ◽  
Acid Fermentation ◽  
Brevibacterium Flavum ◽  
Succinic Acid Fermentation
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